4 1bb pe

Manufactured by Thermo Fisher Scientific

The 4–1BB-PE is a fluorescently labeled antibody that binds to the 4–1BB receptor expressed on activated T cells. It is designed for use in flow cytometry applications to identify and analyze 4–1BB positive cells.

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Lab products found in correlation

Cd4 pe rpa t4, by BD (1 mentions) Sh800s instrument, by Sony (1 mentions) Facscanto 2, by BD (1 mentions) Efluor 780 live dead stain, by Thermo Fisher Scientific (1 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions) Foxp3 apc, by Thermo Fisher Scientific (1 mentions) Cd8 pe cy7, by BioLegend (1 mentions) Foxp3 staining buffer set, by Thermo Fisher Scientific (1 mentions) Facsaria, by BD (1 mentions) Facsverse, by BD (1 mentions)

2 protocols using 4 1bb pe

Isolation and Analysis of Mouse T Cell Subsets

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The anti-mouse TCRβ-fluorescein isothiocyanate (FITC) was purchased from BioLegend. CD4-PE (RPA-T4), and CD8-PE-Cy7 (SK1) were purchased from BD Biosciences. 4–1BB-PE were purchased from eBioscience. Flow cytometry data acquisition was performed on FACSCanto II (BD Biosciences) followed by the analysis of acquired data by FlowJo software (TreeStar). Co-cultures were sorted based on 4–1BB⁺ separately for CD3⁺CD4⁺CD8⁻ (CD4) and CD3⁺CD4⁻CD8⁺ (CD8) by SH800S instrument (Sony Biotechnology).

Paria B.C., Levin N., Lowery F., Pasetto A., Deniger D.C., Parkhurst M.R., Yossef R., Kim S.P., Florentin M., Ngo L., Ray S., Krishna S., Robbins P.F, & Rosenberg S.A. (2021). Rapid identification and evaluation of neoantigen-reactive T cell receptors from single cells.. Journal of immunotherapy (Hagerstown, Md. : 1997), 44(1), 1-8.

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Ovarian Cancer Immune Cell Profiling

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Before staining with relevant antibodies, cells from ovarian cancer patients were incubated for 10 min with 10mg/mL KIOVIG (Baxalta). Cells were stained with either fixable eFluor780 Live/Dead stain (eBioscience) or aqua live/dead viability stain (Invitrogen) at 4 C in PBS. For cell surface staining antibodies were incubated with cells in the dark for 30 min at 4 C in PBS/1% BSA + 10% FCS (GIBCO). Intracellular staining was with a Foxp3 staining buffer set (eBioscience). Cells were analyzed by flow cytometry using a BD FACSAria or BD FACSVerse. Fluorophore conjugated antibodies against the following cell markers were used: Ovarian samples -CD4-BV510 (RPA-T4), CD25-BV421 (M-A251), CD127-FITC (HIL-7R-M21), CD8-APC (RPA-T8), 41BB-PE (4B4-1), mouse IgG2a isotype,k control-PE (G155-178; all from BD Biosciences); SCC-CD3-APC-Cy7, CD4-FITC or PerCP Cy5.5, CD8-PE Cy7 (all Biolegend), 4-1BB-PE and Foxp3-APC (both eBioscience).

Buchan S.L., Dou L., Remer M., Booth S.G., Dunn S.N., Lai C., Semmrich M., Teige I., Mårtensson L., Penfold C.A., Chan H.T.C., Willoughby J.E., Mockridge C.I., Dahal L.N., Cleary K.L.S., James S., Rogel A., Kannisto P., Jernetz M., Williams E.L., Healy E., Verbeek J.S., Johnson P.W.M., Frendéus B., Cragg M.S., Glennie M.J., Gray J.C., Al-Shamkhani A, & Beers S.A. (2018). Antibodies to Costimulatory Receptor 4-1BB Enhance Anti-tumor Immunity via T Regulatory Cell Depletion and Promotion of CD8 T Cell Effector Function. Immunity, 49(5).

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