Columbia sheep blood agar plates

Ten μl of BHI broth was inoculated using micropipette on 5% Columbia sheep blood agar plates (HiMedia Laboratories Pvt Ltd, Mumbai, India). The inoculation loop was heated on the blue flame of Bunsen burner, till it became red hot and was allowed to cool down at room temperature. Using loop, streaking was done on agar plates and was placed in incubator at 37°C for 24 h. Then, the bacterial growth was counted as CFUs using manual counting technique.

The paper points were transferred immediately to the sterile test tubes. Within 10 minutes, the BHI broth was transferred to the microbiological lab. Ten microliters of the BHI broth were inoculated using micropipette on 5% Columbia sheep blood agar plates (HiMedia Laboratories Pvt. Ltd., Mumbai, India). The inoculation loop was heated on the blue flame of Bunsen burner, till it became red hot. The loop was allowed to cool at room temperature and streaking was done on the agar plates.[23 (link)] The agar plates were placed in the incubator at 37°C for 24 h. After 24 h, the bacterial growth was counted as CFUs using manual counting technique.