Empyrean xrd

The nanoparticles were biosynthesized by adding 6.0 ml of silver nitrate solution (1 M) with 40 ml of extract and 154 ml of deionized water. The reaction mixture was observed for color change depending on parameter studied such as time, silver nitrate, and extract concentration at 80°C. The resultant reddish brown-colored reaction mixture was then centrifuged at 12,000 rpm for 10 min (REMI, India). The pellet obtained was washed thrice with deionized water and finally with acetone. The resultant pellet was dried and stored for further characterizations. The preparation of silver nanoparticles was characterized by ultraviolet-visible (UV-Vis) spectrophotometer (Themoscientific SpectraScan) in the wavelength range of 340–900 nm. The surface morphology and particle size distribution of the S. podophyllum nanoparticles were examined using an atomic force microscope (AFM, NDMDT) with a conducting P (n)-doped silicon tip under normal atmospheric condition in semicontact mode. The X-ray diffraction (XRD) patterns of silver nanoparticles were obtained using X-ray diffractometer (PANalytical Empyrean XRD) with diffraction angle in the range of 20°–80°.