Log In Sign up
The largest database of trusted experimental protocols

Pro light hrp chemiluminescent kit

Manufactured by Tiangen Biotech
Visit Supplier
Sourced in China

The Pro-light HRP Chemiluminescent Kit is a lab equipment product designed for chemiluminescent detection of horseradish peroxidase (HRP)-labeled biomolecules. The kit provides the necessary reagents and components to facilitate sensitive and quantitative analysis of target molecules.

Automatically generated - may contain errors

Lab products found in correlation

Pvdf membrane, by Merck Group (4 mentions) Bca protein assay kit, by Tiangen Biotech (2 mentions) Ripa buffer, by Solarbio (2 mentions) Mrp1 antibody, by Cell Signaling Technology (1 mentions) Stmn1, by Abcam (1 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by Yeasen (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Polyvinylidene difluoride pvdf membrane, by Bio-Rad (1 mentions) Super gelred, by US Everbright (1 mentions) Polyvinylidene fluoride (pvdf), by Abcam (1 mentions) Polyvinylidene fluoride (pvdf), by Bio-Rad (1 mentions) Plant protein extraction kit, by CWBIO (1 mentions) Polyvinylidene fluoride membrane, by Merck Group (1 mentions) Rabbit anti gfp antibody, by Thermo Fisher Scientific (1 mentions) Phospho threonine tyrosine antibody, by Cell Signaling Technology (1 mentions) Goat anti rabbit igg, by OriGene (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Avantor (1 mentions) Np 40 lysis buffer, by Avantor (1 mentions) Protein g agarose beads, by Roche (1 mentions) Goat anti mouse igg, by OriGene (1 mentions)

19 protocols using pro light hrp chemiluminescent kit

1

Western Blot Analysis of c-myc-TaRIM1

Check if the same lab product or an alternative is used in the 5 most similar protocols
The protein expression of introduced c-myc-TaRIM1 was evaluated by Western blotting. Total proteins were extracted from 0.4 g of ground base stem powders. About 10 μg of total soluble proteins were separated on 12% sodium dodecyl sulfate polyacrylamide gels and transferred to PVDF membrane. The Western blots were incubated with a 2000-fold dilution of c-myc antibody (TransGen Biotech, China) at 4 °C for 12 h, and then with 1000-fold dilution of secondary antibody conjugated to horseradish peroxidase (TransGen Biotech, China) at ~25 °C for 1 h. The expressing myc-TaRIM1 proteins were visualized using the Pro-light HRP Chemiluminescent Kit (TIANGEN Biotech, China).
Shan T., Rong W., Xu H., Du L., Liu X, & Zhang Z. (2016). The wheat R2R3-MYB transcription factor TaRIM1 participates in resistance response against the pathogen Rhizoctonia cerealis infection through regulating defense genes. Scientific Reports, 6, 28777.
+ Open protocol
+ Expand
2

Western Blot Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples containing equal amounts of proteins as indicated in the text were resolved by SDS polyacrylamide gel electrophoresis and transferred to PVDF membranes. The membranes were blocked with 5% BSA at room temperature for 1 h and then probed with primary antibodies overnight at 4 °C and incubated with the HRP-conjugated secondary antibodies for 1 h at room temperature. HRP was detected using the Pro-light HRP Chemiluminescent Kit (Tiangen Biotech, Beijing, China) and FluorChem E System (ProteinSimple, Santa Clara, CA, United States).
Wang J., Sun P., Wang Q., Zhang P., Wang Y., Zi C., Wang X, & Sheng J. (2019). (−)-Epigallocatechin-3-gallate derivatives combined with cisplatin exhibit synergistic inhibitory effects on non-small-cell lung cancer cells. Cancer Cell International, 19, 266.
+ Open protocol
+ Expand
3

