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The Eca109 is a laboratory instrument used for cell culture analysis. It is designed to provide accurate and consistent measurements of cell growth, viability, and other parameters in a variety of cell types. The core function of the Eca109 is to facilitate the study of cell biology and biochemistry through the use of advanced imaging and data analysis technologies.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions) Kyse150, by Shanghai Institute of Biochemistry and Cell Biology (3 mentions) Kyse 180, by Shanghai Institute of Biochemistry and Cell Biology (2 mentions) Kyse 520, by Shanghai Institute of Biochemistry and Cell Biology (2 mentions) Kyse30, by Shanghai Institute of Biochemistry and Cell Biology (2 mentions) Eca9706, by Shanghai Institute of Biochemistry and Cell Biology (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Kyse 450, by Shanghai Institute of Biochemistry and Cell Biology (2 mentions) Eagle s minimum essential medium, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Growth supplements, by Cambrex (1 mentions) Het 1a, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions) Mycoalert mycoplasma detection kit, by Lonza (1 mentions) Kyse 450, by Thermo Fisher Scientific (1 mentions) Kyse30, by Thermo Fisher Scientific (1 mentions) Eca 109, by Thermo Fisher Scientific (1 mentions) Horseradish peroxidase hrp conjugated anti rabbit igg, by Jackson ImmunoResearch (1 mentions)

15 protocols using eca109

1

Cell Culture Conditions for KYSE150, TE-1, and ECA109

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The KYSE150, TE-1, and ECA109 were obtained from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). All cell lines were cultured in Dulbecco's modi ed Eagle's medium mixture with 10% fetal bovine serum. Cells were incubated at 37°C in a humidi ed atmosphere with 5% CO 2 .
Liu S., Lin Z., Wang J., Zheng Z., Rao W., Liu Z., Chen Y., Zeng Q., Li S., & Hu Z. (2021). Serum exosomal miR-4787-3p as potential lymph node metastasis and prognostic marker in esophageal squamous cell carcinoma.
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2

Culturing Human ESCC Cell Lines

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The human ESCC cell lines (TE-1D, KYSE-180, KYSE-520, ECA-109) were purchased from Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). The use of 293T cells gifted from Dr. Kang was approved by the research ethics committee of The Third Hospital, Nanchang university. All the cells were cultured in DMEM medium containing 10% fetal bovine serum (FBS) in the incubator with 5% CO2 at 37°C.
Zhou R., Tang X., Li L., Zhang F., Sun J., Ju C., Zhou Y., Liu R., Liang Y., Lv B., Zhang Z., Hu H, & Lv X.B. (2020). Identification of BRMS1L as Metastasis Suppressing Gene in Esophageal Squamous Cell Carcinoma. Cancer Management and Research, 12, 531-539.
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3

Esophageal Cancer Cell Line Culture

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Human ESCC cell lines Eca109, Kyse180, Kyse140, Kyse410, Kyse510, Kyse520, TE-1,
and Kyse30 were obtained from Shanghai Institute of Biochemistry and Cell
Biology (Shanghai, China) and were cultured in Eagle’s Minimum Essential Medium
(Gibco) supplemented with 10% fetal bovine serum (FBS; Sigma). A normal human
esophageal epithelial cell (Het-1A) was purchased from Cell Bank of Shanghai
Institutes for Biological Sciences (Chinese Academy of Sciences) and then
cultured in RPMI-1640 medium with 10% FBS. All cells were cultured at a
humidified incubator at 37 °C in an atmosphere of 5% CO2 and 95% air.
MiR-527 mimic, miR-527 inhibitor (miR-527-in), and negative control miRNAs were
purchased from GeneCopoeia Co Ltd.
Xian Q., Zhao R, & Fu J. (2020). MicroRNA-527 Induces Proliferation and Cell Cycle in Esophageal Squamous Cell Carcinoma Cells by Repressing PH Domain Leucine-Rich-Repeats Protein Phosphatase 2. Dose-Response, 18(2), 1559325820928687.
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4

Establishing Cell Lines for MMP12 Study

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Het-1A and ESCC cell lines (KYSE30, EC-1, Eca109 and EC9706) were bought from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). The cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) (Gibco BRL, USA) and maintained in a humidified incubator at 37°C with 5% CO2.
MMP12 small interfering RNA (si-MMP12) and the corresponding control RNA (si-NC) were purchased from Jinlai Biology (Beijing, China). Lipofectamine 3000 was applied for cellular transfection.
Mao J.T., Lu Q., Jing P.Y., Li Z.L., Yang X.Q., Zhang J.P, & Li Z. (2022). Comprehensive Analysis of Prognostic Value and Immune Infiltration of MMP12 in Esophageal Squamous Cell Carcinoma. Journal of Oncology, 2022, 4097428.
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5

