The floral corn (Zea mays L. saccharata) pollen was gathered from maize farms in Gorgan (Golestan, Iran) in June 2020. Pollens were dehydrated at a temperature of below 40°C, then stored until used. Chemicals including ABTS (2,2′‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulfonic acid)), Trolox (6‐hydroxy‐2,5,7,8‐tetramethychroman‐2‐carboxylic acid), DPPH (1,1 Diphenyl‐2‐picrylhydrazyl), TBA (thiobarbituric acid), ferrozine (3‐(2‐pyridyl)‐5‐6‐diphenyl‐1,2,4‐triazine‐4′,4″ disulphonic acid sodium salt), TCA (Trichloroacetic acid), pyrocatechol violet, and Coomassie brilliant blue (G250) were purchased from Sigma Aldrich Co (St. Louis, Missouri, USA). Hydrogen peroxide (H2O2), potassium persulfate (K2S2O8), ferrous sulfate (FeSo4), ferric chloride (FeCl3), ferrous chloride (FeCl2) copper sulfate (CuSo4), pyridine, dimethyl sulfoxide (DMSO), EDTA (ethylenediaminetetraacetic acid), and potassium ferricyanide provided by Merk (Darmstadt, Germany). Pepsin, trypsin, and pancreatin were obtained from Novo Nordisk A/S (Bagsværd, Denmark).
Pyrocatechol violet
Manufactured by Merck Group
Sourced in United States, Germany
Pyrocatechol violet is a chemical reagent used in analytical chemistry. It is a coloring agent that undergoes a color change in the presence of certain metal ions, making it useful for detection and analysis purposes.
Automatically generated - may contain errors
Lab products found in correlation
Ascorbic acid, by Merck Group (7 mentions)
Ferrozine, by Merck Group (6 mentions)
Ammonium molybdate, by Merck Group (6 mentions)
Gallic acid, by Merck Group (6 mentions)
Sulfuric acid, by Merck Group (5 mentions)
Methionine, by Merck Group (4 mentions)
Sodium bicarbonate, by Thermo Fisher Scientific (4 mentions)
Ferrous sulfate 2, by Merck Group (4 mentions)
Potassium ferricyanide, by Merck Group (4 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (4 mentions)
Bovine serum albumin (bsa), by Merck Group (4 mentions)
Riboflavin, by Merck Group (3 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Cultilab (3 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (3 mentions)
Trichloroacetic acid, by Merck Group (3 mentions)
Quercetin, by Merck Group (3 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (3 mentions)
Phosphoric acid, by Merck Group (3 mentions)
25 protocols using pyrocatechol violet
1
Antioxidant Potential of Maize Pollen
2
Antioxidant and ACE Inhibition Assays
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The food-grade enzyme Alcalase 2.4 L was provided by Novozymes (Bagsvaerd, Denmark). 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), pyrocatechol violet, ferrozine, angiotensin-converting enzyme (ACE) and hippuryl-histidyl-leucine (HHL) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cu(NO3)2, Mg(NO3)2, Mn(NO3)2, Fe(NO3)3, NaNO3 KNO3, Ni(NO3)2 and Cr(NO3)3 Pb(NO3)2 and Cd(NO3)2 solutions were purchased from Merck (Darmstadt, Germany). All other chemicals were of analytical grade.
3
Reagents for Antioxidant and Cytotoxicity Assays
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Potassium ferricyanide, ferrous sulfate II, trichloroacetic acid, and sulfuric acid were purchased from Merck (Darmstadt, Germany). Nitro blue tetrazolium (NBT), monosaccharides, diaminoethanetetraacetic acid (EDTA), D-glucose, acid gallic, ascorbic acid, bovine serum albumin protein, ascorbic acid, methionine, 3-(4,5-dimethylthiazolyl-2)-2,5- diphenyltetrazolium bromide (MTT), pyrocatechol violet, riboflavin, ammonium molybdate, and tert-butyl hydrogen peroxide (t-BOOH) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Sodium bicarbonate, nonessential amino acids, and phosphate-buffered saline (PBS) were purchased from Invitrogen Corporation (Burlington, ON, Canada). Dulbecco's Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were obtained from CULTILAB (Campinas, SP, Brazil). Penicillin and streptomycin were obtained from Gibco (Fort Worth, TX, USA). All other solvents and chemicals were of analytical grade from Synth (Diadema, SP, Brazil).
4
Copper Chelation Evaluation of Blood Plasma Hydrolysates
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The Cu chelation ability of blood plasma hydrolysates was evaluated according
to Kong and Xiong (2006) (link) with a few
adjustments. The blood plasma hydrolysates (1 mL) were mixed with 1 mL of
0.2 mM CuSO4 in a centrifuge tube before being incubated at a
standard temperature for 5 min. The mixture was then treated with 1 mL of
11.3% trichloroacetic acid (TCA) solution before centrifugation at
2,500×g for 10 min. Then, the supernatant (2 mL) was mixed with 1 mL
of 10% pyridine and 20 μL of 0.1% pyrocatechol violet (Sigma-Aldrich)
in a test tube before being incubated at room temperature for 5 min. The
supernatant absorbance of the test samples was examined at 632 nm after
centrifuging them at 2,500×g for 10 min.
to Kong and Xiong (2006) (link) with a few
adjustments. The blood plasma hydrolysates (1 mL) were mixed with 1 mL of
0.2 mM CuSO4 in a centrifuge tube before being incubated at a
standard temperature for 5 min. The mixture was then treated with 1 mL of
11.3% trichloroacetic acid (TCA) solution before centrifugation at
2,500×g for 10 min. Then, the supernatant (2 mL) was mixed with 1 mL
of 10% pyridine and 20 μL of 0.1% pyrocatechol violet (Sigma-Aldrich)
in a test tube before being incubated at room temperature for 5 min. The
supernatant absorbance of the test samples was examined at 632 nm after
centrifuging them at 2,500×g for 10 min.
