A498 and 786-O cells were transfected with 10 nM miRNAs or siRNAs by reverse transfection. Cell proliferation was determined by XTT assay using a Cell Proliferation Kit II (Sigma-Aldrich, St. Louis, MO, USA). Cell migration was evaluated with wound healing assays. Cell invasion was analyzed using modified Boyden chambers containing Transwell-precoated Matrigel membrane filter inserts. These assays were performed as described previously [45 (link),46 (link),47 (link)].
Cell proliferation kit 2
Manufactured by Merck Group
Sourced in United States, France
The Cell Proliferation Kit II is a laboratory equipment product developed by Merck Group. It is designed to measure cell proliferation by quantifying the incorporation of a labeling substance into newly synthesized DNA. The kit provides the necessary reagents and protocols to perform this analysis.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Spectramax id3, by Molecular Devices (2 mentions)
Prestoblue cell viability reagent, by Thermo Fisher Scientific (2 mentions)
96 well plate, by Greiner (1 mentions)
Model 680 microplate reader, by Bio-Rad (1 mentions)
Lambda bio 20, by Beckman Coulter (1 mentions)
Luciferin, by GoldBio (1 mentions)
Glutamate assay kit, by Merck Group (1 mentions)
Lactate assay kit, by Cayman Chemical (1 mentions)
D3h8q, by Cell Signaling Technology (1 mentions)
6 diazo 5 oxo l norleucine, by Merck Group (1 mentions)
Palbociclib, by MedChemExpress (1 mentions)
Dual luciferase reporter assay system, by Promega (1 mentions)
Sybr green master mix, by Bio-Rad (1 mentions)
Oleanolic acid, by Merck Group (1 mentions)
Vegf elisa kit, by Thermo Fisher Scientific (1 mentions)
Cytofixtm fixation buffer, by BD (1 mentions)
Ursolic acid, by Merck Group (1 mentions)
Silver nitrate agno3, by Thermo Fisher Scientific (1 mentions)
Folin ciocalteu phenol reagent, by Merck Group (1 mentions)
45 protocols using cell proliferation kit 2
1
miRNA and siRNA Transfection Effects on Cell Proliferation, Migration, and Invasion
2
XTT-based Cell Viability Assay
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The ex vivo cell viability was determined using an XTT-based method (Cell Proliferation Kit II, Sigma-Aldrich, Saint-Quentin-Fallavier, France). Analyses were performed according to the supplier’s recommendations. Optical density was measured at 450 nm.
3
Metabolic Activity Quantification by XTT
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Metabolic activity of cells was assessed by the XTT assay (Cell Proliferation Kit II, Sigma) according to the manufacturer’s recommendations. Briefly, cells were seeded onto 96-well plates (Greiner) and incubated with the compounds for 12 h. H2O2 (1 mM) was used as a positive control. Absorbance was measured at 492 and 690 nm 4 h post addition of the XTT reagent.
4
Drug Sensitivity and Cell Proliferation Assay
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T24, T24/DOX, and T24/CIS (2 × 103 /well) in 96-well plates were allowed to attach overnight. For drug sensitivity analysis, T24 and T24/DOX cells were treated with DOX (0, 0.1, 1, 10, 20, and 100 μM) for 24 h, while T24 and T24/CIS cells underwent 24 h treatment with CIS (0, 10, 20, 40, and 80 μM). To determine the effect of NTX on cell proliferation, T24, T24/DOX, and T24/CIS cells were exposed to NTX (0, 2.5, 5, 10, 20, and 40 μM) for 24, 48, and 72 h. At the indicated time points, culture media were removed and the cells were washed gently with PBS. The prepared XTT working solution (Cell Proliferation Kit II, #11465015001, Sigma-Aldrich) was added to the cells, which were subsequently incubated for 4-24 h at 37 ℃. Finally, the optical density (OD) levels were determined by the Model 680 Microplate Reader (Bio-Rad, USA).
5
Macrophage Viability Assessment by XTT
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Macrophage viability was measured using tetrazolium salts (XTT), Cell Proliferation Kit II (Sigma-Aldrich) according to the manufacturer’s instructions. Cells were then cultured with EV-free medium for 24, 48 or 72h, after which the XTT assay was performed. Cell proliferation was spectrophotometrically quantified using a 96 wells plate reader at 450 nm. A decrease in optical density was analyzed by normalization against untreated cells with EV-free medium (control cells). All assays were prepared in triplicates.
6
Cell Viability Assay in 96-well Plate
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RAW264.7 and MC3T3-E1 cells were seeded in a 96-well plate at a density of 3.5 × 103 cells per well and then cultured for 48 h or 7 days with 2% serum (2% FBS or 1.25% FBS + 0.75% CT or PGPE mice serum). Cell viability was determined by an XTT-based method using Cell Proliferation Kit II (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s recommendations. The OD was determined at 450 nm.
7
Assessing Cell Viability and Nitric Oxide Synthesis
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After 24 h of stimulation, cell viability was assessed using an XTT assay, according to manufacturer’s instruction (Cell Proliferation Kit II, Sigma Aldrich, Buchs, Switzerland). Nitric oxide synthase activity was determined by measuring the release of NO2− in the cell culture supernatant as previously reported30 (link).
8
Evaluating pHLIP-miR-29a Effects on Cell Proliferation
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The effects of pHLIP-miR-29a on the proliferation of A549 and HT-29 cells were measured using a Cell Proliferation Kit II (XTT) (Sigma-Aldrich, Inc., St. Louis, MO, USA). The cells (5 × 104 /ml) were treated with pHLIP-miR-29a (0, 100, 250, or 500 nM) in 96-well plates for 48 h. Assays were performed in parallel using pHLIP-scr as a control. All treatments were performed at the indicated pH. After 48 h, the extent of cell growth was assessed using the Cell Proliferation Kit II (XTT). Briefly, XTT solution (50 μl) was added to each well and the cells were incubated for 2 h at 37 °C. Absorbance at 450 nm was then determined using a Lambda Bio-20 multiplate reader (Beckman Coulter, Brea, CA, USA). Cell proliferation was expressed as a percentage of that of control cells.
9
BMSC and DPSC Viability in Hydrogel
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Cultured BMSCs and hDPSCs were washed with PBS and then trypsinized using 0.5% trypsin EDTA solution (Sigma, St. Louis, MO, USA) then centrifuged at 1500 rpm for 5 minutes. The supernatant was removed, and cells were suspended in complete medium and 1×106 cells in 100 µL medium were acquired. The cells were then mixed with NC-CS/GP-21 hydrogel then 50 µL of the mixture were added to a 96-well plate (2 × 104 cells/well). For cells outside the hydrogel and negative control group, 50 µL of each were added directly to 96-well plates. All plates were then incubated at 37°C in 5% CO2. After 2 hours of incubation, 100 µL of growth medium was added into the wells, and then cells were incubated at 37°C in 5% CO2 for 21 days. Cell viability was detected using Cell Proliferation Kit II (Sigma, St. Louis, MO, USA) according to the manufacturer’s instructions. Absorbance was measured using a spectrophotometer at 490 nm with a reference wavelength at 650 nm. Experiments were carried out in four replicates and repeated twice before calculating the average viability.
10
XTT Cell Viability Assay
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The cell viability was investigated on an XTT-based method (Cell Proliferation Kit II, Sigma-Aldrich, Saint-Quentin-Fallavier, France). Experimental procedures were set according to the supplier’s recommendations. The optical density was measured at 450 nm.
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