Whatman 903 paper

Manufactured by Cytiva

Sourced in United Kingdom

Whatman 903 paper is a laboratory filter paper designed for the collection and storage of dried biological samples, such as newborn screening samples. It is a high-quality, high-purity cellulose-based paper that meets the requirements for collecting and preserving blood spot samples.

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Whatman, by Cytiva (5 mentions) Electronic scale, by Seca (1 mentions) Flexible tape measure, by Seca (1 mentions)

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5 protocols using whatman 903 paper

Collection of DBS Samples from High-Risk MSM

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With proper informed consent, all DBS samples were collected by a local NGO, namely, AIDS Concern in Hong Kong. In total, 474 DBS samples were collected between March 2010 and April 2011 from sexually active high-risk MSM groups at local MSM venues, including bars, saunas, and clubs, as well as at VCT offices of AIDS Concern. Dried blood spots were collected by spotting five blood drop samples (50 μl each) onto each of five rings on a Whatman 903 paper (Whatman Inc., Piscataway, NJ, USA). After the spots were air-dried thoroughly at room temperature, each Whatman 903 paper was bar-coded and sealed in a plastic bag for DNA extraction.

Liang J., Liu L., Cheung M., Lee M.P., Wang H., Li C.H., Chan C.C., Nishiura K., Tang X., Tan Z., Peng J., Cheung K.W., Yam W.C, & Chen Z. (2015). Community-Based HIV-1 Early Diagnosis and Risk Behavior Analysis of Men Having Sex with Men in Hong Kong. PLoS ONE, 10(4), e0125715.

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Comprehensive Health Assessment Protocol

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Interviews lasting 2–3 hours were conducted in participants’ homes. The visit included a questionnaire in two parts: household and socioeconomic data then individual interview data; followed by anthropometric, physical and cognitive functioning assessments plus blood sample collection in the form of capillary blood sample and “dried blood spots” (DBS). Data were captured on laptop computers using a Computer Assisted Personal Interview (CAPI) program.
Data collected included blood pressure (Omron M6W automated cuff; Omron, Kyoto, Japan), weight (Genesis Growth Management Electronic Scale; Johannesburg, South Africa); height using a sensor with infrared measurement; waist and hip circumferences with a flexible tape measure (SECA, Hamburg, Germany). Blood drops were used to measure glucose (Caresense© N Monitor, Seoul, Korea) and individual lipid levels (Cardiocheck© PA Silver version; Indianapolis, Indiana, USA) in point-of-care machines. The DBSs were collected via finger prick on Whatman 903 ^™ paper (Whatman, Buckinghamshire, UK) and used to measure HIV-status and high sensitivity C-reactive protein (hsCRP). Three blood pressure readings (systolic and diastolic) were obtained with two minutes between each reading and the mean blood pressure was calculated using the average between the second and third reading¹⁴.

Jardim T.V., Reiger S., Abrahams-Gessel S., Crowther N.J., Wade A., Gómez-Olivé F.X., Salomon J., Tollman S, & Gaziano T.A. (2017). Disparities in Management of Cardiovascular Disease in Rural South Africa: Data from the HAALSI (Health and Aging in Africa: Longitudinal Studies of INDEPTH Communities) Study. Circulation. Cardiovascular quality and outcomes, 10(11), e004094.

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Inter-Rater Agreement in Newborn Screening

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Blood from a heel prick was collected onto Whatman 903 paper (Whatman International Ltd, Chalfont St Giles, United Kingdom) for NBS. Fifty DBS were used to perform ten inter-rater studies to assess the agreement on the classification of the specimen’s quality in the period between 2015 and 2020. Of the selected DBS, 22 had an acceptable quality and 28 were unacceptable (6 scant and 22 non-optimal specimens). Twenty-five DBS were used to conduct four inter-rater studies to assess the agreement on the assignment of Hb patterns between 2017 and 2020. For these studies, the following cases were selected: 2 unaffected, 15 affected (3 beta thalassaemia major, 7 sickle cell disease, 3 alpha thalassaemia, 1 Hb E disease and 1 Hb D disease) and 8 carriers of Hb variants.
Specific data about the samples included in each study and the number of observers who participated are shown in Table 1.

Guiñón L., Soler A., López R.M., Pajares S., de Aledo J.M., Argudo-Ramírez A., Marín J.L., García-Villoria J., Sahuquillo Á, & Alvarez L. (2022). Inter-rater reliability assessment for the new-born screening quality assurance. Biochemia Medica, 32(3), 030901.

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G6PD Study in Myanmar Military

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A cross-sectional survey was conducted in Nay Pyi Taw, Myanmar. A total of 772 blood samples were collected from military personnel and their families residing in military camps in Tatkon (160 samples), Ottara Thiri (332 samples), and Zayar Thiri (280 samples) (Fig. 1). The Human Subjects protocol for this study was approved by the Ethical Review Committee of the Faculty of Tropical Medicine, Mahidol University, Thailand (MUTM2016-036-01), and the Institutional Review Board of the Defence Services Medical Research Centre (DSMRC), Myanmar (IRB/2016/60). Written informed consent was obtained before blood collection. Three millilitres of blood were obtained by venipuncture and collected in an EDTA tube. Blood was stored in a cooler box with ice packs during transportation from the sites to the DSMRC laboratory. Samples were processed for G6PD testing within 24 h after blood collection. An aliquot of the blood was spotted on Whatman 903 paper for later analysis of G6PD mutations.
Fig. 1

Location of the five study sites from Tatkon, Ottara Thiri, and Zayar Thiri Township, Nay Pyi Taw division area

Aung T.H., Suansomjit C., Tun Z.M., Hlaing T.M., Kaewkungwal J., Cui L., Sattabongkot J, & Roobsoong W. (2023). Prevalence of G6PD deficiency and diagnostic accuracy of a G6PD point-of-care test among a population at risk of malaria in Myanmar. Malaria Journal, 22, 143.

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Newborn Screening for Congenital Adrenal Hyperplasia in Victoria, Australia

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The NBS program for the State of Victoria, Australia is located within the Royal Children’s Hospital Melbourne campus at the Victorian Clinical Genetics Services (VCGS). The NBS laboratory services around 90 maternity service providers, testing approximately 80,000 newborns annually, including for CAH since 2022 [26 (link)]. The dried bloodspot (DBS) sample is usually collected via a capillary heel prick onto Whatman 903 paper when the baby is 36 to 72 h of age [27 ,28 (link)]. On arrival to the laboratory, one 3.2 mm spot is punched from the card for the first tier 17OHP immunoassay which is analysed using the Perkin–Elmer (Turku, Finland) GSP^® Neonatal 17OHP kit. For results ≥20.0 nmol/L blood (approximating the 99th centile), another 3.2 mm punch is taken for second-tier LC–MS/MS steroid analysis.

Greaves R.F., Kumar M., Mawad N., Francescon A., Le C., O’Connell M., Chi J, & Pitt J. (2023). Best Practice for Identification of Classical 21-Hydroxylase Deficiency Should Include 21 Deoxycortisol Analysis with Appropriate Isomeric Steroid Separation. International Journal of Neonatal Screening, 9(4), 58.

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