Culturing Human Esophageal Cancer Cell Lines

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Human ESCC cell lines Eca109 and Eca9706 were purchased from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). Cells were maintained in Roswell Park Memorial Institute (RPMI) 1640 (HyClone) medium supplemented with 10% fetal bovine serum (GIBCO), 100 U/mL penicillin, and 100 μg/mL streptomycin, in a humidified atmosphere containing 5% CO2 at 37 °C.
Pang L., Li Q., Li S., He J., Cao W., Lan J., Sun B., Zou H., Wang C., Liu R., Wei C., Wei Y., Qi Y., Hu J., Liang W., Zhang W.J., Wan M, & Li F. (2016). Membrane type 1-matrix metalloproteinase induces epithelial-to-mesenchymal transition in esophageal squamous cell carcinoma: Observations from clinical and in vitro analyses. Scientific Reports, 6, 22179.
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6

ESCC Cell Line Cultivation

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The human ESCC cell lines Eca109 and TE13 were obtained from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). Cells were grown in RPMI 1640 (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (Gibco, Gaithersburg, MD, USA), 100 units of penicillin/ml, and 100 μg of streptomycin/ml (Invitrogen), and they were incubated at 37 °C in a humidified chamber supplemented with 5% CO2.
Ren L.H., Chen W.X., Li S., He X.Y., Zhang Z.M., Li M., Cao R.S., Hao B., Zhang H.J., Qiu H.Q, & Shi R.H. (2014). MicroRNA-183 promotes proliferation and invasion in oesophageal squamous cell carcinoma by targeting programmed cell death 4. British Journal of Cancer, 111(10), 2003-2013.
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7

Culturing Human ESCC Cell Lines

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Human ESCC cell lines Eca109 and TE-1 were purchased from Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). One human immortalized normal esophageal epithelial cell line (Het-1A), which was used as a ‘normal’ control for ESCC cell lines, was maintained in our laboratory. Eca109 and TE-1 cells were cultured in RPMI1640 (Hyclone) supplemented with 10% fetal bovine serum (Hyclone), 100 U/ml penicillin and 100 μg/ml streptomycin, within a humidified atmosphere containing 5% CO2 at 37°C. Het-1A cells were cultured in bronchial epithelial basal medium with growth supplements (Clonetics).
Zhao Z., Liu H., Yang Y., Sun K., Li M., Zhang J., Cai H, & Wang J. (2014). Expression of natriuretic peptide receptor-A in esophageal squamous cell carcinomas and the relationship with tumor invasion and migration. World Journal of Surgical Oncology, 12, 154.
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8

ESCC Cell Line Culture Protocol

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Human ESCC cell line TE13 and Eca109 were obtained from Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China) and cultured in Rosewell Park Memorial Institute (RPMI)-1640 medium with 10 % fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA) in a humidified incubator with 5.0 % CO2 at 37 °C. To avoid possible effects on gene expression, antibiotic was not used in cell culture.
He X., Zhang Z., Li M., Li S., Ren L., Zhu H., Xiao B, & Shi R. (2015). Expression and role of oncogenic miRNA-224 in esophageal squamous cell carcinoma. BMC Cancer, 15, 575.
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9

Cultivation of Human Esophageal Cancer Cell Lines

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Human esophageal carcinoma cell line Eca109 (derived from a Chinese patient with well-differentiated ESCC), KYSE150 (derived from a Japanese patient with poorly differentiated ESCC), and Eca9706 (derived from a Chinese patient with poorly differentiated ESCC) were purchased from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). Cells with 5 passages were maintained in Dulbecco's modified Eagle's medium (DMEM, HyClone Systems, Utah, USA) supplemented with 10% fetal bovine serum (FBS, GIBCO, California, USA), 100 units/mL penicillin and 100 mg/mL streptomycin. Cells were routinely incubated at 37°C under a 5% CO2 atmosphere.
Pang L., Li Q., Wei C., Zou H., Li S., Cao W., He J., Zhou Y., Ju X., Lan J., Wei Y., Wang C., Zhao W., Hu J., Jia W., Qi Y., Liu F., Jiang J., Li L., Zhao J., Liang W., Xie J, & Li F. (2014). TGF-β1/Smad Signaling Pathway Regulates Epithelial-to-Mesenchymal Transition in Esophageal Squamous Cell Carcinoma: In Vitro and Clinical Analyses of Cell Lines and Nomadic Kazakh Patients from Northwest Xinjiang, China. PLoS ONE, 9(12), e112300.
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10

Cell Line Characterization and Cultivation

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All cell lines (HEEC, Eca-109, TE-1, TE-10, KYSE-450, KYSE-410, and 293T) were purchased from Shanghai Institute of Cell Biology, Chinese Academy of Sciences, and identified by short tandem repeat sequence analysis. All cells were cultured in a humidified environment at 37 °C with 5% CO2. TP53 status of these cell lines is presented in Table S2.
Wang H., Song X., Wang Y., Yin X., Liang Y., Zhang T., Xu L., Jiang F, & Dong G. (2020). CircCNTNAP3-TP53-positive feedback loop suppresses malignant progression of esophageal squamous cell carcinoma. Cell Death & Disease, 11(11), 1010.
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