The blood samples were conducted 48 hours before and after intervention (Figure 2 ). Fasting venous blood samples (10 ml) were drawn after supine rest in the morning at the Laboratory of Biochemistry. Samples were immediately centrifuged at 3000 rpm (× g) and 4°C for 15 min. Aliquots of the resulting plasma were stored at -80°C until analysed. All samples were analysed in the same assay run to eliminate inter-assay variance. All assays were performed with Bio-Rad control. BG was measured enzymatically using the hexokinase method. Total cholesterol (TC), HDL, LDL and triglycerides (TG) were measured enzymatically. C-reactive protein (CRP) was measured with the immune-turbidimetric method. T and C concentrations were assessed by chemiluminescent microparticle immunoassay (CMIA). For all the above assays, the COBAS 6000 automated system by Roche Diagnostics (Tokyo, Japan) and all relevant diagnostic reagents of the same company were used.
Cobas 6000 automated system
Manufactured by Roche
Sourced in Japan
The COBAS 6000 automated system is a clinical chemistry and immunochemistry analyzer designed for use in clinical laboratories. It is capable of performing a variety of routine and specialized tests on various sample types, including serum, plasma, and urine. The system is intended to provide accurate and reliable results through its automated processing capabilities.
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Lab products found in correlation
2 protocols using cobas 6000 automated system
1
Blood Sample Collection and Analysis
2
Salivary Cortisol Measurement Protocol
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In the current study, levels of cortisol were measured in saliva. It has been established that salivary levels closely match those found in corresponding blood samples [44 (link)]. Saliva samples were collected 30 min after the child was awakened while keeping the child fasting [45 (link)]. Taking saliva for this analysis required several precautions to avoid any interference that could distort the test results. Parents were informed that their children should not brush their teeth prior to collection. Children had to spit directly into the dry test tubes labelled with the relevant information. The samples were kept at ambient temperature and immediately sent to the biochemistry laboratory of Hedi Chaker University Hospital in Sfax.
The collected saliva samples were centrifuged for 15 min at 3000 revolutions/min, and then the supernatant was taken from the Eppendorf tubes and frozen at −20 °C. On the day of the analysis, the samples were thawed to room temperature (20 to 25 °C) and then vortexed and analyzed. Salivary cortisol was assessed using electrochemiluminescence assays (ECLIA) with the COBAS 6000 automated system produced by Roche Diagnostics (Tokyo, Japan), and all relevant diagnostic reagents from the same company were used. The unit of measurement for cortisol is nanograms per milliliter (ng/mL).
The collected saliva samples were centrifuged for 15 min at 3000 revolutions/min, and then the supernatant was taken from the Eppendorf tubes and frozen at −20 °C. On the day of the analysis, the samples were thawed to room temperature (20 to 25 °C) and then vortexed and analyzed. Salivary cortisol was assessed using electrochemiluminescence assays (ECLIA) with the COBAS 6000 automated system produced by Roche Diagnostics (Tokyo, Japan), and all relevant diagnostic reagents from the same company were used. The unit of measurement for cortisol is nanograms per milliliter (ng/mL).
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