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Fip200 shrna

Manufactured by Thermo Fisher Scientific

The FIP200 shRNA is a laboratory tool used for gene knockdown experiments. It is a short hairpin RNA (shRNA) designed to target and reduce the expression of the FIP200 gene. The FIP200 gene is involved in various cellular processes, and its knockdown can be used to study its function. The FIP200 shRNA can be used in cell culture systems to investigate the role of the FIP200 gene in cellular pathways and phenotypes.

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2 protocols using fip200 shrna

1

Lentiviral shRNA Knockdown Protocol

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Human scrambled shRNA, FIP200 shRNA, and Tsc1 shRNA encoded in pGIPZ lentiviral vector were purchased from Open Biosystems (Lafayette, CO). Mouse scrambled shRNA, LAL shRNA #1 and #2 were purchased from Sigma. Package vectors of pSPAX2 and pMD2.G were mixed with shRNA vectors and transfected in HK293 cells for virus production essentially as described before37 (link).
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2

Lentiviral shRNA Knockdown Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human scrambled shRNA, FIP200 shRNA, and Tsc1 shRNA encoded in pGIPZ lentiviral vector were purchased from Open Biosystems (Lafayette, CO). Mouse scrambled shRNA, LAL shRNA #1 and #2 were purchased from Sigma. Package vectors of pSPAX2 and pMD2.G were mixed with shRNA vectors and transfected in HK293 cells for virus production essentially as described before37 (link).
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