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Aura imaging software

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Aura imaging software is a core product of Spectral Instruments Imaging. It is a software application designed to analyze and process imaging data collected from various scientific instruments.

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Lab products found in correlation

Lago x, by Spectral Instruments Imaging (4 mentions) D luciferin, by GoldBio (3 mentions) Ivis spectrum, by PerkinElmer (2 mentions) D luciferin luck 2g, by GoldBio (2 mentions) Living image software, by PerkinElmer (2 mentions) Ami htx, by Spectral Instruments Imaging (1 mentions) C57bl 6 mice, by Jackson ImmunoResearch (1 mentions) Amix optical imaging system, by Spectral Instruments Imaging (1 mentions) Balb c, by Jackson ImmunoResearch (1 mentions) Matrigel, by Corning (1 mentions) D luciferin, by Spectral Instruments Imaging (1 mentions) Pmdlg prre, by Addgene (1 mentions) Pmd2 g, by Addgene (1 mentions) Prsv rev, by Addgene (1 mentions) Effectene transfection reagent, by Qiagen (1 mentions) Stereomicroscope, by Leica (1 mentions) D luciferin, by PerkinElmer (1 mentions) Nod scid il2rgammanull nsg mice, by Jackson ImmunoResearch (1 mentions) Ivis 200 system, by PerkinElmer (1 mentions) Vivoglo luciferin, by Promega (1 mentions)

25 protocols using aura imaging software

1

In vivo Renal Targeting of Peptide-Conjugated Micelles

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To test the renal targeting ability in vivo, 100 μl of 1,000 μM micelles containing 90:10 molar ratio of DSPE‐PEG‐peptide:DSPE‐PEG‐Cy7 or 45:45:10 molar ratio of DSPE‐PEG‐methoxy:DSPE‐PEG‐peptide:DSPE‐PEG‐Cy7 or PBS were tail vein injected with in 6–7 week old male and female C57BL/6 mice (n = 4, Jackson Laboratories, Bar Harbor, ME). After 24 hours in circulation, mice were euthanized and their organs (i.e., brain, heart, lungs, liver, spleen, intestines, kidneys, and bladder) were harvested and imaged ex vivo via Ami HTX (Spectral Instruments Imaging, Tucson, AZ). Qualification of the fluorescence signal was conducted to determine the biodistribution of particles by Aura imaging software (Spectral Instruments Imaging, Tucson, AZ). All animal experiments were approved by University of Southern California (USC) Institutional Animal Care and Use Committee (IACUC).
Huang Y., Jiang K., Zhang X, & Chung E.J. (2020). The effect of size, charge, and peptide ligand length on kidney targeting by small, organic nanoparticles. Bioengineering & Translational Medicine, 5(3), e10173.
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2

Orthotopic Breast Cancer Model in Mice

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4Tl cells expressing the luciferase gene were orthotopically implanted
in syngeneic Balb/c (Jackson Laboratory, Main USA). All the mice were between
5–6 weeks of age and weighing 18–20 g. Animals were anesthetized
using a mixture of Xylazine (20 mg kg−1) and Ketamine (100 mg
kg−1) administered intraperitoneally. Hair was removed for
the right half of the abdomen by using hair removal ointment, and then abdomen
was cleaned by povidone-iodine and alcohol. A small incision was made in the
middle of the abdomen, and the skin was separated from the peritoneum using
blunt forceps. Separated skin was pulled to the right side to expose the mammary
fat pad and 50 000 4Tl cells in 50 μL Matrigel (Corning, NY, USA) were
injected. Tumor growth was monitored every week. In vivo, optical images were
obtained every week to keep track of primary tumor and metastasis development by
injecting 100 μL of luciferin (dose 150 mg kg−1)
intraperitoneally followed by the acquisition of bioluminescence signal by
spectral AmiX optical imaging system (Spectral instruments imaging, Inc.,
Tucson, AZ). The photon intensity per mm per s was determined by Aura imaging
software by Spectral Instruments Imaging, LLC (version 2.2.1.1). The animals
were anesthetized using an isoflurane vaporizer chamber (2.5% Iso: 2 ± 3
L min−1 O2) and maintained under anesthesia (2%
with oxygen) during the procedure.
Rashid M.H., Borin T.F., Ara R., Alptekin A., Liu Y, & Arbab A.S. (2020). Generation of Novel Diagnostic and Therapeutic Exosomes to Detect and Deplete Protumorigenic M2 Macrophages. Advanced therapeutics, 3(7), 1900209.
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3

