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Fluoview 1000 ix2 upright confocal microscope

Manufactured by Olympus
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The FluoView 1000 IX2 is an upright confocal microscope designed for high-resolution, multi-dimensional imaging. It utilizes scanning laser technology to capture detailed fluorescence images of biological samples.

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Lab products found in correlation

Cftm594 tunel apoptosis detection kit, by Biotium (2 mentions) C57bl 6 mice, by Charles River Laboratories (2 mentions) Anti rip140 sc 8997, by Santa Cruz Biotechnology (2 mentions) Anti ip3r sc 271197, by Santa Cruz Biotechnology (2 mentions) Ls c121863, by LifeSpan BioSciences (2 mentions) Duolink pla assay kit, by Olink (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Ab144p, by Merck Group (1 mentions)

Close spelling variants of this product

Fluoview Upright Confocal Microscope FluoView 1000 IX2 Fluoview 1000 confocal microscope Fluoview 1000 microscope FluoView confocal microscope IX2 microscope Fluoview 1000 Upright microscope Confocal microscope

6 protocols using fluoview 1000 ix2 upright confocal microscope

1

Investigating RIP140 Role in ER Stress-Induced Apoptosis

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Male adult C57BL/6 mice (8-9 weeks old), from Charles River Laboratories, were maintained and experimental procedures were conducted according to NIH guidelines and approved by the University of Minnesota Institutional Animal Care and Use Committee (Protocol no. 1007A86332). Lentivirus carrying RIP140-shRNA, was delivered to hippocampus using stereotaxic apparatus at anteroposterior (AP) 2.0 mm, medial-lateral (ML) 1.2 mm and dorsoventral (DV)1.6 mm according to previous describe65 . RIP140 protein levels following virus injection were detected using immunofluorescence and western blot as indicated in the figure legend. The ER stress animal models were generated following intraperitoneal injection of Tg(1 μg/g) or normal saline(control) after 3 or 18 days of virus injection. Then the animals were anesthetized 2 days later and brain tissues were subjected to immunofluorescence staining or protein extraction. To detect the apoptotic cells, TUNEL staining of brain sections was performed using CFTM594 TUNEL apoptosis detection kit (30064, Biotium). Images were acquired by Olympus FluoView 1000 IX2 upright confocal microscope. The TUNEL-positive cell numnber versus total cell number indicated by DAPI from the Lentivirus-infected areas was counted and quantified. Background was adjusted using Image J before counting.
Feng X., Krogh K.A., Wu C.Y., Lin Y.W., Tsai H.C., Thayer S.A, & Wei L.N. (2014). Receptor interacting protein 140 attenuates endoplasmic reticulum stress in neurons and protects against cell death. Nature communications, 5, 4487.
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2

Immunofluorescence Staining of Cells

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Cells were fixed by 4% paraformaldehyde for 10 minutes and permeabilized using 0.2% Triton X-100 for 5 minutes at 4°C. Nonspecific binding was blocked by incubation of cells with 0.5% bovine serum albumin (BSA)-PBS 1 hour before incubation with primary antibodies. IF was performed using as primary antibody rabbit anti-PAX6 IgG (Abcam, ab5790), goat anti-MAP2 (Santa Cruz Biotechnology, sc-5359), Rabbit anti-RIP140 (Santa Cruz Biotechnology, sc-8997), or mouse anti-LSD1 (Santa Cruz Biotechnology, sc-271720) and as secondary antibody FICT-conjugated donkey anti-Rabbit IgG, Cy3-conjugated donkey anti-Rabbit IgG (Santa Cruz Biotechnology), Cy3-conjugated donkey anti-mouse IgG (Santa Cruz Biotechnology), and Cy3-conjugated donkey anti-goat IgG (Santa Cruz Biotechnology). Nuclei were stained by DAPI (Sigma-Aldrich). Images were acquired with an Olympus FluoView 1000 IX2 upright confocal microscope (OLYMPUS, New York, NY, http://www.olympusamerica.com/).
Wu C.Y., Persaud S.D, & Wei L.N. (2015). Retinoic Acid Induces Ubiquitination-Resistant RIP140/LSD1 Complex to Fine-Tune Pax6 Gene in Neuronal Differentiation. Stem cells (Dayton, Ohio), 34(1), 114-123.
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3

