Human pRCC SK-RC-39 cell line and normal renal cell line HK2 were purchased from Cellcook Biotech (Guangzhou, China). The cell mediums (including RPM1-DMEM and RPM1-1640) and Fetal Bovine Serum (FBS) were purchased from Fcmacs Biotech (Nanjing, China). Antibiotic mixture (penicillin and streptomycin) and L-glutamine were purchased from Sangon Biotech (Shanghai, China). 57 mL FBS and 5.7 mL Antibiotic mixture as well as 5.7 mL L-glutamine were added into cell mediums to generate complete mediums. SK-RC-39 and HK2 were fed in complete 1640 and DMEM medium, respectively. Both the two kinds of cell lines were cultured in the 5% CO 2 incubator at 37℃.
Fetal bovine serum (fbs)
Manufactured by Fcmacs
Sourced in China
Fetal Bovine Serum (FBS) is a widely used cell culture supplement derived from the blood of bovine fetuses. It is a complex mixture of proteins, growth factors, and other components that supports the growth and proliferation of various cell types in vitro. FBS provides essential nutrients and stimulants for cell culture applications.
Automatically generated - may contain errors
2 protocols using fetal bovine serum (fbs)
1
Cultivation of pRCC and Normal Renal Cell Lines
2
Culturing SK-RC-39 and HK-2 Cell Lines
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Human pRCC SK-RC-39 cell line and normal renal cell line HK-2 were purchased from Cellcook Biotech (Guangzhou, China). The cell mediums (including RPM1-DMEM and RPM1-1640) and Fetal Bovine Serum (FBS) were purchased from Fcmacs Biotech (Nanjing, China). Antibiotic mixture (penicillin and streptomycin) and l -glutamine were purchased from Sangon Biotech (Shanghai, China). 57 mL FBS and 5.7 mL Antibiotic mixture as well as 5.7 mL l -glutamine were added into cell mediums to generate complete mediums. SK-RC-39 and HK-2 were fed in complete 1640 and DMEM medium, respectively. Both the two kinds of cell lines were cultured in the 5% CO2 incubator at 37 °C.
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