Log In Sign up
The largest database of trusted experimental protocols

Complete dmem medium

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States, China, United Kingdom

Complete DMEM medium is a cell culture medium formulation that provides a comprehensive set of nutrients and components required for the growth and maintenance of a wide range of cell types in vitro. It is a widely used basal medium that supports the basic metabolic needs of cells.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (32 mentions) Penicillin, by Thermo Fisher Scientific (16 mentions) Streptomycin, by Thermo Fisher Scientific (16 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (15 mentions) Complete rpmi 1640 medium, by Thermo Fisher Scientific (8 mentions) L glutamine, by Thermo Fisher Scientific (7 mentions) Fetal bovine serum (fbs), by Merck Group (6 mentions) Glutamax, by Thermo Fisher Scientific (6 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (5 mentions) Penicillin streptomycin, by Merck Group (5 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (4 mentions) Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (4 mentions) Mda mb 231, by American Type Culture Collection (4 mentions) Dmem medium, by Thermo Fisher Scientific (3 mentions) Complete dmem f12 medium, by Thermo Fisher Scientific (3 mentions) Mcf 7, by American Type Culture Collection (3 mentions) β mercaptoethanol, by Thermo Fisher Scientific (3 mentions) Huvecs, by Thermo Fisher Scientific (3 mentions) M7145, by Merck Group (3 mentions) Rmpi medium, by Thermo Fisher Scientific (3 mentions)

Close spelling variants of this product

Complete Dulbecco’s modified Eagle’s medium (DMEM; (4 citations) Complete DMEM growth medium (2 citations) DMEM/F-12 complete growth medium (2 citations) DMEM/F-12 ‘complete’ culture medium (5 citations) L-DMEM complete medium (3 citations) Complete DMEM/F12 medium (34 citations) Complete Dulbecco’s modified Eagle medium (DMEM) (5 citations) DMEM high-glucose complete medium (9 citations) Complete DMEM/F12 culture medium (2 citations) Complete DMEM (175 citations)

136 protocols using complete dmem medium

1

Transient Transfection of COS-7 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
COS-7 cells were cultured in DMEM complete medium (Gibco) supplemented with 10% fetal bovine serum and maintained at 37°C in a humidified incubator (Thermo). They were then transiently transfected using LipofectamineTM 2000 (Invitrogen) in accordance with the manufacturer’s protocol when they reached 80% confluence. Before fixation, the cells were grown in DMEM complete medium (Gibco) for 24 h. The cells were then sub-cultured on coverslips (Fisher Scientific) for another 24 h and fixed with 3% (w/v) paraformaldehyde and 0.5% glutaraldehyde in PBS for 15 min at 37°C, washed 3 times with filtered PBS and stored in PBS until imaging.
Xu F., Zhang M., Liu Z., Xu P, & Zhang F. (2015). Bayesian localization microscopy based on intensity distribution of fluorophores. Protein & Cell, 6(3), 211-220.
+ Open protocol
+ Expand
2

Lentiviral Overexpression of miRNA-494 in Rat Microglia

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rat microglia (Fame Biotechnology Co., Ltd., Shanghai, China) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) complete medium (Gibco, Carlsbad, California, USA). A miRNA-494 lentiviral expression vector (Jikai Gene, Shanghai, China) was constructed and transfected into microglia with PLVX-mCMV-ZsGreen-PGK-Puro-homo-miRNA-494 virus multiplicity of infection (MOI) = 20, and a negative control group (NC group) was set up. After the addition of lentivirus, the cells were incubated in a 37 ℃, 5% CO2 incubator (Likang Bio-Medical Technology Holding Co., Ltd., Hong Kong, China) for 16 h. Fresh DMEM complete medium (Gibco) was replaced for 72 h before harvesting the cells.
He C., Aziguli A., Zhen J., Jiao A., Liao H., Du C., Liu W., Aihemaitijiang K, & Xu A. (2022). MiRNA-494 specifically inhibits SIRT3-mediated microglia activation in sepsis-associated encephalopathy. Translational Cancer Research, 11(7), 2299-2309.
+ Open protocol
+ Expand
3

Protein Expression Analysis in Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK 293T and HeLa cells were obtained from ATCC, and grown in DMEM complete medium (Gibco) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals). T47D cells were also obtained from ATCC and grown in RMPI-1640 medium with 10% FBS. RPE1-hTRET (FRT) cells, a kind gift from Peter Jackson, were grown in DMEM complete medium (Gibco) supplemented with 10% FBS. The APC/C inhibitor proTame was purchased from Boston Biochem.
All transfection experiments were performed in HEK 293T. Transfections were performed using Lipofectamine 2000 (Life Technologies). Cells that had been transfected with protein expression vectors were cultured for 24-48h prior to analysis. Samples for protein analysis by immunoblot were lysed in NETN buffer [20 mM Tris-Cl (pH 8.0), 100 mM NaCl, 0.5 mM EDTA and 0.5 % (v/v) Nonidet P-40 (NP-40)] supplemented with 2µg/ml pepstatin, 2µg/ml apoprotinin, 10µg/ml leupeptin, 1mM AEBSF [4-(2 Aminoethyl) benzenesulfonyl fluoride], 1mM Na3VO4, and 1mM NaF. Lysis was performed on ice for ~20 minutes. Protein concentration was determined using Bradford (Bio-Rad). Standard immunoblotting procedures were followed. Membranes were blocked in 5% nonfat dried milk [diluted in Phosphate buffered saline 0.05% tween-20 (PBST)].
RNA interference and Crispr/Cas9 knockout reagents, including oligonucleotide sequences, are described in the supplemental materials and methods.
Choudhury R., Bonacci T., Arceci A., Lahiri D., Mills C.A., Kernan J.L., Branigan T.B., DeCaprio J.A., Burke D.J, & Emanuele M.J. (2016). APC/C and SCFCyclin F constitute a reciprocal feedback circuit controlling S-phase entry. Cell reports, 16(12), 3359-3372.
+ Open protocol
+ Expand
4

