Hrp conjugated donkey antibody to rabbit igg

The following primary antibodies were used in this study: rabbit antibody to AIMP2 (1: 2000, ProteinTech Group, Rosemont, IL, USA), goat antibody to VPS35 (1:2000, Abcam, Cambridge, MA, USA), rabbit antibody to Lamin A/C (1:1000, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit antibody to α-tubulin-1 (1:1000, Cell Signaling Technology, Danvers, MA, USA), mouse antibody to Lamp2a (1:1000, Abcam), mouse antibody to PAR (1:1000, Trevigen, Gaithersburg, MD, USA), mouse antibody to PARkin (Park8, 1:2000, Cell Signaling), mouse antibody to FLAG (M2, 1:5000, Sigma-Aldrich, St. Louis, MO, USA), mouse antibody to V5 (1:500, Invitrogen), horseradish peroxidase (HRP)-conjugated antibody to V5 (1:5000, Invitrogen), HRP-conjugated antibody to FLAG (1:5000, Invitrogen, Carlsbad, CA, USA), HRP-conjugated mouse antibody to β-actin (AC15, 1:20 000, Sigma-Aldrich). The following secondary antibodies were used: HRP-conjugated antibody to mouse IgG (1:5000, GE Healthcare, Pittsburgh, PA, USA), HRP-conjugated donkey antibody to rabbit IgG (1:5000, GE Healthcare), HRP-conjugated rabbit antibody to goat IgG (1:3000, Sigma-Aldrich), Alexa-488 conjugated antibody to rabbit IgG (H+L) (1:250, Thermo Fisher Scientific, Waltham, MA, USA), Alexa-568 conjugated antibody to goat IgG (H+L) (1:250, Thermo Fisher Scientific), Alexa-647-conjugated antibody to mouse IgG (H+L) (1:250, Thermo Fisher Scientific).

Rhododendrin was provided by the National Development Institute of Korean Medicine (NIKOM, Gyeongsan, Korea). Rhododendrin was purified from Rhododendron brachycarpum, and validated via high-performance liquid chromatography by NIKOM. 6-OHDA was purchased from Sigma. Tamoxifen was purchased from Selleck Chemicals (Houston, TX, USA).
The following primary antibodies were used: mouse antibody to RNF146 (N201/35, 1:5000, NeuroMab, Davis, CA, USA), mouse antibody to PAR (cat# 4335-MC-100, 1:3000, Trevigen, Gaithersburg, MD, USA), rabbit antibody to ERβ (cat#PA1-311, 1:3000, Invitrogen), rabbit antibody to tyrosine hydroxylase (NB300-109, 1:2000, Novus Biologicals, Centennial, CO, USA). For secondary antibodies, we used horse radish peroxidase (HRP)-conjugated sheep antibody to mouse IgG (cat# RPN4301, 1:5000, GE Healthcare, Pittsburgh, PA, USA), HRP-conjugated donkey antibody to rabbit IgG (cat# RPN4101, 1:5000, GE Healthcare), biotin-conjugated goat antibody to rabbit IgG (cat# BA-1000, 1:1000, Vector Laboratories, Burlingame, CA, USA), and HRP-conjugated mouse antibody to β-actin (cat# A3854, 1:10000, Sigma-Aldrich, St. Louis, MO, USA).