Changes in cell morphology and shape induced by different compounds represent specific signs of cytotoxicity. Therefore, the effect of the tested compounds on HT-29 cell morphology was evaluated as a part of the toxicological profile. The cells were photographed under bright field illumination of the Olympus IX73 inverted microscope (Olympus) at 24 h post-stimulation with the test compounds (CdCl2 or CuSO4, 0.05; 0.2; 1; 10 and 100 µg/ml) and the photos were analyzed by the cellSens Dimensions v.1.8. software (Olympus).
Cellsens dimensions v 1
Manufactured by Olympus
Sourced in Japan
CellSens Dimensions v.1.8 Software is a data acquisition and analysis software designed for microscopy imaging. It provides tools for image capture, processing, and analysis.
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Lab products found in correlation
3 protocols using cellsens dimensions v 1
1
Assessing Cytotoxicity through Cell Morphology
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Hoechst 33342 Apoptosis Assay in HaCaT and SAOS-2 Cells
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To verify the effect induced by test samples (P1, P2, P3 and P4) at the nuclear level in HaCaT and SAOS-2 cells, Hoechst 33342 staining was employed by using a working solution of 1:2000 in PBS. The staining was realized 24 h post-exposure to test samples. To observe the nuclear changes that occurred after exposure to test samples, an Olympus IX73 inverted microscope (Olympus, Tokyo, Japan) was used, and pictures were taken under UV irradiation using the integrated DP74 digital camera. Further, the images obtained were analyzed using cellSens Dimensions v.1.8 Software (Olympus, Tokyo, Japan), and the apoptotic index was determined by quantifying the apoptotic cells relative to the total number of cells.
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Cytotoxicity Evaluation of Cell Lines
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The cell morphology of HaCaT and SAOS-2 cells was evaluated after 24 h of stimulation to assess the compounds’ cytotoxic potential. The impact on cellular morphology was evaluated by photographing the cells under bright field illumination using an Olympus IX73 inverted microscope (Olympus, Tokyo, Japan). Finally, the images were analyzed using cellSens Dimensions v.1.8 Software (Olympus, Tokyo, Japan).
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