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Bioapex ftms

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The Bioapex-FTMS is a high-performance Fourier Transform Mass Spectrometer (FTMS) designed for advanced biological and biomolecular applications. It utilizes state-of-the-art technology to provide accurate mass measurements and in-depth analytical capabilities.

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Lab products found in correlation

Mercury 400 mhz spectrometer, by Bruker (2 mentions) Mercury 400 mhz spectrometer, by Agilent Technologies (2 mentions) Avance drx 500, by Agilent Technologies (2 mentions) Clorgyline, by Merck Group (1 mentions) Kynuramine dihydrobromide, by Merck Group (1 mentions) Phenelzine sulfate, by Merck Group (1 mentions) R deprenyl hydrochloride, by Merck Group (1 mentions) 4 hydroxyquinoline, by Merck Group (1 mentions) K2hpo4 buffer, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Silica gel 60 f254, by Merck Group (1 mentions) Avance drx spectrometer, by Bruker (1 mentions) Bbfo smart probe, by Bruker (1 mentions) 400 mhz aeon nitrogen free magnet, by Bruker (1 mentions) Avance 3 400 mhz, by Bruker (1 mentions) Autopol 4 automatic, by Rudolph Research Analytical (1 mentions) 400 mhz nmr spectrometer, by Bruker (1 mentions) 500 mhz nmr spectrometer, by Bruker (1 mentions) J 715 spectrometer, by Jasco (1 mentions) Delta prep 4000, by Waters Corporation (1 mentions)

9 protocols using bioapex ftms

1

Spectroscopic Analysis and Monoamine Oxidase Inhibition

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The 1H, 13C, and 2D NMR spectra were recorded on a Varian Mercury 400 MHz spectrometer, Bruker Avance DRX spectrometer at 600 MHz (1H) and 150 MHz (13C) using TMS as an internal standard. The HR-ESI-MS were done using a Bruker Bioapex-FTMS with electrospray ionization. Adsorbents for column Chromatography including Diaion HP-20, Silica gel 60 F254 (0.2 mm, Merck), MN-polyamide-SC-6, and Sephadex™ LH-20. Human recombinant MAO-A and -B were obtained from BD Biosciences. Clorgyline, R-(-)-deprenyl hydrochloride, Kynuramine dihydrobromide, phenelzine sulfate, 4-hydroxyquinoline, K2HPO4 buffer, and DMSO were purchased from Sigma.
Mohamed S.M., Chaurasiya N.D., Mohamed N.M., Bayoumi S.A., Tekwani B.L, & Ross S.A. (2020). Promising selective MAO-B inhibition by sesamin, a lignan from Zanthoxylum flavum stems. Saudi Pharmaceutical Journal : SPJ, 28(4), 409-413.
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2

Comprehensive NMR and MS Analysis

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1D and 2D NMR spectra were recorded on a Bruker Avance III 400 MHz with BBFO Smart Probe and Bruker 400 MHz AEON Nitrogen-Free Magnet (Bruker AG, Switzerland) using the chemical shift of CDCl3 solvent peak at 7.24 (s) ppm in 1H and 77.2 (t) ppm in 13C NMR as an internal reference standard. Data were analyzed using Topspin 3.1 Software. LC-ESIMS was obtained using a Bruker Bio Apex FT-MS in ESI mode.
Amin E., Moawad A, & Hassan H. (2016). Biologically-guided isolation of leishmanicidal secondary metabolites from Euphorbia peplus L.. Saudi Pharmaceutical Journal : SPJ, 25(2), 236-240.
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3

Comprehensive Analytical Protocols for Natural Products

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Optical rotations were recorded at ambient temperature using a Rudolph Research Analytical Autopol IV automatic polarimeter. UV spectra were obtained in MeOH using a Hewlett-Packard 8453 UV/vis spectrometer, and ECD data were collected using an Olis Cary-17 spectrophotometer (1 cm path length cell). NMR spectra were acquired on a Bruker 400 MHz NMR spectrometer at 400 (1H) and 100 MHz (13C) and a Bruker 500 MHz NMR spectrometer at 500 (1H) and 125 MHz (13C), using the residual solvent as an internal standard. Multiplicity determinations (DEPT) and 2D-NMR spectra (HMQC, HMBC, NOESY) were obtained using standard Bruker pulse programs. HRESIMS data were acquired by direct injection using a Bruker Bioapex-FTMS with electrospray ionization (ESI). TLC was caonducted on precoated silica gel 60F254, 0.25 mm and reversed phase (RP) C18 F254 silica gel (EMD Chemicals Inc., Darmstadt, Germany). Column chromatography was carried out on silica gel G60 (60–120 mesh, Merck, Darmstadt, Germany) and sephadex LH-20 (Mitsubishi Kagaku, Tokyo, Japan). Solid phase extraction (SPE) cartridges C18 (Supelco Inc., Bellefonte, PA, USA) were used in the fractionation experiments. The compounds were visualized by spraying the TLC plates with 1% vanillin–H2SO4 spray reagent.
Mohamed E.I., Zaki M.A., Chaurasiya N.D., Owis A.I., AbouZid S., Wang Y.H., Avula B., Seida A.A., Tekwani B.L, & Ross S.A. (2017). Monoamine oxidases inhibitors from Colvillea racemosa: Isolation, biological evaluation, and computational study. Fitoterapia, 124, 217-223.
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4

