Particle size and concentration distribution of the EMNs and exosomes were also measured using NTA (v2.3; Malvern Instruments, Malvern, UK) according to manufacturer’s instructions. Briefly, EMNs samples were vortexed and diluted to a final dilution of 1:1000 in milliQ H2O and exosomes 1:100. Blank-filtered H2O was run as a negative control. Each sample analysis was conducted for 60 s and measured five times using Nanosight automatic analysis settings. The detection threshold was set to level 11 and camera level to 15.
Nta v2.3
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The NTA (v2.3) is a particle analysis instrument manufactured by Malvern Panalytical. It utilizes Nanoparticle Tracking Analysis (NTA) technology to measure the size, concentration, and movement of nanoparticles in liquid suspensions.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using nta v2.3
1
Nanoparticle size and concentration analysis
2
Characterizing BMSC-Derived Exosomes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Particle size and concentration distribution of the isolated exosomes stemmed from BMSCs were measured using NTA (v2.3; Malvern Instruments, Malvern, UK) according to manufacturer's instructions. Brie y, exosomes samples were vortexed and diluted to a nal dilution of 1 : 2000 in ltered moleculargrade H2O. Blank-ltered H2O was run as a negative control. Each sample analysis was conducted for 60 s and measured three times using Nanosight automatic analysis settings.
Transmission electron microscopy (TEM)
TEM analysis was performed to con rm BMSCs-derived exosomes morphology. Brie y, exosomes samples were diluted with PBS to the appropriate concentration, and ~20-40 μL of PBS solution containing exosomes was transferred to a copper grid for incubation at room temperature for 5 min. Filter paper was used for absorbing unevaporated solution. Exosomes samples were negatively stained with 4% paraformaldehyde acid solution at room temperature for 5 min and dried at 65 °C for 10 min. Images of exosomes samples were obtained using a Hitachi H-7650 transmission electron microscope (Hitachi, Tokyo, Japan).
Transmission electron microscopy (TEM)
TEM analysis was performed to con rm BMSCs-derived exosomes morphology. Brie y, exosomes samples were diluted with PBS to the appropriate concentration, and ~20-40 μL of PBS solution containing exosomes was transferred to a copper grid for incubation at room temperature for 5 min. Filter paper was used for absorbing unevaporated solution. Exosomes samples were negatively stained with 4% paraformaldehyde acid solution at room temperature for 5 min and dried at 65 °C for 10 min. Images of exosomes samples were obtained using a Hitachi H-7650 transmission electron microscope (Hitachi, Tokyo, Japan).
3
Characterizing BMSC-Derived Exosomes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Particle size and concentration distribution of the isolated exosomes stemmed from BMSCs were measured using NTA (v2.3; Malvern Instruments, Malvern, UK) according to manufacturer's instructions. Brie y, exosomes samples were vortexed and diluted to a nal dilution of 1 : 2000 in ltered moleculargrade H2O. Blank-ltered H2O was run as a negative control. Each sample analysis was conducted for 60 s and measured three times using Nanosight automatic analysis settings.
Transmission electron microscopy (TEM)
TEM analysis was performed to con rm BMSCs-derived exosomes morphology. Brie y, exosomes samples were diluted with PBS to the appropriate concentration, and ~20-40 μL of PBS solution containing exosomes was transferred to a copper grid for incubation at room temperature for 5 min. Filter paper was used for absorbing unevaporated solution. Exosomes samples were negatively stained with 4% paraformaldehyde acid solution at room temperature for 5 min and dried at 65 °C for 10 min. Images of exosomes samples were obtained using a Hitachi H-7650 transmission electron microscope (Hitachi, Tokyo, Japan).
