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Anti rabbit and anti mouse conjugated with horse radish peroxidase

Manufactured by Vector Laboratories
Sourced in United States

Anti-rabbit and anti-mouse conjugated with horse-radish peroxidase (HRP) is a secondary antibody reagent used in various immunoassay techniques. The antibody is specific to rabbit or mouse primary antibodies and is conjugated with the enzyme HRP, which can be used for colorimetric or chemiluminescent detection.

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2 protocols using anti rabbit and anti mouse conjugated with horse radish peroxidase

1

Immunohistochemistry for Alzheimer's Markers

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Primary antibodies against pAkt1 (Ser473, Thr308), Akt1, pGSK3β (Ser9), p mTOR (Ser2448), mTOR, pS6K (Thr389), S6K, p4EBP1 (Thr46/47), 4EBP1 and LC3b I/II were purchased from Cell Signaling Technologies, Denvers, MA, USA. Anti-CD31 anti-Iba-1, anti-Abeta and anti NeuN antibodies are from Abcam, and anti-β-tubulin antibody was from Sigma, St. Louis, MO, USA. Tetramethylbenzidine (TMB) substrate kit and secondary antibodies; anti-rabbit and anti-mouse conjugated with horse-radish peroxidase (HRP) were purchased from Vector Laboratories, Burlingame, CA, USA. Endothelin-1 was purchased from Millipore. Isolectin dye was purchased from Life science technologies. All other chemicals and reagents used were of analytical grade and obtained from either Sigma-Aldrich or Merck.
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2

Western Blot Analysis of Signaling Pathways

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Primary antibodies against pAkt1 (Ser473, Thr308), Akt1, pGSK3β (Ser9), p mTOR (Ser2448), mTOR, pS6K (Thr389), S6K, p4EBP1 (Thr46/47) and 4EBP1 were purchased from Cell Signaling Technologies, Denvers, MA, USA. Anti-CD31 and anti-Iba-1 antibodies are from Abcam, and anti-β-tubulin antibody was from Sigma, St. Louis, MO, USA. Tetramethylbenzidine (TMB) substrate kit and secondary antibodies; anti-rabbit and anti-mouse conjugated with horse-radish peroxidase (HRP) were purchased from Vector Laboratories, Burlingame, CA, USA. Endothelin-1 was purchased from Millipore. All other chemicals and reagents used were of analytical grade and obtained from either Sigma-Aldrich or Merck.
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