SEM techniques will be used to visualize and quantitatively analyze the fibrin clot net obtained from plasma. The rinsed clot will be fixed using 2.5% buffered glutaric formaldehyde, rinsed, dehydrated, dried at the critical point, and gold sprayed. The samples will be scanned in 6 different areas (Jeol JCM-6000 microscope) and analyzed with the application of appropriate software Image J (Bethesda, MD, USA) [34 (link)].
Jcm 6000 microscope
Manufactured by JEOL
Sourced in United States
The JCM-6000 is a versatile scanning electron microscope (SEM) designed for high-resolution imaging and analysis of a wide range of samples. It features a high-performance electron optical system, advanced imaging capabilities, and user-friendly software interface. The JCM-6000 is capable of producing detailed images of micro- and nano-scale structures with a resolution up to 3 nanometers.
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Lab products found in correlation
4 protocols using jcm 6000 microscope
1
Scanning Electron Microscopy of Fibrin Clot
2
Scanning Electron Microscopy of Rice Grains
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Freeze-dried GR samples were equilibrated overnight in a desiccator. Samples were fixed on aluminum studs using a double-sided adhesive tape and then were sputter-coated with gold using a TED PELLA Cressington Sputter Coater (TED PELLA, Inc., Redding, CA, USA). The surface microstructure of rice grain samples with or without PEF treatment was observed using a JCM-6000 microscope (JEOL USA, Inc., Pleasanton, CA, USA). The sputter-coated samples were transferred to a microscope mount at an acceleration voltage of 15 kV and observed at a magnification of 500×.
3
Rice Grain Microstructure Analysis
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Freeze dried rice grain samples were equilibrated overnight in a desiccator. Samples were fixed on aluminum studs using a double-sided adhesive tape, and then were sputter-coated with gold using a TED PELLA Cressington Sputter Coater (TED PELLA, Inc., Redding, CA, USA). The surface microstructure of glutinous rice grain samples with or without PEF treatment was observed using a JCM-6000 microscope (JEOL USA, Inc., Pleasanton, CA, USA). The sputter coated samples were transferred to microscope mount at an acceleration voltage of 15 kV, and observed at a magnification of 500×.
4
Mouse Hair Morphology Analysis
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After the mice were dissected, the hair samples of the mice in each group were collected. Five hairs from each site of each mouse were examined. The morphological observation was carried out with the aid of a scanning electron microscope (SEM) in order to detect the differences in the morphology and density (the degree of dispersion of hair) of the hair of the mice in each group. Hair stands were withdrawn from mice using scissors, SEM analyses were performed as described previously [21 (link)] with JEOL JCM-6000 microscope (Tokyo, Japan) operating at an acceleration voltage of 15 kV. For semi-quantitative assessment of the mouse hair dysmorphology, the previously proposed scale of hair morphological changes was used [21 (link)]. In addition, the hair surface microstructure was identified by analyzing the formation of epidermal scales in SEM images [22 (link)].
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