Log In Sign up
The largest database of trusted experimental protocols

Hmgb1

Manufactured by MyBioSource
Visit Supplier
Sourced in United States

HMGB1 is a protein that functions as a DNA-binding nuclear protein. It plays a role in the regulation of gene transcription.

Automatically generated - may contain errors

Lab products found in correlation

Hmgb1, by R&D Systems (2 mentions) Imark microplate reader, by Bio-Rad (1 mentions) Interleukin 6 (il 6), by MyBioSource (1 mentions) Interleukin 4 (il 4), by Abcam (1 mentions) Nucleosomes, by Roche (1 mentions) Fibrinogen, by Innovative Research (1 mentions) Ldh kit, by Takara Bio (1 mentions) Mip 2, by BioLegend (1 mentions) Osteopontin, by R&D Systems (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Kim 1, by R&D Systems (1 mentions) Quantity one software, by Bio-Rad (1 mentions) Ecl kit, by Beyotime (1 mentions) Gapdh, by Abcam (1 mentions)

Close spelling variants of this product

HMGB1 ELISA kit (5 citations) Human HMGB1 ELISA Kit (3 citations)

9 protocols using hmgb1

1

Quantifying Plasma Biomarkers in Humans and Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
The plasma levels of HMGB1, OPN and HA in both humans and mice were quantitated using the commercial sandwich enzyme-linked immunosorbent HMGB1 (MyBioSource, San Diego, CA, USA), OPN (R&D systems), and HA (R&D systems) ELISA kits following manufacturer’s instructions. The absorbance was read at 450 nm and the reading for each standard and sample subtracted the zero standard optical density (O.D.), and then the standard curve was created by reducing the data using computer software to generate a four-parameter logistic curve-fit. Except HMGB1 concentration measured in mice, which was expressed in pg/mL, all other concentrations were expressed in ng/mL.
Cao Y., Liu X, & Guo S.W. (2019). Plasma High Mobility Group Box 1 (HMGB1), Osteopontin (OPN), and Hyaluronic Acid (HA) as Admissible Biomarkers for Endometriosis. Scientific Reports, 9, 9272.
+ Open protocol
+ Expand
2

Quantifying Kidney Injury Markers and Inflammatory Mediators in Urine

Check if the same lab product or an alternative is used in the 5 most similar protocols
Kidney injury marker 1 in urine was determined by enzyme-linked immunoassay (ELISA) kit (MyBioSource, San Diego, CA) according to the supplier’s protocols. Additionally, the presence of relevant proinflammatory molecules, interleukin-6 (IL-6; MyBioSource, San Diego, CA) and high-mobility group box 1 (HMGB-1; MyBioSource, San Diego, CA) in urine samples were determined as per supplier’s protocol. Briefly, debris was removed from urine samples by centrifuging for 5 min at 3000 rpm. Subsequently, 100 μL of standards and urine samples were incubated in the precoated ELISA plate for 1 h. After washing, 100 μL of biotinylated detection antibody was added to the plates and incubated for 1 h. Plates were washed and 100 μL of avidin-HRP working solution was added for 1 h. Following a final wash, substrate reagent was added to the plate and color was developed. Plates were read at 450 nm using an iMark microplate reader (BioRad, Hercules, CA). Concentrations of the target proteins in the samples were determined from the standard curve.
Bhattacharjee R.N., Jackson A., Ruthirakanthan A., Juriasingani S., Levine M.A., Jiang L., Patel R., Richard-Mohamed M., Forrest S., Ravichandran S., Sener A, & Luke P.P. (2022). The Impact of Nutritional Supplementation on Donor Kidneys During Oxygenated Ex Vivo Subnormothermic Preservation. Transplantation Direct, 8(10), e1382.
+ Open protocol
+ Expand
3

Multiplex Biomarker Profiling in Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols
A panel of 26 biomarkers was measured in all plasma samples. Concentrations of HMGB-1, IL-1β, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12p70, IL-17-A, nucleosomes, Reg3A, ST2, TGF-β1 and TNFα were measured using ELISA kits (all from R&D Systems, Minneapolis, MN, except for HMGB-1 [MyBioSource, San Diego, CA], IL-4 [Abcam, Cambridge, UK] and nucleosomes [Roche, Indianapolis, IN]), and concentrations of CD40L, gp130, IFN-γ, IL-2Rα, IL-6Rα, IL-15, IL-22, IL-23, E-selectin, syndecan-1, thrombomodulin and TNFRI were measured using customized, magnetic bead-based, multi- or monoplex assays (R&D Systems). The magnetic beads were analysed on a Luminex LX-200 instrument (R&D Systems).
von Stemann J.H., Gjærde L.K., Haastrup E.K., Minculescu L., Brooks P.T., Sengeløv H., Hansen M.B, & Ostrowski S.R. (2021). Cytokine autoantibodies are stable throughout the haematopoietic stem cell transplantation course and are associated with distinct biomarker and blood cell profiles. Scientific Reports, 11, 23971.
+ Open protocol
+ Expand
4

