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Non specific shrna

Manufactured by Santa Cruz Biotechnology
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Non-specific shRNA is a laboratory tool used in gene silencing experiments. It functions by introducing short hairpin RNA (shRNA) sequences into cells, which can then trigger the degradation or translational repression of targeted mRNA molecules. This product is designed to be non-specific, meaning it is not targeted towards any particular gene.

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Lab products found in correlation

Pbabe puro mcherry egfp lc3b, by Addgene (1 mentions) Copgfp, by Santa Cruz Biotechnology (1 mentions) Pbabepuro gfp lc3, by Addgene (1 mentions)

2 protocols using non specific shrna

1

Engineered Plasmids for Autophagy Analysis

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The plasmids pBABE-puro mCherry-EGFP-LC3B and pBABEpuro GFP-LC3 (N#22418 and 22405), engineered by Jayanta Debnath [21] , were obtained from Addgene. Cell transduction and selection were performed as previously described [20] (link), [22] (link). Genetic inhibition of autophagy was achieved using ATG7 shRNA-mediated knock down (Santa Cruz). Non-specific shRNA (control) as well as copGFP were applied according to manufacturer's instructions (Santa Cruz). Transduction was performed using lentiviral particles with up to five distinct expression constructs as described elsewhere [20] (link). Transduction efficiency was quantified by the number of GFP-positive cells and in general exceeded 94% at the end of puromycin selection.
Klose J., Stankov M.V., Kleine M., Ramackers W., Panayotova-Dimitrova D., Jäger M.D., Klempnauer J., Winkler M., Bektas H., Behrens G.M, & Vondran F.W. (2014). Inhibition of Autophagic Flux by Salinomycin Results in Anti-Cancer Effect in Hepatocellular Carcinoma Cells. PLoS ONE, 9(5), e95970.
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2

Neonatal Rat Ventricular Myocyte Knockdown

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Control (nontransfected) neonatal rat ventricular myocytes were isolated and plated on collagen-coated chamber slides for 24 hours before being infected with anti-SAP97 shRNA lentiviral particles (7 μg/ml, Santa Cruz Biotechnology) in a polybrene mixture (5 μg/ml) (12 hours, 37°C). At 72 hours after transduction, cultures were fixed in 4% paraformaldehyde and analyzed by immunofluorescence (as described in Supplementary Materials and Methods). Nonspecific shRNA (Santa Cruz Biotechnology) was used as a negative control.
Asimaki A., Kapoor S., Plovie E., Arndt A.K., Adams E., Liu Z., James C.A., Judge D.P., Calkins H., Churko J., Wu J.C., MacRae C.A., Kléber A.G, & Saffitz J.E. (2014). Identification of a New Modulator of the Intercalated Disc in a Zebrafish Model of Arrhythmogenic Cardiomyopathy. Science translational medicine, 6(240), 240ra74.
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