Bioluminescent Imaging of Mice

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NSGS mice were injected intraperitoneally with 150mg/kg D-luciferin (XenoLight). Ten minutes later, mice were anesthetized and imaged with Lago X (Spectral Instruments Imaging). Bioluminescent signals were quantified using Aura imaging software (Spectral Instruments Imaging). Total flux values were determined and are presented as photons (p)/second (sec).
Huang F., Sun J., Chen W., He X., Zhu Y., Dong H., Wang H., Li Z., Zhang L., Khaled S., Marcucci G., Huang J, & Li L. (2020). HDAC4 inhibition disrupts TET2 function in high-risk MDS and AML. Aging (Albany NY), 12(17), 16759-16774.
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4

In Vivo Fluorescence Imaging of Atsttrin

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Atsttrin and E5C were labeled with Vivo-Tag 680-S in accordance with the manufacturer’s protocol excepting that conjugation was carried out overnight at 4°C. Free dye was removed using a 5-minute centrifugation at 4000 rpm in a 3kDa MWCO Amicon filter. Adult DBA1/J mice, approximately 4 months of age, were anesthetized and hind limbs were shaved. 10 μL of PBS, Vivo-Tag 680-S labeled-E5C gel, Vivo-Tag 680-S-labeled Atsttrin in E5C gel, or Vivo-Tag 680-S-labeled Atsttrin in PBS were intra-articularly injected. Two mice were implemented in each group and injections were performed bilaterally to generate 4 regions of interest. Mice were imaged using Living Image® software on a PerkinElmer IVIS Spectrum imaging system (PerkinElmer, MA, USA) at days 0, 28 and 32 days (excitation 675 nm/ emission 725 nm). The fluorescence signal (p/s/cm2/sr) was normalized to background and measured using Aura imaging software (Spectral Instruments Imaging).
Katyal P., Hettinghouse A., Meleties M., Hasan S., Chen C., Cui M., Sun G., Menon R., Lin B., Regatte R., Montclare J.K, & Liu C.J. (2022). Injectable Recombinant Block Polymer Gel for Sustained Delivery of Therapeutic Protein in Post Traumatic Osteoarthritis. Biomaterials, 281, 121370.
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5

Bone Marrow Homing and Leukemia Survival

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For bone marrow homing experiments, 107 cells were injected via the tail vein into NSG mice (n = 3–4 per group). Sixteen hours later, bone marrows were analyzed by FACS for CD19, CD10, and eGFP-positive cells. Results are expressed as cell percentage in the live cell lymphocyte gate. To measure survival, non-irradiated NSG mice 8–10 weeks of age were used in all experiments. Female [n = 5 for US7/EV and n = 7 US7/OE] or male mice [n = 5 per group] were injected with 2 × 106 leukemia cells on d0. Imaging for leukemia signal was performed once per week by i.p. injection of 2.5 mg of D-luciferin in 200 μl of PBS. End points included loss of >20% initial body weight. For vincristine treatment, we used n = 5 female mice per group. Mice received six weekly vincristine treatments [0.5 mg/kg; i.p.] starting on d14. Bioluminescence signals were quantified using Aura imaging software (Spectral Instruments Imaging, LLC Tucson, AZ).
All animal experiments were conducted under an IACUC-approved institutional protocol. Methods of euthanasia were consistent with the guidelines of the American Veterinary Medical Association.
Zhang M., Qi T., Yang L., Kolarich D, & Heisterkamp N. (2022). Multi-Faceted Effects of ST6Gal1 Expression on Precursor B-Lineage Acute Lymphoblastic Leukemia. Frontiers in Oncology, 12, 828041.
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6