Western Blot Analysis of EZH2 and MRP1

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein was extracted from T24/CDDP cells and determined the concentration with BCA Protein Assay Kit (TianGen, China) according to manufacturer's instructions. Protein samples (20 mg) were handled with SDS-PAGE gels electrophoresis and transferred to PVDF membranes. The PVDF membrane was first hybridized with EZH2 or Multidrug Resistance Associated Protein 1 (MRP1) antibody (4905 and 72202, Cell Signaling Technology, USA), and then hybridized with secondary antibody. Then, the PVDF membrane was incubated with Pro-light HRP Chemiluminescent Kit (TianGen, China), and photographic images were taken. The intensity of the indicated bands was quantified by Image J (National Institutes of Health, USA) and normalized to the reference standard (GAPDH gene).
Li B., Xie D, & Zhang H. (2019). MicroRNA-101-3p advances cisplatin sensitivity in bladder urothelial carcinoma through targeted silencing EZH2. Journal of Cancer, 10(12), 2628-2634.
+ Open protocol
+ Expand
4

Western Blot Analysis of Protein Expression

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein from HTR-8 cells was prepared using 1× sodium dodecyl sulfate (SDS) lysis buffer (60 mmol/L Tris-HCl [pH 6.8], 10% glycerol, 2% SDS, sodium salt, 0.01% bromophenol blue, 100 mmol/L DL-dithiothreitol). Then, the mixture was heated in a water bath for 8 min at 100°C. Equal amounts of protein samples were subjected to 12% polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride (PVDF) membranes (Bio-Rad Laboratories, Richmond, CA, USA). Then, 5% non-fat milk in Tris-buffered saline with Tween (TBST) was used to block the PVDF membranes for 1 h at room temperature. The membranes were incubated with antibodies against STMN1 (1:2000; Abcam), TTP (1:500; Abcam), p53 (1:1000; Cell Signaling Technology), p-p53 (1:1000; Cell Signaling Technology), p21 (1:1000; Cell Signaling Technology), α-tubulin (1:1000; Yeasen, Shanghai, China), or GAPDH (1:1000; Cell Signaling Technology) overnight at 4°C, followed by horseradish peroxidase (HRP)-conjugated secondary antibody (1:2000; Yeasen) for 1 h. Signals were detected using a Prolight HRP chemiluminescent kit (Tiangen Biotech, Beijing, China) according to the manufacturer’s instructions [27 (link)].
Ma X.L., Li X.C., Tian F.J., Zhang S.M., Liu X.R., Zhang Y., Fan J.X, & Lin Y. (2017). Effect of the p53–tristetraprolin–stathmin-1 pathway on trophoblasts at maternal–fetal interface. PLoS ONE, 12(6), e0179852.
+ Open protocol
+ Expand
5

RANKL-Induced Osteoclastogenesis Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
RAW264.7 cells (4 × 105 cells/well) were seeded in 60-mm plates and incubated overnight, and they were then treated with RANKL (50 ng/mL) in the absence or presence of XLGB (10 μg/mL) or PTE (10, 20, or 40 μg/mL) for 48 h.
Western blot analysis was performed as previously described (Cai et al., 2016 (link)). Briefly, cell lysates were prepared from cultured cells using RIPA buffer (Solarbio, Beijing, China) according to the manufacturer’s protocol. Cell extracts were normalized to determine protein concentration by the BCA method. The proteins were separated by SDS–PAGE and then transferred to PVDF membranes (EMD Millipore Corporation, Merck Life Sciences, KGaA, Darmstadt, Germany). After gentle washing, blocking, and incubation with the primary antibody, the membrane was incubated with an appropriate horseradish peroxidase-conjugated secondary antibody, and the bands were detected using a Pro-light HRP Chemiluminescent Kit (Tiangen Biotech, Beijing, China). The images were acquired with a FluorChem E System (ProteinSimple, Santa Clara, CA, United States).
Liu T., Ding S., Yin D., Cuan X., Xie C., Xu H., Wang X, & Sheng J. (2017). Pu-erh Tea Extract Ameliorates Ovariectomy-Induced Osteoporosis in Rats and Suppresses Osteoclastogenesis In Vitro. Frontiers in Pharmacology, 8, 324.
+ Open protocol
+ Expand
6