Structural Characterization of Compound 2

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The optical rotation of 2 was determined with an Autopol IV instrument at room temperature. IR spectra were obtained using a Bruker Tensor 27 instrument. CD spectrum was measured on a JASCO J-715 spectrometer. NMR spectra were recorded on a Bruker Avance DRX-500 instrument at 500 (1H) and 125 MHz (13C), and a Varian Mercury 400 MHz spectrometer at 400 (1H) and 100 MHz (13C). The HRESIMS spectra were measured using a Bruker Bioapex-FTMS with electrospray ionization (ESI). Column chromatographic separation was performed on silica gel 60 (0.04–0.063 mm) and Sephadex LH-20 (0.25–0.1 mm, Merck). TLC was performed on precoated TLC plates with silica gel 60 F254 (0.2 mm, Merck). Semipreparative HPLC (Waters Delta Prep 4000) was performed using Luna® RP-18 (250 mm, 10 mm, 5 µm). The solvent systems used for TLC analyses were: EtOAc:n-hexane (7:3), CHCl3:MeOH (9.5:0.5), and CHCl3:MeOH (8:2).
Ghoneim M.M., Elokely K.M., El-Hela A.A., Mohammad A.E., Jacob M., Cutler S.J., Doerksen R.J, & Ross S.A. (2014). Isolation and characterization of new secondary metabolites from Asphodelus microcarpus. Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents, 23(7), 3510-3515.
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5

Characterization of Natural Compounds

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The 1H, APT, and 2D NMR experiments were run at 400 and 100 MHz using Bruker Avance III spectrometer, Faculty of Science, Zagazig University. The HR-ESI-MS analysis was performed using a Bruker Bioapex-FTMS with electrospray ionization (USA). Chromatographic adsorbents include silica gel G60 (60–120 mesh, Merck, Darmstadt, Germany), C18-RP silica gel (230–400 mesh, Merck, Darmstadt, Germany), MN-polyamide-SC-6 (50–160 μm, Sorbent Technologies, Norcross, GA, USA), Sephadex® LH-20 (Mitsubishi Kagaku, Tokyo, Japan), Florosil (60–100 mesh, Carlo Erba Reagents, France) and Diaion® HP-20 (Sorbent Technologies, Norcross, GA, USA). TLC was conducted on pre-coated silica 60 F254 aluminum sheets, 0.25 mm and RP-18 F254, 0.25 mm (E-Merck), Darmstadt, Germany). Solvents used for extraction and isolation were of analytical grade, purchased from (Adwic - El Nasr Pharmaceutical Co., Cairo, Egypt). Deuterated solvents for NMR spectral analysis: DMSO-d6, CD3OD, CDCl3, C5D5N (Cambridge Isotope Laboratories, Inc., MA).
Abdel-Moez G., Sayed H., Khalifa A., Abd-Elrahman S., Osman M, & Mohamed S. (2024). Evaluating anthelmintic, anti-platelet, and anti-coagulant activities, and identifying the bioactive phytochemicals of Amaranthus blitum L.. BMC Complementary Medicine and Therapies, 24, 183.
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6

Detailed Analytical Instrumentation Protocol

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Optical rotations, Autopol IV polarimeter; IR, Bruker Tensor 27 instrument; UV, Cary-50 Bio spectrophotometer; CD, JASCO J-715 spectrometer; NMR, Bruker Avance DRX-500 instrument at 500 (1H) and 125 MHz (13C) and a Varian Mercury 400 MHz spectrometer at 400 (1H) and 100 (13C); HR-ESI-MS, Bruker BioApex-FTMS with electrospray ionization (ESI). Column chromatography (CC) was performed on silica gel 60 F254 (0.2 mm, Merck), Diaion HP-20, Sephadex™ LH-20, SPE columns 10 g RP (C-18) and MN-polyamide-SC-6.
Mohamed S.M., Elokely K.M., Bachkeet E.Y., Bayoumi S.A., Carnevale V., Klein M.L., Cutler S.J, & Ross S.A. (2015). New Glycosides and Trypanocidal Metabolites from Vangueria edulis. Natural product communications, 10(11), 1897-1900.
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7