Multiplex ELISA Cytotoxicity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse TNF-α, IL-6, MIP-2, KC (Biolegend), Fibrinogen (Innovative Research), and HMGB1 (MyBiosource) specific enzyme linked immunosorbent assays (ELISA) were performed according to the manufacturer’s instructions. LDH cytotoxicity assay was performed using an LDH kit (Clontech) according to the manufacturer’s instructions.
Kolar S.L., Kyme P., Tseng C.W., Soliman A., Kaplan A., Liang J., Nizet V., Jiang D., Murali R., Arditi M., Underhill D.M, & Liu G.Y. (2015). Group B Streptococcus evades host immunity by degrading hyaluronan. Cell host & microbe, 18(6), 694-704.
+ Open protocol
+ Expand
5

Quantifying Plasma Biomarkers in Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following the manufacturer’s instruction, the plasma levels of hyaluronic acid
(HA), high mobility group box 1 (HMGB1), and osteopontin were measured using HA
(R&D Systems, Minneapolis, MN), HMGB1 (MyBioSource, San Diego, CA),
osteopontin (R&D Systems), and ELISA kits, respectively.
Zhu B., Zhang C., Shen X., Chen C., Chen X., Lu Y., Chen Y, & Guo M. (2023). Protective Effects of Resveratrol Against Adenomyosis in a Mouse Model. Dose-Response, 21(1), 15593258231164055.
+ Open protocol
+ Expand
6

Plasma Biomarker Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood samples were centrifuged at 2500×g for 15 min, and plasma was collected for the measurements of blood urea nitrogen (ARBOR Assays), creatinine (ARBOR Assays), KIM-1 (R&D Systems), NGAL (R&D Systems), IL-6 (Thermo Fisher Scientific), and HMGB-1(MyBioSource, Inc.)
Meng Q., Tian X., Li J., Pruekprasert N., Dhawan R., Holz G.G, & Cooney R.N. (2022). GTS-21, a selective alpha7 nicotinic acetylcholine receptor agonist, ameliorates diabetic nephropathy in Leprdb/db mice. Scientific Reports, 12, 22360.
+ Open protocol
+ Expand
7

Spinal Protein Extraction and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spinal proteins were collected and purified with a protein extraction kit according to the manufacturer’s instructions (KC-415, KangChen, Shanghai, China). After electrophoresis and transfer, the proteins were incubated overnight at 4 °C with primary antibodies against HMGB1 (MyBioSource, CA, USA) and TLR3 (Abcam, Cambridge, MA, USA). GAPDH (Abcam, Cambridge, MA, USA) was used as the control. The protein bands were visualized with an ECL kit (Biyuntian, Beijing, China) and quantified using the Quantity One software (Bio-Rad Laboratories, Milan, Italy).
Li X.Q., Chen F.S., Tan W.F., Fang B., Zhang Z.L, & Ma H. (2017). Elevated microRNA-129-5p level ameliorates neuroinflammation and blood-spinal cord barrier damage after ischemia-reperfusion by inhibiting HMGB1 and the TLR3-cytokine pathway. Journal of Neuroinflammation, 14, 205.
+ Open protocol
+ Expand
8

Quantitative Analysis of Inflammatory Mediators

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quantitative analysis of inflammatory mediators HMGB1, SDC-1, and C3a in serum samples was performed using commercial enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s recommendations. The ELISA kits of HMGB1 (IBL International, Cat# 30164033), SDC-1 (Cloud-Clone Corporation, Cat# SEB966Po), and C3a (MyBioSource, Cat# MBS2509360) were used for measuring HMGB1, SDC-1, and C3a, respectively.
Young M.D., Cancio T.S., Thorpe C.R., Willis R.P., Snook J.K., Jordan B.S., Demons S.T., Salinas J, & Yang Z. (2023). Circulatory HMGB1 is an early predictive and prognostic biomarker of ARDS and mortality in a swine model of polytrauma. Frontiers in Immunology, 14, 1227751.
+ Open protocol
+ Expand
9

Characterizing Cytokine Secretion in Cancer Immunotherapy

Check if the same lab product or an alternative is used in the 5 most similar protocols
The concentration of cytokines secreted by R2016-treated tumor cells was measured using commercial ELISA kits for TGF-β1, IL-10, (eBioscience) and HMGB1 (MyBioSource, Inc., San Diego, CA, USA). To define the R2016-treated cultured-DC character, secretion of IL-12, IFN-γ, IL-10, 1L-6, and TGF-β1 was measured by ELISA kit (eBioscience).
Son K.J., Choi K.R., Ryu C.K., Lee S.J., Kim H.J, & Lee H. (2017). Induction of immunogenic cell death of tumors by newly synthesized heterocyclic quinone derivative. PLoS ONE, 12(3), e0173121.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!