Cd163 clone 10d6

Manufactured by Novus Biologicals

Sourced in Denmark

CD163 is a cell surface glycoprotein that functions as a scavenger receptor. The clone 10D6 is a monoclonal antibody that recognizes the CD163 antigen. This product is intended for research use only.

Automatically generated - may contain errors

Lab products found in correlation

Ventana benchmark stainer, by Roche (1 mentions) Ultraview universal dab detection kit, by Roche (1 mentions) Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CD163 (4 citations)

2 protocols using cd163 clone 10d6

Tissue Microarray Construction and Immunohistochemical Analysis

Cited 2 times

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Tissue microarrays (TMA) were constructed as previously described [22 (link), 23 (link)], using a semi-automated arraying device (TMArrayer, Pathology Devices, Westminister, MD, USA). A set of three 1 mm cores was obtained from viable, non-necrotic areas of the primary tumours. For immunohistochemical (IHC) analysis of CD1a, CD68, CD163 and MARCO, 4 μm TMA-sections were pre- treated using ULTRA Cell Conditioning Solution 1, pH 8.5 (Ventana Medical Systems Inc., Tucson, AZ, USA) for heat induced epitope retrieval, and stained in a Ventana BenchMark stainer (Ventana Medical Systems Inc.) with the following antibodies: CD1a: clone NCL-CD1a-220, diluted 1:25, LEICA Biosystems, Newcastle, UK, CD68: clone KP1, diluted 1:1000, Dako, Glostrup, Denmark, CD163: clone 10D6 diluted 1:200 Novus Biologicals, Abingdon, United Kingdom, MARCO clone HPA063793, diluted 1:250, Atlas Antibodies, Bromma, Sweden. The antibody-antigen complex was visualized with ultraView Universal DAB Detection kit (Ventana Medical Systems Inc.).

Lundgren S., Karnevi E., Elebro J., Nodin B., Karlsson M.C., Eberhard J., Leandersson K, & Jirström K. (2017). The clinical importance of tumour-infiltrating macrophages and dendritic cells in periampullary adenocarcinoma differs by morphological subtype. Journal of Translational Medicine, 15, 152.

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Multiplex Immunofluorescence Staining of FFPE Tumor

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Sections of FFPE tumour tissues were incubated with primary antibodies for PD-L1 and CD3 (clone SP34-2; Pharmingen), CD163 (clone 10D6; Novus Biologicals), CD11c (clone EP1347Y; Novus Biologics), or cytokeratin (CK; clone 5D3/LP34, Abcam) at room temperature. Detection was performed using Novocastra PowerVision Poly-HRP IHC Detection Systems (Leica) followed by Alexa Fluor 594 Tyramide Signal Amplification (TSA) Kit 25 or Alexa Fluor 488 TSA Kit 22 (Life Technologies) according to the manufacturers’ instructions. Slides were mounted in ProLong Gold Antifade Reagent with DAPI (Life Technologies).

Herbst R.S., Soria J.C., Kowanetz M., Fine G.D., Hamid O., Gordon M.S., Sosman J.A., McDermott D.F., Powderly J.D., Gettinger S.N., Kohrt H.E., Horn L., Lawrence D.P., Rost S., Leabman M., Xiao Y., Mokatrin A., Koeppen H., Hegde P.S., Mellman I., Chen D.S, & Hodi F.S. (2014). Predictive correlates of response to the anti-PD-L1 antibody MPDL3280A in cancer patients. Nature, 515(7528), 563-567.

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