Characterization of Synthetic Compounds

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The 1H and 13C NMR spectra were recorded in DMSO-d6 and CDCl3 on a JEOL-NMR Eclipse-400 MHz spectrophotometer operating at 400 MHz for 1H and 100 MHz for 13C NMR. Chemical shift (δ) values are presented in ppm and in reference to the residual solvent signals of DMSO-d6 and CDCl3 at δH/δC 2.50/39.5 and 7.25/70.2, respectively. The coupling constants value (J) reported in Hz. Genesis II FT-IR spectrometer was also used for all IR spectra. Melting points was measured using a Thermo Scientific Electrothermal Digital Melting Point Apparatus IA9100 series. UV/Vis. absorbance measurements were recorded on Agilent Technologies Cary 8454 UV/Vis. spectrophotometer with a PCB-1500 water Peltier circulating system. Optical rotations were measured on Roudolph Research Analitical AUTOPOL® III polarimeter. HRESIMS data were acquired using a Bruker BioApex-FTMS with electrospray ionization (ESI).
Other common chromatographic techniques such as thin layer chromatography (TLC) on precoated silica gel G254 aluminium plates and silica gel flash column chromatography were also engaged in the purification of the synthesized compounds.
Mingle D., Ospanov M., Radwan M.O., Ashpole N., Otsuka M., Ross S.A., Walker L., Shilabin A.G, & Ibrahim M.A. (2020). First In Class (S,E)-11-[2-(Arylmethylene)Hydrazono]-PBD Analogs As Selective CB2 Modulators Targeting Neurodegenerative Disorders. Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents, 30(1), 98-108.
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8

NMR and MS Analysis of Compounds

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NMR spectra were acquired on a Varian Mercury 400 MHz spectrometer at 400 (1H) and 100 (13C) MHz in CDCl3, using the residual solvent as an internal standard. Multiplicity determinations (DEPT) and 2D-NMR spectra (HMQC, HMBC, NOESY) were obtained using standard Bruker pulse programs. HRESIMS were obtained by direct injection using a Bruker Bioapex-FTMS and Bruker micrOTOF (Bruker Daltonics, Bruker Inc., Billerica, MA, USA) with electrospray ionization (ESI). UHPLC/APCI-MS was performed using an Agilent 1290 UHPLC system coupled with an Agilent 6120 single quadrupole mass spectrometer. TLC was conducted on pre-coated silica gel 60 F254 (EMD Chemicals Inc., Darmstadt, Germany). Centrifugal preparative TLC (CPTLC, using a Chromatotron®, Harrison Research Inc., Palo Alto, CA, USA; model 8924, tagged with a fraction collector) was carried out on a 6 mm silica gel P254 (Analtech, Newark, DE, USA) disk. Samples were dried using a Savant Speed Vac Plus SC210A concentrator. The compounds were visualized by spraying the TLC plates, with 1% vanillin-H2SO4 as a spray reagent. The reference compounds 1–3 were purchased from and the reference standard doxorubicin (purity ≥ 98.0% pure) was procured from Sigma-Aldrich.
Balachandran P., Ibrahim M.A., Zhang J., Wang M., Pasco D.S, & Muhammad I. (2021). Crosstalk of Cancer Signaling Pathways by Cyclic Hexapeptides and Anthraquinones from Rubia cordifolia. Molecules, 26(3), 735.
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9

Analytical Techniques for Structural Elucidation

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ATI Mattson genesis series Fourier transform (FT-IR) infrared spectrophotometer (Midland, Canada) was used to record IR spectra. Hewlett Packard 8452A diode array spectrophotometer (Palo Alto, CA, USA) was used to obtain UV spectra. Bruker Avance DRX 500 MHz and 125 MHz spectrometers (Billerica, MA, USA) were used to obtain 1D and 2D NMR spectra for 1 H and 13 C, respectively. Bruker Bioapex FT-MS was used to obtain the EI mass spectra.
Tyagi R., Alam P., Rehman M.T., Alajmi M.F., Hussain A., Amin S., Mujeeb M., & Mir S.R. (2020). A New Cytotoxic Dimeric Sesquiterpene Isolated from Inula racemosa Hook. f. (Root): In Vitro and In Silico Analyses. Separations, 8(1), 2-2.
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