1.5% low melting point agarose (Sigma) was prepared to fix the 5-dpf larvae alive without anaesthetics. A dorsal view of the optic tectum is placed. Living imagines were carried out in 1 μm/optical section (40–65 μm in depth) in a 5-min interval using Olympus FV1000 upright confocal microscope (473 nm, 543 nm; Japan) with a Zeiss 40×NA 0.80 water immersion.
Fv1000 upright confocal microscope
Manufactured by Zeiss
Sourced in Japan
The FV1000 upright confocal microscope is a high-performance imaging system designed for advanced microscopy applications. It features a confocal scanning system that enables optical sectioning and high-resolution imaging of samples. The FV1000 is capable of capturing detailed images with excellent contrast and resolution, making it a versatile tool for various research and analysis needs.
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Lab products found in correlation
2 protocols using fv1000 upright confocal microscope
1
Live Imaging of Optic Tectum in Larval Zebrafish
2
Immunofluorescence Imaging of DNA Damage
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Tissue sections or SiHa and A549 cells grown on coverslips were permeabilized in 0.1% Triton and incubated with mouse anti-γH2AX antibody and rabbit antibodies against H3p, β-tubulin, and Chk2T68 (listed in Supplementary Table S2 ). Secondary labeling employed anti-rabbit and anti-mouse antibodies conjugated to Alexa Fluor® 488, 568 or 647, and nuclei were counterstained with DAPI. Images were observed using an Olympus FV1000 upright confocal microscope or Zeiss AxioImager upright microscope and captured at 405 nm, 488 nm, 568 nm and 647 nm using 40x objective lens, before processing with FV10-ASW 3.0 Viewer or AxioVision software.
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