Comprehensive Analysis of Chloroplast Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polyclonal antibody against BFA2 was raised in rabbits using the recombinant BFA2 protein (amino acids 62–300 of BFA2). Chlorophyll fluorescence analysis, thylakoid membrane and stromal protein isolation, BN-PAGE, 2D/SDS-PAGE, and immunoblot analysis were performed as previously described (Li et al., 2019 (link)). The gH+ was monitored with the Dual PAM-100 according to previously described methods (Rott et al., 2011 (link); Zhang et al., 2018 (link)). Polysome association analyses were performed as previously described (Zhang et al., 2018 (link)). The rRNAs were stained by Super GelRed (US Everbright Inc., Suzhou, China) and used as fractionation and loading controls. Chloroplast protein labeling and chase was performed as previously described (Zhang et al., 2016 (link), 2018 (link)). Immunoblot signals were detected with a Pro-light HRP Chemiluminescent Kit (TIANGEN) and visualized with a LuminoGraph chemiluminescence analyzer (ATTO). Antibodies against CF1α (PHY0311), CF1β (PHY0312), CF1γ (PHY0313), CF1ε (PHY0314), CF1δ (PHY0315), CFoI (PHY0316), CFoII (PHY0170S), PsaA (PHY0342), PsaD (PHY0343), D1 (PHY0057), D2 (PHY0060), Cyt f (PHY0321), and NdhN (PHY0335) were obtained from PhytoAB (United States).
Zhang L., Zhou W., Che L., Rochaix J.D., Lu C., Li W, & Peng L. (2019). PPR Protein BFA2 Is Essential for the Accumulation of the atpH/F Transcript in Chloroplasts. Frontiers in Plant Science, 10, 446.
+ Open protocol
+ Expand
7

Maleimide-based Cross-linking Assay for Bacterial Chemoreceptors

Check if the same lab product or an alternative is used in the 5 most similar protocols
TMEA (Tris-[2-maleimidoethyl-amide]) is a maleimide-containing cross-linker, which has three functional groups that cross-link sulfhydryls within a distance 10.3 Å. The crosslinking assay was performed as previously described (Studdert & Parkinson, 2007 ). The MCP-null strain CNB-1Δ20 carrying the corresponding cysteine replacement in a plasmid was grown to log-phase at 30 °C. The cells were harvested by low-speed centrifugation and washed twice with KEP buffer (10 mM potassium phosphate, pH 7.0, 0.1 mM EDTA) and subsequently resuspended to OD600=2. After incubation at 30 °C for 5 min, a ligand or a buffer was added to 100 μl cells to a final concentration of 10 mM for another 1 min incubation. TMEA was added to the cell mixture to 50 μM for 10 min, followed by quenching with 10 mM NEM. The samples were boiled in SDS-loading buffer, separated by 8% SDS-PAGE and transferred to a PVDF membrane (Amersham Biosciences Europe GmbH, Freiburg, Germany). MCP2201 was probed with a primary monoclonal antibody targeted to a His tag at a 1:2000 dilution and with a secondary antibody (goat anti-mouse HRP conjugate) at a 1:3000 dilution (Transgen, Beijing, China). Visualization was carried out with a Pro-light HRP Chemiluminescent Kit (Tiangen, Beijing, China).
Hong Y., Huang Z., Guo L., Ni B., Jiang C.Y., Li X.J., Hou Y.J., Yang W.S., Wang D.C., Zhulin I.B., Liu S.J, & Li D.F. (2019). The ligand-binding domain of a chemoreceptor from Comamonas testosteroni has a previously unknown homotrimeric structure. Molecular microbiology, 112(3), 906-917.
+ Open protocol
+ Expand
8

