Nickel-nitrilotriacetic acid (Ni-NTA) Sepharose was procured from Solarbio (Beijing, China). OTA, OTC, and fumonisin B1 (FB1) were obtained from Pribolab (Singapore). Aflatoxin B1 (AFB1) and zearalenone (ZEN) were purchased from Fermentek (Jerusalem, Israel). OTB was from Bioaustralis (Smithfield, NSW, Australia). Deoxynivalenol (DON) was from Sigma-Aldrich (CA, USA). Bovine serum albumin (BSA) and ovalbumin (OVA) were obtained from Sangon Biotech Inc. (Shanghai, China). The black microtiter plates were from Corning Inc. (NY, USA). The mouse anti-OTA monoclonal antibody mAb YK232 was obtained from Yikang Biotech Inc. (Haikou, China). The anti-alpha fetoprotein (AFP) Nb was prepared in our lab. The engineered BL21(DE3) strain of E. coli containing the vector Nb28-pET25b for Nb28 expression was prepared previously. All other chemicals and organic solvents were of reagent grade or better.
Fluorescence spectra and UV–vis spectra were obtained using a spectral scanning multimode reader SP-Max 3500FL (Flash Spectrum Inc., Shanghai, China). The HPLC system consisted of a Model E2695 pump, a Model 2475 fluorescence detector, and a SunFire C18 5 μm column (Waters, MA, USA).
Fluorescence spectra and UV–vis spectra were obtained using a spectral scanning multimode reader SP-Max 3500FL (Flash Spectrum Inc., Shanghai, China). The HPLC system consisted of a Model E2695 pump, a Model 2475 fluorescence detector, and a SunFire C18 5 μm column (Waters, MA, USA).