Nf κb p105 p50

Manufactured by Cell Signaling Technology

Sourced in United States

NF-κB p105/p50 is a protein that plays a crucial role in the NF-κB signaling pathway. It functions as a transcription factor that regulates the expression of genes involved in immune response, inflammation, and cell survival.

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Lab products found in correlation

Las af software, by Leica (1 mentions) Af6000 fluorescent microscope, by Leica (1 mentions) Nf κb p65 c22b4, by Cell Signaling Technology (1 mentions) Sds page gel, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Immobilon western chemiluminescent hrp substrate, by Merck Group (1 mentions) Image lab system, by Bio-Rad (1 mentions) Nf κb p65, by Cell Signaling Technology (1 mentions) Phospho nf κb p65, by Cell Signaling Technology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

NF-κB p50 NF-κB

3 protocols using nf κb p105 p50

Lymphotoxin Stimulation of Fibroblasts

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Fibroblasts of patient (P2) and healthy donor were stimulated with 100 ng ml⁻¹ of lymphotoxin α1/β2 (R&D Systems, 678-LY-010) for 4 h. After stimulation, cells were fixed with 4% formaldehyde in PBS for 30 min and then blocked and permeabilized with solution containing 10% FCS plus 0.1% Triton X-100. Cells were immunostained with DAPI and rabbit antibodies against NFκB2 (p100/p52) (Cell Signaling, 3017) and NF-κB (p105/p50) (Cell Signaling, 3035) at a dilution of 1:100, respectively, and afterwards with anti-rabbit Alexa Fluor 546-conjugated antibody at a dilution of 1:500 (Life Technologies, A10040). Images were acquired on a Leica AF6000 fluorescent microscope using Leica LASAF software for acquisition. Images were taken at × 64 magnification.

Willmann K.L., Klaver S., Doğu F., Santos-Valente E., Garncarz W., Bilic I., Mace E., Salzer E., Domínguez Conde C., Sic H., Májek P., Banerjee P.P., Vladimer G.I., Haskoloğlu Ş., Gökalp Bolkent M., Küpesiz A., Condino-Neto A., Colinge J., Superti-Furga G., Pickl W.F., van Zelm M.C., Eibel H., Orange J.S., Ikincioğulları A, & Boztuğ K. (2014). Biallelic loss-of-function mutation in NIK causes a primary immunodeficiency with multifaceted aberrant lymphoid immunity. Nature Communications, 5, 5360.

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Western Blot Analysis of Protein Expression

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Whole cell lysate (30 μg/lane), nuclear (10 μg/lane), and cytoplasmic (30 μg/lane) fractions were loaded to a SDS-PAGE gel (Invitrogen, Carlsbad, CA, USA) and separated by electrophoresis. After transfer of the separated proteins onto a piece of PVDF membrane (Millipore, Billerica, MA, USA), the membrane was probed by Western blot. Briefly, the membrane was blocked with 5% nonfat milk for 1 h at room temperature, reacted with a primary antibody (1:1000–2000 dilution) overnight at 4 °C, and followed by incubating at room temperature for 1 h with an HRP-conjugated anti-rabbit IgG secondary antibody (Abmart, Berkeley Heights, NJ, USA). The following primary antibodies were used: IRF-1 (B-1), IRF-8 (C-19) (Santa Cruz Biotech, Santa Cruz, CA, USA), NF-κB p105/p50 (#3035), NF-κB p65 (C22B4), I-κBα (#9247), IRF-3 (D83B9), lamin B1 (D4Q4Z) (Cell Signaling, Beverly, MA, USA), IRF-5 (10T1) (Abcam, Cambridge, MA, USA). The membranes were developed using Immobilon™ Western Chemiluminescent HRP Substrate (Millipore) and the images were acquired using The G-Box fluorescence and Chemiluminescence of imaging system (Syngene, England).

Wang F., Qiao L., Lv X., Trivett A., Yang R., Oppenheim J.J., Yang D, & Zhang N. (2016). Alarmin human α defensin HNP1 activates plasmacytoid dendritic cells by triggering NF-κB and IRF1 signaling pathways. Cytokine, 83, 53-60.

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Analyzing Protein Expression in Renal Cortex

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The proteins from renal cortical tissues were extracted with RIPA lysis buffer. The samples were subjected to Western blot analysis with primary antibodies against phospho-NF-κB p65, NF-κB p65, IKKβ, NF-κB p105/p50 (Cell Signaling Technology, MA, United States), RXRα (Abcam, MA, United States), PXR, and GAPDH (Santa Cruz Biotechnology, CA, United States) overnight at 4°C and subsequently incubated with the conjugated secondary antibody of horseradish peroxidase-labeled anti-rabbit IgG or anti-mouse IgG (Cell Signaling Technology, MA, United States) at room temperature. Target protein expression was detected by using an Image Lab System (Bio-Rad Laboratories Inc., Hercules, CA, United States) and analyzed with ImageJ software.

Dou J.Y., Zhang M., Cen H., Chen Y.Q., Wu Y.F., Lu F., Zhou J., Liu X.S, & Gu Y.Y. (2022). Salvia miltiorrhiza Bunge (Danshen) and Bioactive Compound Tanshinone IIA Alleviates Cisplatin-Induced Acute Kidney Injury Through Regulating PXR/NF-κB Signaling. Frontiers in Pharmacology, 13, 860383.

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