Transmission electron microscopy (TEM)
TEM analysis was performed to con rm BMSCs-derived exosomes morphology. Brie y, exosomes samples were diluted with PBS to the appropriate concentration, and ~20-40 μL of PBS solution containing exosomes was transferred to a copper grid for incubation at room temperature for 5 min. Filter paper was used for absorbing unevaporated solution. Exosomes samples were negatively stained with 4% paraformaldehyde acid solution at room temperature for 5 min and dried at 65 °C for 10 min. Images of exosomes samples were obtained using a Hitachi H-7650 transmission electron microscope (Hitachi, Tokyo, Japan).
4
Characterizing BMSC-Derived Exosomes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Particle size and concentration distribution of the isolated exosomes stemmed from BMSCs were measured using NTA (v2.3; Malvern Instruments, Malvern, UK) according to manufacturer's instructions. Brie y, exosomes samples were vortexed and diluted to a nal dilution of 1 : 2000 in ltered moleculargrade H2O. Blank-ltered H2O was run as a negative control. Each sample analysis was conducted for 60 s and measured three times using Nanosight automatic analysis settings.
Transmission electron microscopy (TEM)
TEM analysis was performed to con rm BMSCs-derived exosomes morphology. Brie y, exosomes samples were diluted with PBS to the appropriate concentration, and ~20-40 μL of PBS solution containing exosomes was transferred to a copper grid for incubation at room temperature for 5 min. Filter paper was used for absorbing unevaporated solution. Exosomes samples were negatively stained with 4% paraformaldehyde acid solution at room temperature for 5 min and dried at 65 °C for 10 min. Images of exosomes samples were obtained using a Hitachi H-7650 transmission electron microscope (Hitachi, Tokyo, Japan).
Transmission electron microscopy (TEM)
TEM analysis was performed to con rm BMSCs-derived exosomes morphology. Brie y, exosomes samples were diluted with PBS to the appropriate concentration, and ~20-40 μL of PBS solution containing exosomes was transferred to a copper grid for incubation at room temperature for 5 min. Filter paper was used for absorbing unevaporated solution. Exosomes samples were negatively stained with 4% paraformaldehyde acid solution at room temperature for 5 min and dried at 65 °C for 10 min. Images of exosomes samples were obtained using a Hitachi H-7650 transmission electron microscope (Hitachi, Tokyo, Japan).
5
Characterizing BMSC-Derived Exosomes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Particle size and concentration distribution of the isolated exosomes stemmed from BMSCs were measured using NTA (v2.3; Malvern Instruments, Malvern, UK) according to manufacturer's instructions. Brie y, exosomes samples were vortexed and diluted to a nal dilution of 1 : 2000 in ltered moleculargrade H2O. Blank-ltered H2O was run as a negative control. Each sample analysis was conducted for 60 s and measured three times using Nanosight automatic analysis settings.
Transmission electron microscopy (TEM)
TEM analysis was performed to con rm BMSCs-derived exosomes morphology. Brie y, exosomes samples were diluted with PBS to the appropriate concentration, and ~20-40 μL of PBS solution containing exosomes was transferred to a copper grid for incubation at room temperature for 5 min. Filter paper was used for absorbing unevaporated solution. Exosomes samples were negatively stained with 4% paraformaldehyde acid solution at room temperature for 5 min and dried at 65 °C for 10 min. Images of exosomes samples were obtained using a Hitachi H-7650 transmission electron microscope (Hitachi, Tokyo, Japan).
Transmission electron microscopy (TEM)
TEM analysis was performed to con rm BMSCs-derived exosomes morphology. Brie y, exosomes samples were diluted with PBS to the appropriate concentration, and ~20-40 μL of PBS solution containing exosomes was transferred to a copper grid for incubation at room temperature for 5 min. Filter paper was used for absorbing unevaporated solution. Exosomes samples were negatively stained with 4% paraformaldehyde acid solution at room temperature for 5 min and dried at 65 °C for 10 min. Images of exosomes samples were obtained using a Hitachi H-7650 transmission electron microscope (Hitachi, Tokyo, Japan).
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