5
Comprehensive Materials for Biochemical Assays
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The 10, 40 and 147 kDa dextrans (Catalog number: 1179876, 1179865 and D-487); bovine serum albumin (BSA); sodium chloride; ferrozine; butylated hydroxyanisole (BHA); pyrocatechol violet; and ascorbic acid were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Potassium ferricyanide, ferrous sulfate (II), ethylenediaminetetraacetic acid (EDTA), Gallic acid, ammonium molybdate, hydrogen peroxide 30%, acetic acid, Folin–Ciocalteu phenol reagent, ethanol and sulfuric acid were obtained from Merck (Darmstadt, Germany). Culture media components Minimum essential Dulbecco’s modified Eagle medium (DMEM), l -Glutamine, sodium pyruvate, sodium bicarbonate, non-essential amino acids, fetal bovine serum and phosphate buffered saline (PBS) were acquired from Invitrogen Corporation (Burlington, ON, Canada). All other reagents and solvents were of analytical degree.
6
Antiophidic Bioactive Compounds Extraction
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Luteolin, orientin, isoorientin, vitexin, isovitexin, D-glicose, gallic acid, Cremophor® EL (polyoxyl 35 castor oil), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), Bothrops jararaca snake venom, bovine fibrinogen, bovine serum albumin, ascorbic acid, pyrocatechol violet and ferrozine were purchased from Sigma-Aldrich (St. Louis, MO, USA). All reagents of sodium dodecilsuphate polyacrylamide gel electrophoresis were purchased from GE Healthcare (Piscataway, NJ, USA). All reagents used for cell culture procedures were purchased from Cultilab (Campinas, SP, Brazil). All other reagents and solvents used were of analytical grade. The water used was purified by reverse osmosis.
7
Antioxidant Activity and Cytotoxicity Assays
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Ethylenediaminetetraacetic acid (EDTA), gallic acid, ascorbic acid, quercetin, methionine, pyrocatechol violet, riboflavin, and ammonium molybdate were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). Potassium ferricyanide, trichloroacetic acid, Folin-Ciocalteu reagent, and sulfuric acid were purchased from Merck (Darmstadt, Germany). DPPH (2,2-diphenyl-1-picrylhydrazyl) was purchased from Fluka (Seelze, Germany). Cupric sulfate was obtained from Chemical Kinetics. Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were obtained from CULTILAB (Campinas, SP, Brazil). Penicillin and streptomycin were obtained from Gibco (Fort Worth, TX, USA). All of the other reagents were of analytical grade.
8
Antioxidant Activity Assessment of Protein-based Ingredients
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Alcalase® 2.4 L, soy protein isolate (SPI), and maltodextrin (DE18‐20) were purchased from Novo (Nordisk, Bagsvaerd, Denmark), Jun Kai Chemical Co. (Zhengzhou, Henan Province, China), and Pooran Powder Sepahan Co. (Isfahan, Iran), respectively. WPC (80% protein) was obtained from Arla Co. (Denmark). Sodium nitroprusside, ferrozine 3‐(2‐pyridyl)‐5‐6‐diphenyl‐1,2,4‐triazine‐4′,4″ disulfonic acid sodium salt, pyrocatechol violet, naphthyl‐ethylene‐diamine‐dihydrochloride (NEDD), sulfanilamide, 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) diammonium salt (ABTS), and 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) were from Sigma–Aldrich Co. (St. Louis, MO, USA). Alpha‐deoxyribose was supplied from Fluka (Stockholm, Sweden), and all other chemicals used were from Merck Co. (Darmstadt, Germany).
9
Spectrophotometric Metal Detection Protocol
10
Reagents and Materials for Biochemical Assays
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Potassium ferricyanide, ferrous sulfate (II), ethylenediaminetetraAcetic acid (EDTA), gallic acid, ammonium molybdate, hydrogen peroxide 30%, Acetic acid, Folin–Ciocalteu phenol reagent, ethanol, and sulfuric acid were obtained from Merck (Darmstadt, Germany). Nitro blue tetrazolium (NBT), monosaccharides, EDTA, ascorbic acid, methionine, bovine serum albumin (BSA), pyrocatechol violet, ascorbic acid, and ammonium molybdate were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Sodium bicarbonate, culture media components (Minimum essential Dulbecco’s modified Eagle medium (DMEM)), non-essential amino acids, fetal bovine serum, sodium pyruvate, and phosphate buffered saline (PBS) were purchased from Invitrogen Canada Inc. (Burlington, ON, Canada). Acetonitrile (HPLC grade) purchased from Panreac® (São Paulo, Brazil). Acetic acid was provided by Vetec® (São Paulo, Brazil). Water was purified with a Milli-Q system (Millipore®, Bedford, MA, USA). All other solvents and chemicals were of analytical grade.
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