Log In Sign up
The largest database of trusted experimental protocols

Ventana immunostainer

Manufactured by Abcam
Visit Supplier

The Ventana immunostainer is a fully automated system designed for immunohistochemistry (IHC) and in situ hybridization (ISH) assays. It automates the staining process, including reagent application, incubation, and slide washing, to provide consistent and reproducible results.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit anti cd68 antibody, by Abcam (2 mentions) Ventana immunostainer, by Roche (1 mentions) Imagescope software, by Leica Biosystems (1 mentions)

2 protocols using ventana immunostainer

1

Histological Analysis of Liver Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hematoxylin and eosin staining was performed on 5 μm sections from the paraffin-embedded tissue blocks for conventional light microscopy analysis. For CD68 staining, the liver sections were de-paraffinised and immunostained with rabbit anti-CD68 antibody (Abcam) using the automated Ventana immunostainer, according to manufacturer's recommendation. For CD3 staining, the liver sections were de-paraffinised and immunostained with rabbit monoclonal anti-CD3 antibody (Ventana Medical Systems, Inc) using the automated Ventana immunostainer, according to manufacturer's recommendation. For Sirius Red staining, liver sections were de-parrinised and stained using standard protocol.
Giles D.A., Moreno-Fernandez M.E., Stankiewicz T.E., Cappelletti M., Huppert S.S., Iwakura Y., Dong C., Shanmukhappa S.K, & Divanovic S. (2016). Regulation of Inflammation by IL-17A and IL-17F Modulates Non-Alcoholic Fatty Liver Disease Pathogenesis. PLoS ONE, 11(2), e0149783.
+ Open protocol
+ Expand
2

Histopathological Analysis of Liver

Check if the same lab product or an alternative is used in the 5 most similar protocols
Liver was collected and fixed in 10% buffered formalin solution overnight, followed by paraffin embedding. Hematoxylin and eosin staining was performed on 5μm sections from the paraffin-embedded tissue blocks for conventional light microscopy. For CD68 staining, liver sections were deparaffinized and immunostained with rabbit anti-CD68 antibody (Abcam, Cambridge, MA) using the automated Ventana immunostainer, according to manufacturer’s recommendation. The cell count was performed on scanned slides using Imagescope software (Leica Biosystems, Buffalo Grove, IL).
Kaplan J.M., Nowell M., Lahni P., Shen H., Shanmukhappa S.K, & Zingarelli B. (2016). Obesity enhances sepsis induced liver inflammation and injury in mice. Obesity (Silver Spring, Md.), 24(7), 1480-1488.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!