Lentiviral Transduction and In Vivo Bioluminescence Imaging

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Prior to in vivo bioluminescence imaging, 3rd generation luciferase expression lentivirus was generated by co-transfection of 1.5 μg pLenti CMV Puro LUC plasmid (17477, Addgene) (Campeau et al., 2009 (link)), 0.5 μg pMD2.G (12259, Addgene), 0.3 μg pMDLg/PRRE (12251, Addgene), 0.7 μg pRSV-Rev (12253, Addgene) into HEK293T cells in 60 mm cell culture dish with Effectene Transfection Reagent (301427, QIAGEN), and then the leukemia cells or the tumor cells were infected with lentivirus and selected with 1 μg/ml puromycin to stably express luciferase. For in vivo bioluminescence imaging, the mice were weighed, injected intraperitoneally with 150 mg/kg D-luciferin (LUCK-2G, Goldbio) in PBS solution, and then anesthetized with isoflurane. The mice were imaged 10 minutes after D-luciferin injection with Lago X (Spectral Instruments Imaging). The bioluminescent signals were quantified using Aura imaging software (Spectral Instruments Imaging). Total flux values were determined by drawing regions of interest, which are identical among the mice in different group, and are presented in photons/second/cm2/steradian.
Su R., Dong L., Li Y., Gao M., Han L., Wunderlich M., Deng X., Li H., Huang Y., Gao L., Li C., Zhao Z., Robinson S., Tan B., Qing Y., Qin X., Prince E., Xie J., Qin H., Li W., Shen C., Sun J., Kulkarni P., Weng H., Huang H., Chen Z., Zhang B., Wu X., Olsen M.J., Müschen M., Marcucci G., Salgia R., Li L., Fathi A.T., Li Z., Mulloy J.C., Wei M., Horne D, & Chen J. (2020). Targeting FTO suppresses cancer stem cell maintenance and immune evasion. Cancer cell, 38(1), 79-96.e11.
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7

Quantifying Metastatic Colonization in Mice

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shControl or PLEC-KD DU145 (1×105 each) cells transduced with luciferase and RFP were injected intracardially into left ventricles of NSG mice to evaluate in vivo metastatic colonization. Whole body bioluminescence was measured at Day 0 and at end point, Day 29. Mice were injected with 5 mg/kg D-Luciferin (Perkin Elmer), and after 5 minutes were imaged on a LAGO imager using Aura imaging software (Spectral Instruments Imaging) at Stanford Preclinical Imaging Facility at Porter Drive. After Day 29 imaging, mice were sacrificed, and the lungs, liver, hind limbs, genitourinary tract (GU), and kidneys were harvested and fixed in 10% formalin overnight. Intact tissues were imaged on Leica stereomicroscope under brightfield and RFP filters for metastatic site identification. After imaging, tissues were processed as previously described for histological analysis.
Buckup M., Rice M.A., Hsu E.C., Garcia-Marques F., Liu S., Aslan M., Bermudez A., Huang J., Pitteri S.J, & Stoyanova T. (2020). Plectin is a Regulator of Prostate Cancer Growth and Metastasis. Oncogene, 40(3), 663-676.
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8

