Enzyme-linked immunosorbent assay kits for rat insulin, total bilirubin, blood urea nitrogen, creatinine, total cholesterol, triglyceride, free fatty acid, and leptin were purchased from Randox Laboratories (Crumlin Co., Antrim, UK). Biochemical analyses were performed according to the manufacturer’s protocols.
Total bilirubin
Manufactured by Randox
Sourced in United Kingdom
The Total Bilirubin assay is a laboratory test used to measure the total amount of bilirubin in the blood. Bilirubin is a yellowish pigment produced during the breakdown of red blood cells. This test is commonly used to assess liver function and detect potential issues related to bilirubin metabolism.
Automatically generated - may contain errors
Lab products found in correlation
Creatinine, by Randox (4 mentions)
1 chloro 2 4 dinitrobenzene cdnb 5 5 dithiobis 2 nitrobenzoic acid dtnb epinephrine, by Merck Group (3 mentions)
Glutathione (gsh), by Merck Group (3 mentions)
Hydrogen peroxide h2o2, by Merck Group (3 mentions)
Direct bilirubin, by Randox (2 mentions)
Gallic acid, by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Total cholesterol, by Randox (1 mentions)
Total protein, by Randox (1 mentions)
Alanine aminotransferase, by Randox (1 mentions)
Alkaline phosphatase, by Randox (1 mentions)
Gamma glutamyl transferase, by Randox (1 mentions)
6 protocols using total bilirubin
1
Biomarker Evaluation in Rats
2
Blood Serum Collection and Analysis
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The procedure described by Yakubu and Akanji (2011) was adopted for the preparation of serum. The animals were sacri ced by cervical dislocation and the blood collected by direct heart punctured into EDTA sample bottles and spinned at 3000rpm for 20mins. The serum of both control and treated animals were carefully aspirated with Pasteur pipette into sample bottles for the various biochemical assays which are Alkaline phosphate (ALP), Aspartate aminotranferase (AST), Alanine amino tranferase (ALT), urea, total bilirubin, direct bilirubin and creatinine were products of Randox Laborites Limited, United Kingdom.
3
Methotrexate-Induced Oxidative Stress
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Methotrexate tablets is a product of West Coast Pharmaceutical Works Ltd, Gota, Ahmedabad, India; Gallic acid, Glutathione, 1-chloro-2,4-dinitrobenzene (CDNB),5,5 -dithio bis-2-nitrobenzoic acid (DTNB), epinephrine, and hydrogen peroxide (H2O2), were obtained from Sigma® Chemical Company, London, UK; Assay kits for alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl transferase (GGT), alkaline phosphatase (ALP), urea, Creatinine, total bilirubin were purchased from Randox® Laboratories Ltd. (Antrim, UK). All other reagents used were of analytical grade and of highest purity.
4
Melphalan-Induced Hepatotoxicity Study
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Melphalan tablets were a product of Excella GmbH, Nürnberger, Germany. Quercetin, glutathione, 1-chloro-2,4-dinitrobenzene (CDNB), 5,5-dithiobis-2-nitrobenzoic acid (DTNB), epinephrine, and hydrogen peroxide (H2O2) were all purchased from Sigma Chemical Company (London, UK). Kits for alanine transferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma glutamyl transpeptidase (GGT), urea, creatinine, and total bilirubin were products of Randox laboratories Ltd. (Antrim, UK). All other reagents used were of analytical grade and of the highest purity.
5
Cyclophosphamide Hepatotoxicity Evaluation
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Cyclophosphamide tablet is a product of West Coast Pharmaceutical Works Ltd, Gota, Ahmedabad, India. Glutathione, 1-chloro-2,4-dinitrobenzene (CDNB), 5,5-dithiobis-2-nitrobenzoic acid (DTNB), epinephrine, and hydrogen peroxide (H2O2) were all purchased from Sigma Chemical Company (London, UK). Kits for alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), Urea, Creatinine and total Bilirubin were obtained from Randox laboratories ltd. (Antrim, UK). All other reagents used were of analytical grade and of highest purity.
6
Comprehensive Biochemical Profiling Protocol
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Total cholesterol, alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), total bilirubin and direct bilirubin, total protein, albumin, urea, creatinine, calcium, sodium, chloride, potassium, and bicarbonate test kits were purchased from Randox Laboratory (United Kingdom).
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