Elemental analysis of C, H, and N was performed with a model EuroVector EA3000 (for imidazo [1,2-a]pyridine derivatives) and Perkin-Elmer 240 (for complex) elemental analyzer. The LC-MS spectra were recorded using Thermo scientific mass spectrophotometer (USA). Bruker Avance spectrophotometer was used for recording 1H NMR (400 MHz) and 13C NMR (100 MHz) spectra. Melting points were determined in open capillaries on hermocal10 melting point apparatus (Analab Scientific Pvt. Ltd, India). The Gouy's method was used for magnetic measurement of metal complexes taking mercury tetrathiocyanatocobaltate (II) as the calibrant (χg = 16. 44 × 10-6 cgs units at 20 °C), citizen balance. UV-160A UV-Vis spectrophotometer, Shimadzu, Kyoto (Japan), was used for electronic spectra of metal complexes in the range of 200–800 nm using quarts cell having a path length of 1 cm. Ubbelohde viscometer in viscosity bath having a controllable temperature (25.0 ± 0.5 °C) was used for the study of hydrodynamic chain length. The antibacterial study was carried out by means of laminar air flow cabinet Toshiba, Delhi (India). Alphadigidoctm RT. Version V.4.0.0 PC– Image software, CA (USA) used for the Photo quantization of the DNA cleavage activity.
Uv 160a uv vis spectrophotometer
Manufactured by Shimadzu
Sourced in Japan
The UV-160A UV-Vis spectrophotometer is a laboratory instrument designed to measure the absorbance or transmittance of a sample across the ultraviolet and visible light spectrum. It is capable of analyzing a wide range of liquid and solid samples. The UV-160A provides accurate and reliable measurements for various applications in scientific research and analysis.
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3 protocols using uv 160a uv vis spectrophotometer
1
Characterization of Imidazo[1,2-a]pyridine Derivatives
2
Colorimetric Determination of Total Carbohydrates in Mucin
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Total carbohydrates in the mucin sample were colorimetrically determined by m-hydroxydiphenyl method. Firstly, a stock solution of glucose (200 mg/L) was prepared and from this stock solution, standard solutions with concentrations of 0 -100 mg/L were prepared, then the glucose test was performed by taking 400 µL from the standard solutions. Two ml of 0.5 % borax in concentrated sulphuric acid was added and then incubated at 100 o C in water bath for 5 min to which 40 µL of 0.15 % 3-phenylphenol (in 1 M sodium hydroxide) was added and incubated for 5 min. The absorbance for each standard and the sample was measured at 520 nm using Shimadzu UV-160A UV-vis spectrophotometer. The blank for the sample was prepared by taking 400µL of the sample, 2 mL of deionised water and 40 µL of 0.15 % 3-phenylphenol while the blank for the standard was prepared by taking 400 µL of deionised water, 2 mL of 0.5 % borax in concentrated sulphuric acid and 40 µL of 0.15 % 3-phenylphenol.
3
Spectroscopic Characterization and Antimicrobial Evaluation
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The 1 H and 13 (link) C NMR spectra were recorded with a Bruker Avance (400 MHz) spectrometer using deuterated dimethyl sulfoxide and deuterated chloroform solvent. FT-IR Shimadzu spectrophotometer was used for IR spectra in the range 4000-300 cm -1 . C, H, and N elemental analysis were performed with a model Perkin-Elmer 240 elemental analyser. Melting points were determined in open capillaries on hermoCal10 melting point apparatus (Analab Scientific Pvt. Ltd, India). The LC-MS spectra were recorded using Thermo scientific mass spectrometer (USA). The electronic spectra of metal complexes were recorded on a UV-160A UV-Vis spectrophotometer, Shimadzu, Kyoto (Japan). The Gouy's method used for magnetic measurement of metal complexes taking mercury tetrathiocyanatocobaltate(II) as the calibrant (χ g = 16.44 • 10 -6 cgs units at 20 °C), citizen balance. Antibacterial study was carried out by means of laminar air flow cabinet Toshiba, Delhi (India). AlphaDigiDoc TM RT. Version V.4.0.0 PC-Image software, CA (USA) used for the Photo quantization of the DNA cleavage activity.
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