Cell Line Culturing and Maintenance

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tu167 cells were obtained from the University of Texas MD Anderson Cancer Center (Houston, TX, USA). All cell lines used in this study were grown in complete DMEM medium (Invitrogen) supplemented with 10% fetal bovine serum (Sigma), 2 mM Gluta-MAX, penicillin (100 U/mL), streptomycin (100 µg/mL) and were tested free of mycoplasma.
Portney B.A., Khatri R., Meltzer W.A., Mariano J.M, & Zalzman M. (2018). ZSCAN4 is negatively regulated by the ubiquitin-proteasome system and the E3 ubiquitin ligase RNF20. Biochemical and biophysical research communications, 498(1), 72-78.
+ Open protocol
+ Expand
5

Cell Line Authentication and Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
HNSCC cell lines were authenticated and tested free of mycoplasma by the University of Maryland translational core facility. All cell lines were cultured in complete DMEM medium (Invitrogen) supplemented with 10% fetal bovine serum (Atlanta Biologicals), 2mM GlutaMAX, penicillin (100U/mL), and streptomycin (100μg/mL) (ThermoFisher Scientific).
Portney B.A., Arad M., Gupta A., Brown R.A., Khatri R., Lin P.N., Hebert A.M., Angster K.H., Silipino L.E., Meltzer W.A., Taylor R.J, & Zalzman M. (2020). ZSCAN4 facilitates chromatin remodeling and promotes the cancer stem cell phenotype. Oncogene, 39(26), 4970-4982.
+ Open protocol
+ Expand
6

Generating Cisplatin-Resistant ACRP Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pan-resistant ACRP cells were generated from its parental counterpart A2780 lineage, following chronic exposure to cisplatin (1μM to 10 μM). Chemoresistance was verified by the MTT method [51 ] through the calculation of estimated IC50 of cisplatin, paclitaxel and doxorubicin. Lineages were cultured in complete DMEM medium (Invitrogen) supplemented with FBS 10%(v/v), penicillin/streptomycin1%(w/v), amphotericin1%(w/v), at 37° C in atmosphere of 5% CO2.
Henriques T.B., dos Santos D.Z., dos Santos Guimarães I., Tessarollo N.G., Lyra-Junior P.C., Mesquita P., Pádua D., Amaral A.L., Cavadas B., Pereira L., Silva I.V., Almeida R.M, & Rangel L.B. (2021). Inhibition of CXCR2 plays a pivotal role in re-sensitizing ovarian cancer to cisplatin treatment. Aging (Albany NY), 13(10), 13405-13420.
+ Open protocol
+ Expand
7

Cultivation of As4.1 Renal Tumor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
As4.1 cells (ATCC, Manassas, VA, USA), an immortalized renin-containing mouse renal tumor cell line, were used in this study. Cells were cultured in complete DMEM medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (Invitrogen), 1 IU/mL penicillin (Invitrogen), and 100 µg/mL streptomycin (Invitrogen).
Yu Z., Yang J., Huang W.J., Zhang T., Li X.M., Zhao W., Li X.Y, & Lu Y.C. (2022). Follicle stimulating hormone promotes production of renin through its receptor in juxtaglomerular cells of kidney. Diabetology & Metabolic Syndrome, 14, 65.
+ Open protocol
+ Expand
8

Tumor Sphere Formation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells (500 per well) were seeded into 6-well, ultralow attachment cluster plates (Corning, NY, USA) and cultured in complete DMEM medium (Invitrogen, Carlsbad, CA, USA). After one week, cells were photographed and counted.
Li L., He Z., Zhu C., Chen S., Yang Z., Xu J., Bi N., Yu C, & Sun C. (2020). MiR-137 promotes anoikis through modulating the AKT signaling pathways in Pancreatic Cancer. Journal of Cancer, 11(21), 6277-6285.
+ Open protocol
+ Expand
9

Mycoplasma-free HNSCC cell culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
HNSCC cell lines were authenticated and tested free of mycoplasma by the University of Maryland translational core facility. All cell lines were cultured in complete DMEM medium (Invitrogen) supplemented with 10% fetal bovine serum (Atlanta Biologicals), 2 mM GlutaMAX, penicillin (100 U/mL), and streptomycin (100 μg/mL) (ThermoFisher Scientific).
Portney B.A., Arad M., Gupta A., Brown R.A., Khatri R., Lin P.N., Hebert A.M., Angster K.H., Silipino L.E., Meltzer W.A., Taylor R.J, & Zalzman M. (2020). ZSCAN4 facilitates chromatin remodeling and promotes the cancer stem cell phenotype. Oncogene, 39(26), 4970-4982.
+ Open protocol
+ Expand
10

Cell Line Culturing and Maintenance

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tu167 cells were obtained from the University of Texas MD Anderson Cancer Center (Houston, TX, USA). All cell lines used in this study were grown in complete DMEM medium (Invitrogen) supplemented with 10% fetal bovine serum (Sigma), 2 mM Gluta-MAX, penicillin (100 U/mL), streptomycin (100 µg/mL) and were tested free of mycoplasma.
Portney B.A., Khatri R., Meltzer W.A., Mariano J.M, & Zalzman M. (2018). ZSCAN4 is negatively regulated by the ubiquitin-proteasome system and the E3 ubiquitin ligase RNF20. Biochemical and biophysical research communications, 498(1), 72-78.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!