Placental Adiponectin Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein from WT or NOD mouse placenta or BeWo cells was isolated using radioimmunoprecipitation assay buffer (Pierce, Waltham, MA, USA) and then centrifuged at 12,000 × g for 15 min at 4°C. Equal amounts of protein samples were subjected to 10% polyacrylamide gel electrophoresis under denaturing conditions (SDS‐PAGE) and transferred onto polyvinylidene difluoride (PVDF; Bio‐Rad Laboratories, Richmond, CA, USA) membranes. After transfer, the PVDF membranes were blocked with 5% non‐fat milk in TBST for 1 hr at room temperature and then incubated overnight at 4°C with primary antibodies against adiponectin (1:1000 dilution; Abcam, Cambridge, UK) or α‐Tubulin (1:500 dilution; Boster, Wuhan, China). At room temperature, the membranes were washed three times with TBST for 5 min each time and then probed with a secondary antibody for 1 hr. Signals were detected using the Pro‐light HRP Chemiluminescent Kit (Tiangen Biotech, Beijing, China) according to the manufacturer's instructions. Gel Pro software was used to obtain quantitative data from Western blots. Detection of α‐Tubulin was used as a loading control.
Qin C., Tian F., Liu X., Wu F., Ma X, & Lin Y. (2016). CpG Oligodeoxynucleotides Downregulate Placental Adiponectin and Increase Embryo Loss in Non‐Obese Diabetic Mice. American Journal of Reproductive Immunology, 76(1), 38-49.
+ Open protocol
+ Expand
9

Immunodetection of Recombinant Wheat Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total proteins of leaf samples (0.1 g each) from the transgenic and WT wheat lines were extracted using a plant protein extraction kit (CWBIO, China). Total proteins were separated on a 12% SDS‐PAGE gel and then transferred to a PVDF membrane (Millipore, USA). The TaSTT3b‐2B‐His protein was incubated with 2000‐fold diluted anti‐His antibody followed by a secondary antibody conjugated to horseradish peroxidase (HRP). After incubating overnight at 4 °C, the TaSTT3b‐2B‐His protein was visualized using the Pro‐light HRP Chemiluminescent Kit (TIANGEN, China).
Zhu X., Rong W., Wang K., Guo W., Zhou M., Wu J., Ye X., Wei X, & Zhang Z. (2021). Overexpression of TaSTT3b‐2B improves resistance to sharp eyespot and increases grain weight in wheat. Plant Biotechnology Journal, 20(4), 777-793.
+ Open protocol
+ Expand
10

Western Blot Analysis of GFP Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK 293T cells were lysed in lysis buffer (140 mM NaCl, 5 mM EDTA, 10 Mm Tris–HCl, and 0.2% Triton X-100) supplemented with 1 × protease inhibitor cocktail. After centrifugation, the supernatant was electrophoresed on 9% sodium dodecyl sulfate polyacrylamide gel and transferred to polyvinylidene fluoride membranes (Millipore). Membranes were blocked with 5% skimmed milk in TBST buffer (50 mM Tris–HCl, 140 mM NaCl, pH 7.4, with 0.05% Tween-20) at room temperature for 1 h, followed by incubation with rabbit anti-GFP antibody (1:2,000, Invitrogen, A11122) and horseradish peroxidase (HRP)-conjugated anti-GAPDH antibody (1:8,000, Kangchen, KC-5G4) at 4°C overnight. The membranes were rinsed three times the next day before incubating with HRP-conjugated anti-rabbit secondary antibody for 3 h at room temperature. Bands were visualized by Pro-Light HRP Chemiluminescent Kit (TIANGEN, PA112).
Liu J.W., Li H, & Zhang Y. (2022). Npas3 regulates stemness maintenance of radial glial cells and neuronal migration in the developing mouse cerebral cortex. Frontiers in Cellular Neuroscience, 16, 865681.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!