The optical rotations were obtained with a JASCO P-1010 digital polarimeter. The 1H and 13C-NMR spectra were recorded on a JEOL α 500 FT NMR spectrometer. The chemical shifts were expressed in δ (ppm) and the coupling constants (J) in Hz. The electrospray ionization mass spectrometry (ESIMS) data were obtained on a PE QSTAR mass spectrometer and the infrared (IR) spectra were recorded on a DR 8020 Shimadzu spectrophotometer. The HPLC was performed on a Hitachi L-6000 pump equipped with a Shodex RI-101 monitor and a Hitachi L-4000 UV detector, using a Cosmosil 5C18AR-II (5 µm) or a Mightysil RP-18 (5 µm) column. Merck silica gel 60 (0.063–0.20 mm) was used for column chromatography. The analytical thin layer chromatography (TLC) was performed on commercial silica gel 60 F254 visualized with vanillin–EtOH-1% H2SO4. All solvents used were reagent grade.
P 1010 digital
Manufactured by Jasco
Sourced in Japan
The P-1010 digital is a laboratory equipment product. It is a digital device designed for use in scientific research and analysis. The core function of the P-1010 digital is to provide precise measurements and data collection capabilities for laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Kieselgel 60, by Merck Group (2 mentions)
Caffeic acid, by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
3200 qtrap, by AB Sciex (1 mentions)
Vnmrs 500 spectrometer, by Agilent Technologies (1 mentions)
Ft ir 4100 spectrometer, by Jasco (1 mentions)
Synapt g2, by Waters Corporation (1 mentions)
Uv 530 ultraviolet spectrophotometers, by Jasco (1 mentions)
L 7110 pump, by Hitachi (1 mentions)
Apex 2 mass spectrometer, by Bruker (1 mentions)
L 2455 photodiode array detector, by Hitachi (1 mentions)
Ascend 400, by Bruker (1 mentions)
6120 quadrupole lc ms instrument, by Agilent Technologies (1 mentions)
Kieselgel 60 rp 18, by Merck Group (1 mentions)
Kieselgel 60 f254, by Merck Group (1 mentions)
6 protocols using p 1010 digital
1
Analytical Characterization of Organic Compounds
2
Analytical Characterization of Natural Compounds
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Optical rotations were measured in MeOH on a P-1010 digital polarimeter (Jasco, Tokyo, Japan), 1H and 13C NMR spectra were recorded at 500 and 400 and 125 and 1000 MHz in on Varian (Palo Alto, CA, USA) and Bruker (Karlsruhe, Germany). The same solvent was used as internal standard. The multiplicities were determined by DEPT spectrum [60 ] COSY, HSQC, HMBC and NOESY spectra were recorded using Bruker microprograms. ESI MS spectra were recorded on a 6120 Quadrupole LC/MS instruments (Agilent Technologies, Milan, Italy), respectively. Analytical and preparative TLC were performed on silica gel (Kieselgel 60, F254, 0.25 and 0.5 mm respectively) and on reversed phase (Kieselgel 60 RP-18, F254, 0.20 mm) plates (Merck, Darmstadt, Germany). The spots were visualized by exposure to UV radiation (253 nm), or by spraying first with 10% H2SO4 in MeOH and then with 5% phosphomolybdic acid in EtOH, followed by heating at 110 °C for 10 min. Column chromatography was performed using silica gel (Merck, Kieselgel 60, 0.063–0.200 mm). Quercetin and caffeic acid were purchased from Sigma-Aldrich Milano, Italy)
3
Spectroscopic Characterization of Compounds
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Optical rotations were measured on a JASCO P-1010 digital polarimeter (Tokyo, Japan) with a 5 cm cell. IR spectra were recorded on a JASCO FT/IR 4100 spectrometer. The 1D and 2D NMR spectra were obtained using a Varian VNMRS 500 spectrometer (Palo Alto, CA, USA) in a CD3OD solvent and CDCl3. High-resolution ESI mass spectra were acquired using a Waters SYNAPT G2, courtesy of Korea Basic Science Institute (Ochang Center, Korea). The ESI-MS/MS spectra were obtained in the enhanced product ion scan mode on an ABSCIEX QTRAP 3200 (Foster, CA, USA).
4
Spectroscopic Analysis of Chemical Compounds
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Optical rotation spectra were obtained on a Jasco P-1010 digital polarimeter (Jasco, Tokyo, Japan). UV spectra were recorded using Jasco UV-530 ultraviolet spectrophotometers. IR spectra were detected on the Varian 1000 Scimitar series FT-IR (Varian Inc., Palo Alto, CA, USA). NMR spectroscopic analysis was performed on a 400 or a 500 MHz Varian Mercury Plus FT-NMR (Varian Inc., Palo Alto, CA, USA). HRESIMS data were obtained on a Bruker Daltonics Apex II mass spectrometer (Bruker Daltonics, Bremen, Germany). Silica gel 60, 70–230/230–400 μm ASTM (Merck, Darmstadt, Germany) was used for the normal phase column chromatography. A Hitachi L-7110 pump coupled a Hitachi L-2455 Photodiode Array Detector (Hitachi, Tokyo, Japan) and a column Hibar RT250-10 mm, LiChrosper Si 60, 5 μm (Merck, Darmstadt, Germany) were used for normal phase HPLC chromatography. All methods were conducted following the relevant regulations and guidelines.
5
Optical Rotation and NMR Analysis Protocol
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Optical rotations were measured in CHCl3 using a Jasco P-1010 digital polarimeter (Tokyo, Japan). 1H and 13C NMR spectra were recorded at 400/100 MHz in CDCl3 on a Bruker spectrometer (AscendTM400) (Bremen, Germany). The same solvent was used as the internal standard. (COSY)-45 experiments were performed using standard Bruker microprograms [21 ]. Analytical and preparative TLC were performed on silica gel plates (Kieselgel 60, F254, 0.25 mm) purchased from Merck (Darmstadt, Germany). The spots were visualized by exposure to UV radiation (253 nm), or by spraying with 10% H2SO4 in MeOH, followed by heating at 110 °C for 10 min. Column chromatography (CC) was performed using silica gel (Kieselgel 60, 0.063–0.200 mm) purchased from Merck.
6
Characterization of Chemical Compounds
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Optical rotations were measured in MeOH on a P-1010 digital polarimeter (Jasco, Tokyo, Japan), unless otherwise noted. IR spectra were recorded as a glass film deposits using a 5700 FT-IR spectrometer (Jasco), and UV spectra were measured in MeCN on a V-530 spectrophotometer (Easton). 1 H and 13 C NMR spectra were recorded, respectively, at 400 and 100 MHz in CDCl 3 , on a Bruker spectrometer (Billerica), using the same solvent as internal standard. The multiplicities were determined by DEPT spectrum (Berger and Braun, 2004) . COSY, HSQC, HMBC and NOESY spectra were recorded using Bruker microprograms. HR ESIMS spectra were recorded on a 6120 Quadrupole LC/MS instrument (Agilent Technologies). Analytical (0.25 mm thickness) and preparative TLC (0.50 mm thickness) were performed on silica gel (Kieselgel 60, F 254 ,) and on reversed phase (Kieselgel 60 RP-18, F 254 , 0.20 mm tickness) plates (Merck). Resulting spots were visualized by exposure to UV radiation (253 nm), or by spraying first with 10% H 2 SO 4 in MeOH and then with 5% phosphomolybdic acid in EtOH, followed by heating at 110°C for 10 min. Column chromatography was performed using silica gel (Merck, Kieselgel 60, 0.063-0.200 mm).
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