Chromatographic pure methanol

Glycholithocholic acid (GLCA) standards were purchased from Toronto Research Chemicals; Cholic acid (CA), lithocholic acid (LCA), deoxycholic acid (DCA), ursodeoxycholic acid (UDCA), chenodeoxycholic acid (CDCA), taurocholic acid (TCA), glychocholic acid (GCA), taurolithocholic acid (TLCA), taurodeoxycholic acid (TDCA), tauroursodeoxycholic acid (TUDCA), taurochenodeoxycholic acid (TCDCA), glychodeoxycholic acid (GDCA), glychochenodeoxycholic acid (GCDCA), glychoursodeoxycholic acid (GUDCA) standards, and isotope-labeled internal standard deuterocholic acid (d4-CA), deuterodeoxycholic acid (d4-DCA), deuteroglychocholic acid (d4-GCA), deuterated ursodeoxycholic acid (d4-UDCA), and deuterated lithocholic acid (d4-LCA) were purchased from Sigma Aldrich; Chromatographic pure acetonitrile and chromatographic pure methanol were purchased from Merck, Germany; Chromatographic grade formic acid was purchased from Shanghai Aladdin.

A total of 14 phenolic acids and flavonoids standards with a purity more than 98% were purchased from Sigma. These included (+)-catechin, epicatechin, gallic acid, ferulic acid, chlorogenic acid, caffeic acid, rutin, spinosin, quercetin, phloridzin, isorhamnetin, luteolin, kaempferol, and jujubosideA. In addition, chromatographic pure methanol, formic acid, and acetonitrile were purchased from Merck.

Zheshen No. 2 P. Heterophylla (Miq.) Pax Chinese herbal was purchased from Ningde Nanling Agricultural Co., Ltd. Zherong County of the Ningde region of the Fujian Province in China is the advocate producing area of TZS. The TZS was cut into pieces and then dried below 60°C.
Chromatographic pure methanol was purchased from Merck KGaA (Darmstadt, Germany). Chromatographic pure acetonitrile was purchased from Fisher Scientific UK Ltd. (Leicestershire, England). Analytical grade reagents including petroleum ether, ethyl acetate, ethanol, n-butanol, ammonia, and chloroform were bought from Xilong Scientific Co., Ltd. (Shantou, China). Interleukin-10 (IL-10) and tumor necrosis factor (TNF-α) enzyme linked immunosorbent assay (ELISA) kits were purchased from cloud-clone corp.