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Iba1 antibody 019 19741

Manufactured by Fujifilm

The Iba1 antibody (019-19741) is a laboratory reagent produced by Fujifilm. It is a polyclonal antibody that specifically binds to the Iba1 protein, which is commonly used as a marker for microglia in research applications.

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2 protocols using iba1 antibody 019 19741

1

Antibody Validation for Western Blot and IHC

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Antibodies were utilized for western blot (WB) and/or immunohistochemistry (IHC). The COX1 antibody (160110) was purchased from Cayman Chemicals. The COX2 (15191) and APP (32136) antibodies were purchased from Abcam. The Iba1 antibody (019-19741) was purchased from Wako Chemicals. The GFAP (D1F4Q), BACE (D10E5), PSD95 (2507S) and Arg1 (D4E3M) antibodies were purchased from Cell Signaling. The synaptophysin antibody (mab5258) was purchased from Millipore. The iNOS (N20) and GAPDH (6C5) antibodies were purchased from Santa Cruz Biotechnology.
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2

Immunofluorescence Antibody Protocol

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The mouse monoclonal RFP antibody (6G6) was purchased from ChromoTek, the chicken polyclonal GFP antibody (GFP-1020) from Aves Labs (Tigard), the rabbit polyclonal GFAP antibody (AB5804) and the mouse monoclonal NeuN antibody (MAB377) from Millipore, the rabbit polyclonal IBA1 antibody (019–19741) from Wako Chemicals, and the rabbit polyclonal mGluR5 antibody (AGC-007) was from Alomone Labs. The goat anti-mouse IgG2a-Cy3, goat anti-chicken-Cy2, goat anti-rabbit-Cy5, goat anti-mouse IgG1-Cy5 and donkey anti-rabbit IgG-Alexa Fluor 647 antibodies were purchased from Jackson ImmunoResearch Laboratories.
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