Top10 competent bacteria

Manufactured by Thermo Fisher Scientific

Top10 competent bacteria are a laboratory strain of Escherichia coli (E. coli) bacteria that have been genetically modified to be highly efficient at taking up and incorporating foreign DNA. This makes them a useful tool for cloning and amplifying DNA sequences in various molecular biology applications.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

TOP10 bacteria (10 citations) TOP10 chemically competent bacteria (2 citations)

2 protocols using top10 competent bacteria

Versatile Lentiviral Vector Engineering

Check if the same lab product or an alternative is used in the 5 most similar protocols

All gene expression
constructs were built into the pBOB lentiviral backbone (third generation
lentiviral vector^{15 (link)}). Reporter genes [CBG99
(Green Luc), PRE9 (Red Luc), and mStrawberry (mStraw)], protein degron
sequences, additional genetic elements (e.g., E2A sequence^{16 (link)}), and useful flanking restriction sites were
all synthesized from GenScript USA (New Jersey) and assembled as finished
vectors (as shown in Figures 1 and S1) within 1 to 3 restriction
enzyme or Gibson Assembly cloning steps. All restriction enzymes,
T4 DNA ligase, taq DNA polymerase, and the Gibson cloning kit were
supplied by NEB. All plasmids were grown in Top10 competent bacteria
(Invitrogen, Waltham, MA), and plasmid DNA was prepped from bacteria
with Qiagen DNA mini- or maxi-prep kits (Germantown, MD).

Chung T., Garcia L., Swamynathan M.M., Froeling F.E., Trotman L.C., Tuveson D.A, & Lyons S.K. (2023). Internally Controlled and Dynamic Optical Measures of Functional Tumor Biology. Analytical Chemistry, 95(13), 5661-5670.

+ Open protocol

+ Expand

Murine NIS Expression Construct

Check if the same lab product or an alternative is used in the 5 most similar protocols

The construct (Figure 1) was built into the pBOB lentiviral backbone (3^rd generation lentiviral vector) [36 (link)] to facilitate labeling and stable expression of murine NIS in tumor cell lines of interest. The in vitro selection cassettes (PGK promoter driven TurboGFP-T2A-puromycin resistance) flanked by FRT sites [22 (link)] were synthesized by GenScript USA (New Jersey). We used NCBI Reference Sequence: NP_444478.2 (Supplemental Figure S1) as the consensus coding sequence for murine NIS (Slc5a5). The FRT'd selection cassette and mNIS were cloned into the final construct in two sequential cloning steps. All restriction enzymes, T4 DNA ligase and taq DNA polymerase were supplied by NEB. All cloning and sequencing primers were supplied by Sigma (Burlington, MA). All plasmids were grown up in Top10 competent bacteria (Invitrogen, Waltham, MA) and plasmid DNA prepped from bacteria with Qiagen DNA mini prep or maxi prep kits (Germantown, MD).

Merrill J.R., Inguscio A., Chung T., Demestichas B., Garcia L.A., Habel J., Lewis D.Y., Janowitz T, & Lyons S.K. (2023). Sensitive, non-immunogenic in vivo imaging of cancer metastases and immunotherapy response. Cell Stress, 7(8), 59-68.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!