Endogenous Protein Complex Detection

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In situ proximal-ligation assay was performed using Duolink PLA assay kit (Olink Bioscience) according to the protocol provided by the manufacturer. Briefly, cells were fixed using 4% poly-paraformaldehyde and incubated with primary antibodies overnight at 4°C. To detect the endogenous interaction of RIP140 with IP3R1, anti-RIP140 (sc-8997; Santa Cruz; 1:200) and anti-IP3R (sc-271197; Santa Cruz;1:200) were used. The IP3R1 C-and N-terminal association was detected using antibodies recognize C-(407140, Calbiochem; 1:300) and N-terminus(LS-C121863, Lifespan; 1:300) of IP3R1. Images were acquired by Olympus FluoView 1000 IX2 upright confocal microscope. The fluorescent punctae represents protein complex from different fields of individual experiment was counted with Image J image processing freeware (http://rsbweb.nih.gov/ij/), and the result was analyzed using SPSS17.0 software.
Feng X., Krogh K.A., Wu C.Y., Lin Y.W., Tsai H.C., Thayer S.A, & Wei L.N. (2014). Receptor interacting protein 140 attenuates endoplasmic reticulum stress in neurons and protects against cell death. Nature communications, 5, 4487.
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4

Immunolabeling of Spinal Cord Tissues in Mice

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Eight week old mice were perfused with PBS containing 4% paraformaldehyde. Lumbar spines were removed, fixed in 4% paraformaldehyde for 2 h, and immersed in 30% sucrose for 24 h at 4 °C. Coronal sections were obtained in 20-μm-thick slices. PBS-washed slices were treated with a blocking solution containing 0.2% Triton X-100, 1% bovine serum albumin, and 5% goat serum in PBS for 60 min at room temperature, incubated with primary antibodies, including CRABP1 (C1608, Sigma–Aldrich; 1:400), ChAT (AB144P, Millipore-Sigma; 1:1000), and GFAP (AB5804; Millipore–Sigma, 1:1000) diluted in blocking solution at 4 °C overnight, and incubated with fluorochrome-conjugated secondary antibody and 4′,6-diamidino-2-phenylindole in the dark for 1 h. Fluorescent images were acquired under an Olympus FluoView 1000 IX2 upright confocal microscope.
Lin Y.L., Lin Y.W., Nhieu J., Zhang X, & Wei L.N. (2020). Sonic Hedgehog-Gli1 Signaling and Cellular Retinoic Acid Binding Protein 1 Gene Regulation in Motor Neuron Differentiation and Diseases. International Journal of Molecular Sciences, 21(11), 4125.
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5

Investigating RIP140 Role in ER Stress-Induced Apoptosis

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Male adult C57BL/6 mice (8-9 weeks old), from Charles River Laboratories, were maintained and experimental procedures were conducted according to NIH guidelines and approved by the University of Minnesota Institutional Animal Care and Use Committee (Protocol no. 1007A86332). Lentivirus carrying RIP140-shRNA, was delivered to hippocampus using stereotaxic apparatus at anteroposterior (AP) 2.0 mm, medial-lateral (ML) 1.2 mm and dorsoventral (DV)1.6 mm according to previous describe65 . RIP140 protein levels following virus injection were detected using immunofluorescence and western blot as indicated in the figure legend. The ER stress animal models were generated following intraperitoneal injection of Tg(1 μg/g) or normal saline(control) after 3 or 18 days of virus injection. Then the animals were anesthetized 2 days later and brain tissues were subjected to immunofluorescence staining or protein extraction. To detect the apoptotic cells, TUNEL staining of brain sections was performed using CFTM594 TUNEL apoptosis detection kit (30064, Biotium). Images were acquired by Olympus FluoView 1000 IX2 upright confocal microscope. The TUNEL-positive cell numnber versus total cell number indicated by DAPI from the Lentivirus-infected areas was counted and quantified. Background was adjusted using Image J before counting.
Feng X., Krogh K.A., Wu C.Y., Lin Y.W., Tsai H.C., Thayer S.A, & Wei L.N. (2014). Receptor interacting protein 140 attenuates endoplasmic reticulum stress in neurons and protects against cell death. Nature communications, 5, 4487.
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6

Endogenous Protein Complex Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
In situ proximal-ligation assay was performed using Duolink PLA assay kit (Olink Bioscience) according to the protocol provided by the manufacturer. Briefly, cells were fixed using 4% poly-paraformaldehyde and incubated with primary antibodies overnight at 4°C. To detect the endogenous interaction of RIP140 with IP3R1, anti-RIP140 (sc-8997; Santa Cruz; 1:200) and anti-IP3R (sc-271197; Santa Cruz;1:200) were used. The IP3R1 C-and N-terminal association was detected using antibodies recognize C-(407140, Calbiochem; 1:300) and N-terminus(LS-C121863, Lifespan; 1:300) of IP3R1. Images were acquired by Olympus FluoView 1000 IX2 upright confocal microscope. The fluorescent punctae represents protein complex from different fields of individual experiment was counted with Image J image processing freeware (http://rsbweb.nih.gov/ij/), and the result was analyzed using SPSS17.0 software.
Feng X., Krogh K.A., Wu C.Y., Lin Y.W., Tsai H.C., Thayer S.A, & Wei L.N. (2014). Receptor interacting protein 140 attenuates endoplasmic reticulum stress in neurons and protects against cell death. Nature communications, 5, 4487.
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