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Living image software 4.4

Manufactured by PerkinElmer
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Sourced in United States, Germany

Living Image software (version 4.4) is an integrated imaging platform developed by PerkinElmer for quantitative in vivo and ex vivo optical imaging. The software provides tools for image acquisition, processing, and analysis to support preclinical research applications.

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Lab products found in correlation

Ivis lumina xr system, by PerkinElmer (1 mentions)

2 protocols using living image software 4.4

1

In Vivo Fluorescence Imaging of GW1516 Nanoparticles

Cited 1 time
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GW1516@NP-OPN was injected into mouse tail veins.
The same amount of Cy 5.5 GW1516@NP was administered to the control
group mice. At 675 nm excitation, the 695 nm emission from the bilateral
carotid arteries was collected at 2, 6, 18, and 36 h after injection
with the IVIS Lumina XR System (Caliper Life Sciences, Hopkinton,
USA). The animals’ carotid arteries and aorta were excised
after fluorescence imaging. Following this, Living Image software
(4.4) (Caliper Life Sciences, Hopkinton, USA) was used to analyze
signal intensities as described previously.33 (link)
Huang X., Zhang Y., Zhang W., Qin C., Zhu Y., Fang Y., Wang Y., Tang C, & Cao F. (2022). Osteopontin-Targeted and PPARδ-Agonist-Loaded Nanoparticles Efficiently Reduce Atherosclerosis in Apolipoprotein E–/– Mice. ACS Omega, 7(33), 28767-28778.
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2

Quantification of Bioluminescence Imaging

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Quantification of BLI signal was performed as previously described52 (link). The Living Image software 4.4 (Caliper Life Sciences, Mainz, Germany) was used for data acquisition and quantification of light emission using a scale with a minimum of 1.8×104 photons per second per cm2 per solid angle of one steradian (sr). Different regions of interest (ROI) were defined and signals were considered positive, when light emission exceeded background in each ROI. Background was measured in mice harboring GLuc negative leukemias. A ROI covering the entire animal was used (background 4×106 photons per second). As an exception to determine early engraftment, a small ROI covering the femurs was used (background 6×104 photons per second), as light emission became visible there first. Overt leukemia was considered above 1010 photons per second using the ROI covering the entire animal.
Carlet M., Völse K., Vergalli J., Becker M., Herold T., Arner A., Senft D., Jurinovic V., Liu W.H., Gao Y., Dill V., Fehse B., Baldus C.D., Bastian L., Lenk L., Schewe D.M., Bagnoli J.W., Vick B., Schmid J.P., Wilhelm A., Marschalek R., Jost P.J., Miething C., Riecken K., Schmidt-Supprian M., Binder V, & Jeremias I. (2021). In vivo inducible reverse genetics in patients’ tumors to identify individual therapeutic targets. Nature Communications, 12, 5655.
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