In Vivo Evaluation of CAR T Cells in DLBCL Xenograft

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NOD-scid IL2Rgammanull (NSG) mice (Jackson Laboratories, 005557) were housed at the Boston Children's Hospital animal care facility following institutional guidelines. 8 to 12-week-old male and female mice were intravenously injected with 1x106 OCI-Ly1 DLBCL tumor cells expressing green firefly luciferase. The inoculated animals were subjected to bioluminescence imaging (BLI) using the IVIS 200 system (PerkinElmer) twice per week following intraperitoneal injections of Vivoglo luciferin (Promega, P1043) at 150mg/kg body weight. After two weeks, animals with substantial tumor cell engraftment (Mean total flux > 5x105 photons/sec) were randomly assigned into four groups and intravenously injected with PBS (untreated) or 2x106CAR T cells generated from control iPSC-SF-T, iPSC-EZ-T, or PBMC-T cells. Tumor burden was measured by BLI weekly, and images were processed and analyzed using Aura imaging software (Spectral Instruments Imaging). To monitor CAR T cell persistence, peripheral blood cells were collected via retro-orbital bleeding after 3 weeks of T cell injections, and absolute numbers of CAR T cells were determined by flow cytometry analysis. All animal experiments were performed under protocols approved by the Institutional Animal Care and Use Committee of Boston Children's Hospital.
Jing R., Scarfo I., Najia M., da Rocha E.L., Han A., Sanborn M., Bingham T., Kubaczka C., Jha D., Falchetti M., Schlaeger T.M., North T.E., Maus M.V, & Daley G.Q. (2022). EZH1 repression generates mature iPSC-derived CAR T cells with enhanced antitumor activity. Cell stem cell, 29(8), 1181-1196.e6.
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9

In Vivo Imaging of Cold-Induced Glucose Uptake

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2-NBDG (3 mM in PBS, 0.3 mL; APEXBIO, cat#B6035) or PBS (negative control) was administered to mice at 10–12 weeks of age via a tail-vein injection after 2 h cold treatment with fasting, and mice were exposed in cold for another 1 h before imaging69 (link),70 (link). Images were taken with the Ami optical imaging system (Spectral Instruments Imaging). After 3 h cold treatment, mice were immediately euthanized and dissected. Multiple tissues, including the BAT, iWAT, tibialis anterior (TA) muscle, quadriceps (QU) muscle, gastrocnemius (GAS) muscle, liver, heart, spleen, and kidney, were collected, followed by the ex vivo imaging (ex/em: 465/530 nm). The fluorescent images were analyzed by AURA Imaging Software (Spectral Instruments Imaging).
Qiu J., Yue F., Zhu P., Chen J., Xu F., Zhang L., Kim K.H., Snyder M.M., Luo N., Xu H.W., Huang F., Tao W.A, & Kuang S. (2023). FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes. Nature Communications, 14, 6344.
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10

Lentiviral Knockdown in Xenograft Mouse Model

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MONO-MAC-6 cells were lentivirally infected with pLenti CMV Puro LUC (17477, Addgene) by two rounds of ‘spinoculation’ and selected with puromycin for 7 days. Then, the luciferase-labeled MONO-MAC-6 cells were infected with lentivirus to knock down FTO, PFKP, or LDHB prior to xenograft transplantation. For in vivo bioluminescence imaging, NRGS mice were weighted, injected intraperitoneally with D-luciferin (LUCK-2G, Goldbio) at 150 mg/kg, and anesthetized with isoflurane. The mice were imaged using a Lago X (Spectral Instruments Imaging) 10 minutes post D-luciferin injection and Aura imaging software (Spectral Instruments Imaging) was used to quantify the bioluminescent signals. Radiance is in the unit of “photons/second/cm2/steradian”.
Qing Y., Dong L., Gao L., Li C., Li Y., Han L., Prince E., Tan B., Deng X., Wetzel C., Shen C., Gao M., Chen Z., Li W., Zhang B., Braas D., ten Hoeve J., Sanchez G.J., Chen H., Chan L.N., Chen C.W., Ann D., Jiang L., Müschen M., Marcucci G., Plas D.R., Li Z., Su R, & Chen J. (2021). R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis. Molecular cell, 81(5), 922-939.e9.
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