Goat anti rabbit igg h l

Manufactured by Wuhan Servicebio Technology

Sourced in China

Goat Anti-Rabbit IgG (H+L) is a secondary antibody used to detect and bind to rabbit primary antibodies. It is produced by immunizing goats with rabbit immunoglobulin and purifying the resulting antibodies.

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Lab products found in correlation

Rabbit igg polyclonal, by Wuhan Servicebio Technology (1 mentions) Hrp labeled goat anti rabbit igg h l, by Wuhan Servicebio Technology (1 mentions) Ethylene diamine tetraacetic acid (edta), by Wuhan Servicebio Technology (1 mentions) Image pro plus 6, by Media Cybernetics (1 mentions) Gb21303, by Wuhan Servicebio Technology (1 mentions) Gb11159 1, by Wuhan Servicebio Technology (1 mentions) Gb23303, by Wuhan Servicebio Technology (1 mentions) Confocal laser scanning microscope, by Olympus (1 mentions) Paraformaldehyde (pfa), by Solarbio (1 mentions) Fluorescence microscope, by Olympus (1 mentions) Hrp conjugated goat anti mouse igg h l, by Wuhan Servicebio Technology (1 mentions) Ipvh00010, by Merck Group (1 mentions) E bc k318 m, by Elabscience (1 mentions) Hc201, by Transgene (1 mentions) Eclipse c1, by Nikon (1 mentions)

5 protocols using goat anti rabbit igg h l

Histological Analysis of Wound Healing

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The wounded skin was excised and fixed with 4% paraformaldehyde (Solarbio, Beijing, China) for 24 h to prevent cell autolysis after death. Then, the sections were hydrated with running water for a certain period of time, followed by gradual dehydration with alcohol at different concentrations, embedding in paraffin and cutting into 5 μm sections. The sections were stained with hematoxylin-eosin (HE), and the histological changes of the wounds were visualized under a microscope.
Skin tissues were sectioned into 5 μm sections as above. For immunofluorescence double staining, sections were incubated overnight at 4°C with a mix of primary anti-PGP9.5 (GB11159-1, 1:1,000, Servicebio) and anti-GAP43 (bs-0154R, 1:4,000, Bioss) antibodies. Sections were incubated in Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303, 1: 300, Servicebio) or HRP conjugated Goat Anti-Rabbit IgG (H+L) (GB23303, 1:500, Servicebio) secondary antibody for 1 h at room temperature. Images were captured by laser scanning confocal microscope (Olympus, Japan), and the confocal software was used for acquisition of the data and merging of the digital images. Each antibody was validated separately prior to use in double immunofluorescence.

Zhu Z., Zhang X., Hao H., Xu H., Shu J., Hou Q, & Wang M. (2022). Exosomes Derived From Umbilical Cord Mesenchymal Stem Cells Treat Cutaneous Nerve Damage and Promote Wound Healing. Frontiers in Cellular Neuroscience, 16, 913009.

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Comprehensive Tissue Histology Protocols

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Hematoxylin and eosin (H&E) staining, immunohistochemical (IHC) staining and immunofluorescence staining were all conducted using standard techniques (Servicebio Co, China). Briefly, for the IHC, the slides were deparaffinized and antigen retrieval was then performed using a microwave oven in EDTA, pH = 8.0 (Servicebio, Wuhan, China). Primary antibodies of HIF-1α (Abcam, Rabbit IgG polyclonal, Cambridge, UK), CD31 (Servicebio, Rabbit IgG polyclonal), and NLRP3 (Servicebio, Rabbit IgG polyclonal) were applied overnight before HRP-labeled Goat Anti-rabbit IgG (H+L) (Servicebio) incubation for 50 min at room temperature. DAB was used as chromogens and slides were counterstained with hematoxylin before mounting. For the immunofluorescence staining, primary antibodies for CD31 (Servicebio, Rabbit IgG polyclonal) and α-SMA (Servicebio, mouse monoclonal 1A4) and Cy3 conjugated Goat Anti-rabbit IgG (H+L) (Servicebio) or Alexa Fluor^® 488-conjugated Goat Anti-mouse IgG (H+L) (Servicebio) were used for generating fluorescence staining. The stained sections were subjected and observed by microscopy (Olympus BX53, Tokyo, Japan). The average IOD of HIF-1α, CD31, and NLRP3 in five randomly selected areas for each group were calculated by using Imagepro Plus 6.0 (Media Cybernetics, Inc.).

