Two cancer cell lines derived from human breast cancer, estrogen receptor (ER)-positive and HER2-negative MCF7 cells and ER-negative and HER2-overexpressing SKBR3 cells were provided by the Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University. The other two cell lines derived from human breast cancer, ER-negative and HER2-negative MDA-MB-231 cells and ER-positive and HER2-overexpressing BT474 cells, were purchased from the American Type Culture Collection (Rockville, MD, USA). These breast cancer cells were seeded in 75-cm2 dishes (Becton-Dickinson, Franklin Lakes, NJ, USA) and cultured in 10 ml of medium at 37ºC in a humidified atmosphere of 5% CO2. These cells were grown in DMEM (Invitrogen, Carlsbad, CA, USA) supplemented with 10% heat-inactivated fetal bovine serum (Nichirei Bioscience Inc., Japan), 100 IU/ml penicillin, 100 mg/ml streptomycin (Invitrogen), 2 mM glutamine (Nissui Pharmaceutical Co., Ltd., Japan), and 0.5 mM sodium pyruvate. Cells were grown to confluence and harvested by trypsinization with 0.25 mg/ml trypsin/EDTA solution (Invitrogen) and suspended in culture medium before use.
75 cm2 dishes
Manufactured by BD
Sourced in United States, Japan
The 75-cm2 dishes are a type of lab equipment used for cell culture applications. They provide a surface area of 75 square centimeters for the growth and maintenance of cells in a controlled laboratory environment.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Heat inactivated fetal bovine serum, by Nichirei Biosciences (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Trypsin edta solution, by Thermo Fisher Scientific (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Bt474, by American Type Culture Collection (1 mentions)
Glutamine, by Nissui Pharmaceutical (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
2 protocols using 75 cm2 dishes
1
Breast Cancer Cell Line Cultivation
2
Establishment and Culture of Gastric Cancer Cell Lines
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OCUM-2MD3, a cell line derived from a human scirrhous gastric cancer, with high peritoneal-seeding activity, was kindly provided by the Department of Surgical Oncology of Osaka City University of Medicine. NUGC-3, a cell line derived from human poorly differentiated gastric cancer, was purchased from National Institute of Biomedical Innovation (Osaka, Japan). Cells were seeded in 75-cm2 dishes (Becton–Dickinson, Tokyo, Japan). OCUM-2MD3 cells were cultured in 10-mL Dulbecco’s modified Eagle’s medium (DMEM, Life Technologies, Tokyo, Japan) supplemented with 10 % heat-inactivated fetal bovine serum (FBS) (Nichirei Bioscience Inc., Tokyo, Japan), 100 IU/mL penicillin, 100 mg/mL streptomycin (Life Technologies Tokyo, Japan), at 37 °C in a humidified atmosphere of 5 % CO2 in air. The culture medium for NUGC-3 cells was Roswell Park Memorial Institute (RPMI) 1640 medium (Life Technologies) with 15 % FBS, and the other additives were same as above.
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