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Eif3c

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EIF3c is a protein that is part of the eukaryotic translation initiation factor 3 (eIF3) complex. The eIF3 complex plays a crucial role in the initiation of protein synthesis by mediating the recruitment of the 40S ribosomal subunit to the mRNA. EIF3c is one of the core subunits of the eIF3 complex and is essential for its proper functioning.

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Lab products found in correlation

Pcbp2, by Abnova (1 mentions) Anti gadph, by Proteintech (1 mentions) Zg003, by Thermo Fisher Scientific (1 mentions) Molecular imager chemidoc xrs, by Bio-Rad (1 mentions) β actin, by Abcam (1 mentions) Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions)

2 protocols using eif3c

1

Protein Immunoblot Detection Assay

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eIF4G2 (Bethyl laboratories, A302-239A), CBP80 (Bethyl laboratories, A301-793A), eIF3c (Santa Cruz Biotechnology, sc-74507), PCBP2 (Abnova, H00005094-M05), anti-GADPH (Proteintech Group Inc, 10494-1-AP; or ZG003, Invitrogen), β-actin (Abcam, ab8229), and anti-RPSA antibodies were raised in mouse against full-length His6-tagged human RPSA protein expressed in E. coli. Western blots were exposed to Kodak X-ray film or visualized via a Bio-Rad ChemiDoc XRS+ Molecular Imager.
Smirnova V.V., Shestakova E.D., Bikmetov D.V., Chugunova A.A., Osterman I.A., Serebryakova M.V., Sergeeva O.V., Zatsepin T.S., Shatsky I.N, & Terenin I.M. (2019). eIF4G2 balances its own mRNA translation via a PCBP2-based feedback loop. RNA, 25(7), 757-767.
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2

Immunoblotting Analysis of eIF3c

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Proteins were separated by SDS‐PAGE and transferred to nitrocellulose membranes (Bio‐Rad, Hercules, CA, USA), after which the membranes were blocked in PBS/Tween‐20 containing 5% non‐fat milk. Then the membranes were probed with primary antibodies against eIF3c (Santa Cruz Biotechnology, Santa Cruz, CA, USA) overnight at 4°C. Immune‐reactive bands were detected using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, Waltham, MA, USA).
Li T., Li S., Chen D., Chen B., Yu T., Zhao F., Wang Q., Yao M., Huang S., Chen Z, & He X. (2017). Transcriptomic analyses of RNA‐binding proteins reveal eIF3c promotes cell proliferation in hepatocellular carcinoma. Cancer Science, 108(5), 877-885.
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