Four micron-thick paraffin sections were prepared. Following deparaffinization, rehydration, and antigen retrieval, a biotin block and a serum-free protein block were performed (Superblock or Mouse-to-Mouse Block; ScyTek Laboratories, Logan, UT). The slides were incubated overnight at 4°C with polyclonal rabbit RNase 6 antibody (Abcam) or polyclonal RNase 7 antibody (Sigma), washed, and incubated with antipolyvalent biotinylated antibody and UltraTek Streptavidin/HRP (ScyTek Laboratories). Sections were developed using 3-Amino-9-Ethylcarbazole and counterstained with hematoxylin. Negative-control sections were incubated with non-immune serum in place of RNase 6 or RNase 7 antibody.
Mouse to mouse block
Manufactured by ScyTek Laboratories
The Mouse-to-Mouse Block is a laboratory device designed to facilitate the transfer of samples between mice. It provides a secure and controlled environment for the transference of materials, ensuring the safety and integrity of the samples during the process.
Automatically generated - may contain errors
2 protocols using mouse to mouse block
1
Immunohistochemical Analysis of RNase 6 and RNase 7
2
Immunohistochemical Analysis of RNase 6 and RNase 7
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