Quercetin (Batch No. Z100081) was purchased from the National Institute for Food and Drug Control (Beijing, China). The leaves of Cacumen Platycladi were purchased from Nanjing Tongrentang (Nanjing, China). Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), and trypsin were purchased from BasalMedia (Shanghai, China). LPS and ATP were purchased from Sigma-Aldrich.
Trypsin
Manufactured by BasalMedia
Sourced in China, United States
Trypsin is a proteolytic enzyme that cleaves peptide bonds on the carboxyl side of lysine and arginine residues. It is commonly used in cell biology and biochemistry applications to dissociate cells and tissues for culturing, protein extraction, and other laboratory procedures.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by BasalMedia (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Dulbecco s modified eagle medium dmem, by BasalMedia (1 mentions)
Cell counting kit 8 kit, by Beyotime (1 mentions)
96 well plate, by Corning (1 mentions)
Pen strep, by Thermo Fisher Scientific (1 mentions)
Non essential amino acid (neaa), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
β mercaptoethanol, by Thermo Fisher Scientific (1 mentions)
3 protocols using trypsin
1
Quercetin Extraction and Cell Culture
2
Gastric Cancer Cell Proliferation Assay
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Gastric cancer cells MKN74, transfected with CBX4-siRNA and negative control siRNAs for 48 h, were detached using trypsin (S330JV, Shanghai Basal Media Technologies Co. LTD) and incubated in a 96-well plate (Corning Inc, New York, USA) at a density of 2 × 103 cells in 100 μl. After culturing for 48 h, the supernatant was removed and cell growth was detected using Cell Counting Kit-8 Kit (C0037, Beyotime, China) according to the manufacturer's instructions. Absorbance was measured at 450 nm using a microplate reader. All experiments were performed in triplicate and repeated at least three times.
3
Culturing Mouse Embryonic Stem Cells
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Mouse embryonic stem cell line CGR8.8 cells (generously provided by Dr Yanru Chen from Stanford University) were cultured in an ESCs culture medium50 that consists of 15% FCS (PAA), 1x non‐essential amino acid (Gibco), 1% Pen/Strep (Gibco), 4 mM L‐glutamine (Gibco), 0.1 mM β‐mercaptoethanol (Gibco), 1000 U/ml LIF (Millipore), and DMEM (Gibco).
EB formation was used as a quick functional assay to predict the cell fate of the three germ layers in vitro. CGR8.8 cells were allowed to differentiate when cultured in ESCs culture medium without LIF. EB morphology was observed on Day 2 through Day 5. EBs were treated with 0.25% trypsin (BasalMedia) to dissociate individual cells and then subjected to flow cytometry analysis.
EB formation was used as a quick functional assay to predict the cell fate of the three germ layers in vitro. CGR8.8 cells were allowed to differentiate when cultured in ESCs culture medium without LIF. EB morphology was observed on Day 2 through Day 5. EBs were treated with 0.25% trypsin (BasalMedia) to dissociate individual cells and then subjected to flow cytometry analysis.
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