Leptin quantikine elisa kit

Manufactured by R&D Systems

Sourced in United States

The Leptin Quantikine ELISA kit is a quantitative sandwich enzyme immunoassay designed for the measurement of human leptin levels in serum, plasma, and cell culture supernatants. The kit uses a monoclonal antibody specific for leptin that has been pre-coated onto a microplate.

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Lab products found in correlation

Powerwave xs, by Agilent Technologies (1 mentions) Proteome profiler human xl cytokine array kit, by R&D Systems (1 mentions) Mrp300, by R&D Systems (1 mentions) Mob00, by R&D Systems (1 mentions) 2030 multilabel reader victor x5, by PerkinElmer (1 mentions) Rat tnf α quantikine elisa kit, by R&D Systems (1 mentions) Dc protein assay reagent, by Bio-Rad (1 mentions) Ultra sensitive elisa assay, by DRG International (1 mentions)

4 protocols using leptin quantikine elisa kit

Quantifying Adipokine Secretion in Adipose Tissue

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Leptin concentration in adipose conditioned media and human serum was quantified with the Leptin Quantikine ELISA kit from R&D Systems. Total adiponectin concentrations were similarly measured with the Total Adiponectin Quantikine ELISA kit from R&D Systems. Colorimetric determinations were made with a Powerwave XS plate reader (Bio-Tek).
Adipose explant conditioned media was assayed with the Proteome Profiler Human XL Cytokine Array Kit from R&D Systems according the manufacturer’s protocol. Resulting blots were exposed to film, which were scanned with a flatbed scanner and pixel intensity was measured with ImageJ. The leptin concentration quantified by ELISA was used to normalize pixel intensity to molar concentration in order to allow semi-quantitative determination of other adipokine concentrations.

Esper R.M., Dame M., McClintock S., Holt P.R., Dannenberg A.J., Wicha M.S, & Brenner D.E. (2015). Leptin and Adiponectin Modulate the Self-renewal of Normal Human Breast Epithelial Stem Cells. Cancer prevention research (Philadelphia, Pa.), 8(12), 1174-1183.

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ELISA-based Adiponectin and Leptin Quantification

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The Ad and leptin plasmatic concentration were measured by using the mouse Ad/Acrp30 Quantikine ELISA Kit and the mouse Leptin Quantikine ELISA Kit, according to the manufacturer’s instructions (Ad: MRP300; Leptin: MOB00, R&D Systems, Minneapolis, MN, United States).

Pierard M., Tassin A., Conotte S., Zouaoui Boudjeltia K, & Legrand A. (2019). Sustained Intermittent Hypoxemia Induces Adiponectin Oligomers Redistribution and a Tissue-Specific Modulation of Adiponectin Receptor in Mice. Frontiers in Physiology, 10, 68.

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Plasma and Analyte ELISA Assays

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Activities of plasma and analyte concentrations were determined using enzyme-linked immunosorbent assay (ELISA) commercial kits according to manufacturer protocols [9 (link), 32 (link)]. The NFκβ p65 was determined using rat NFκβ p65 ELISA kit by Elabscience, China. The TNFα, leptin and adiponectin were determined using rat TNFα Quantikine ELISA kit, Leptin Quantikine ELISA kit and Adiponectin Quantikine ELISA kit respectively by R&D Systems, USA. The analyte concentrations were analysed using Perkin Elmer 2030 Multilabel Reader Victor™ X5.

Reshidan N.H., Abd Muid S, & Mamikutty N. (2019). The effects of Pandanus amaryllifolius (Roxb.) leaf water extracts on fructose-induced metabolic syndrome rat model. BMC Complementary and Alternative Medicine, 19, 232.

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Metabolic Biomarkers in Mice

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Blood glucose concentrations were determined in samples from lateral tail vein using a glucose sensor (Bayer, Germany). Post‐absorptive plasma insulin levels were measured by an ultra‐sensitive ELISA assay (DRG Instruments GmbH, Germany). The plasma concentrations of cytokines (Mouse/Rat GDF15, FGF21, and Leptin Quantikine ELISA Kit, R&D Systems, USA) were determined by enzyme‐linked immunosorbent assays (ELISA) following manufacturer instructions. Mouse GDF15 (mGDF15) protein levels were analyzed in skeletal muscle lysates of quadriceps from 20‐week‐old male WT and Ucp1‐TG animals. Muscle tissue was homogenized in hypotonic buffer (10 mM Tris pH 7.4, 0.02% Triton X‐100, 1× COMPLETE EDTA‐free), GDF15 was measured using Mouse/Rat GDF15 ELISA Kit (R&D Systems), and values were normalized to total protein concentration (DC™ Protein Assay Reagent [#500‐0114, BioRad]).

Ost M., Igual Gil C., Coleman V., Keipert S., Efstathiou S., Vidic V., Weyers M, & Klaus S. (2020). Muscle‐derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress. EMBO Reports, 21(3), e48804.

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