Wang T., Meng J., Wang C., Wen T., Jia M., Ge Y., Xie L., Hao S., Liu J, & Xu H. (2019). Inhibition of Murine Breast Cancer Metastases by Hydrophilic As4S4 Nanoparticles Is Associated With Decreased ROS and HIF-1α Downregulation. Frontiers in Oncology, 9, 333.

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Immunofluorescence Assay for MKI67

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The indicated cells were seeded on six‐well plates at 2 × 10⁶ cells/well. Standard immunofluorescence procedures were carried out as a previous study.²² Rabbit anti‐MKI67 (Servicebio) antibody was used as the primary antibody. Goat anti‐Rabbit IgG (H + L) conjugated with Cy5 (Servicebio) was used as the secondary antibody. The cover slips were observed using a fluorescence microscope (Olympus). Blue represented the nucleus stained with DAPI, and red represented MKI67 protein.

Chen J., Wu S., Wang J., Han C., Zhao L., He K., Jia Y, & Cui M. (2023). MCM10: An effective treatment target and a prognostic biomarker in patients with uterine corpus endometrial carcinoma. Journal of Cellular and Molecular Medicine, 27(12), 1708-1724.

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Western Blot Analysis of DARS2 Protein

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Treated LUAD cells were extracted by RIPA lysis buffer (C1053, Beijing Pulilai Gene Technology Co., Ltd., China). The protein concentrations were measured by BCA method (E-BC-K318-M, Elabscience, China). 40 μg of protein was separated by 10% SDS-polyacrylamide gels. The protein samples were transferred onto PVDF membranes (IPVH00010, Millipore, USA). The membranes were blocked with 5% BSA for 2 h. Followed by incubation with primary antibodies, including Rabbit Anti-DARS2 (DF12593, Affinity, China, 1/1000) and Mouse Anti-β-Actin (HC201, TransGen Biotech, China, 1/2000). The membranes were washed with TBST, and then incubated with corresponding secondary antibodies, including HRP conjugated Goat Anti-Mouse IgG (H+L) (GB23301, Servicebio, China, 1/2000) and HRP conjugated Goat Anti-Rabbit IgG (H+L) (GB23303, Servicebio, China, 1/2000), at room temperature for 1 h. Finally, the proteins were visualized by an enhanced chemiluminescence (ECL) detection kit (RJ239676, Thermo Fisher Scientific, Inc., USA). We captured the chemiluminescent signal of protein bands using the Tanon-5200 chemiluminescence imaging system (Tanon, Shanghai, China). The optical density of protein bands was quantified by using ImageJ software (Image J Software Inc., USA).

Liu X.S., Yuan L.L., Gao Y., Ming X., Zhang Y.H., Zhang Y., Liu Z.Y., Yang Y, & Pei Z.J. (2023). DARS2 overexpression is associated with PET/CT metabolic parameters and affects glycolytic activity in lung adenocarcinoma. Journal of Translational Medicine, 21, 574.

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Nrf2 Immunohistochemistry in Retina

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Paraffin-embedded retina tissue sections were deparaffinized, hydrated, microwaved in Tris-EDTA buffer for antigen retrieval, and finally rinsed in PBS. After blocking with bovine serum albumin for 30 min to block endogenous peroxidase activity, the sections were incubated overnight with the rabbit anti-Nrf2 (1:200, Servicebio Biotechnology Co. Ltd., Wuhan, China). The negative control was incubated with PBS. After adequate washing, sections were coincubated with goat anti-rabbit IgG H&L (1:1,500, Servicebio Biotechnology Co. Ltd., Wuhan, China) for 2 h at room temperature and then stained with DAPI for 5 min. Images were acquired at 400× magnification using Ortho-Fluorescent Microscopy. (Nikon Eclipse C1, Nikon Instrument Inc., Japan) and five fields of view were randomly selected for each sample.

Xie T., Chen X., Chen W., Huang S., Peng X., Tian L., Wu X, & Huang Y. (2021). Curcumin is a Potential Adjuvant to Alleviates Diabetic Retinal Injury via Reducing Oxidative Stress and Maintaining Nrf2 Pathway Homeostasis. Frontiers in Pharmacology, 12, 796565.

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