Mouse anti nkx2 1 antibody

Primary antibodies used were mouse anti-NKX2-1 antibody diluted at 1:100 (LS Bio; Seattle, WA, USA); rabbit anti-SOX2 antibody diluted at 1:500 (Novus Biologicals; Littleton, CO, USA); rabbit anti-K5 antibody diluted at 1:250 (Abcam; Cambridge, UK); rabbit anti-K8 antibody diluted at 1:250 (LS Bio; Seattle, WA, USA); rabbit anti-K18 antibody diluted at 1:250 (LS Bio; Seattle, WA, USA); rabbit anti-K14 antibody diluted at 1:250 (ProteinTech; Chicago, IL, USA); rabbit anti-FOXA2 antibody diluted at 1:2000 (Abcam; Cambridge, UK). Sections were incubated with the primary antibodies at 4 °C overnight. Secondary antibodies used were anti-mouse and anti-rabbit Ig ImmPress reagent kit peroxidase (Vector Laboratories; Peterborough, UK). Antigen detection was performed using a DAB kit (Vector Laboratories; Peterborough, UK). Slides were counterstained using hematoxylin.

Primary antibodies used were mouse anti-NKX2-1 antibody diluted at 1:100 (LS Bio; Seattle, WA, USA), rabbit anti-SOX2 antibody diluted at 1:500 (Novus Biologicals; Littleton, CO, USA), rabbit anti-K8 antibody (LS Bio; Seattle, WA, USA) diluted at 1:250; mouse anti-p63 antibody (Santa Cruz Biotechnology; Dallas, Texas, USA) diluted at 1:200. Sections were incubated at 4 °C overnight. Secondary antibodies used were Cy3-conjugated anti-mouse antibodies (Jackson ImmunoResearch; West Grove, PA, USA) diluted at 1:200; FITC-conjugated anti-rabbit antibodies (Jackson ImmunoR-esearch; West Grove, PA, USA), diluted at 1:100 and applied 1 h at room temperature (RT). Slides were mounted using Vectashield (Vector Laboratories; Peterborough, UK). In case of whole mount IF staining, dissected respiratory structures were treated with rabbit anti-K8 antibody (LS Bio; Seattle, WA, USA) diluted at 1:250 overnight at RT and by secondary antibody Cy3-conjugated anti-rabbit antibody (Jackson ImmunoResearch; West Grove, PA, USA), diluted at 1:200, applied 4 °C/overnight. Slides were mounted using Vectashield (Vector Laboratories; Peterborough, UK).