Zorbax stablebond sb c18

Quantification of anthocyanins was performed as previously described, with slight modifications [32 (link)]. The column used was a Zorbax Stablebond SB-C18 (4.6 × 250 mm, 5 μm) (Agilent Technologies, Santa Clara, CA, USA) and the gradient was as follows: 0–2 min: 5% B, 2–10 min: 5–20% B, 10–15 min: 20% B, 15–30 min: 20–25% B, 30–35 min: 25% B, 35–50 min: 25–33% B, 50–55 min: 33% B, 55–65 min: 33–36% B, 65–70 min: 36–45% B, 70–75 min: 45–53% B, 75–80 min: 53–55% B, 80–84 min: 55–70% B, 84–85 min: 70–5% B and 85–90 min: 5% B. Cyanidin 3-glucoside was used for quantification. Each sample was injected in triplicate.

Sixteen fatty acids standards—octanoic acid, capric acid, undecanoic acid, lauric acid, myristic acid, α-linolenic acid, linolic acid, pentadecanoic acid, palmitic acid, oleic acid, heptadecanoic acid, stearic acid, n-nonadecylic acid, arachidic acid, n-heneicosanoic acid, and behenic acid—were purchased from Sigma Reagent Co. (St. Louis, MO, USA). 2-(4-amino)-phenyl-1-hydrogen-phenanthrene [9,10-d] imidazole was synthesized in our laboratory, as described in our previous research paper [19 (link)]. All other analytical grade reagents were provided by Alltech Scientific (Beijing, China), unless otherwise stated.
A high-performance liquid chromatography (HPLC) system and Zorbax Stablebond XDB-C18, Zorbax StableBond SB-C18, Zorbax Stablebond Extend-C18, Zorbax C18, and Eclipse XDB-C18 were obtained from Agilent Technologies Co., Ltd., Palo Alto, CA, USA. Hypersil™ GOLD, Hypersil™ BDS C8, and Hypersil C18 were provided by Thermo Fisher Scientific, Waltham, MA, USA. We used the Agilent1260 series HPLC system equipped with an online degasser, a quaternary pump, an autosampler, a thermostat column compartment, and a fluorescence detection detector.