Bxpc 3

Human pancreatic cancer cell lines (AsPC-1, BxPC-3, and MIAPaCa-2) were obtained from American Type Culture Collection (Manassas, VA, USA). MIAPaCa-2 cells were maintained in D-MEM medium (Sigma, St. Louis, MO, USA) supplemented with 5% fetal bovine serum (Sigma) in a humidified incubator maintained at 37°C with 5% CO₂. AsPC-1 and BxPC-3 cells were maintained in RPMI 1640 medium (Wako Pure Chemical Industries, Osaka, Japan) supplemented with 10% fetal bovine serum.

The WiDr, SW480, DLD-1, SW837, PANC-1, MIA PaCa-2, ASF 4-1, TIG-3-20, and KMST-6 cell lines were obtained from the Japanese Cancer Research Resources Bank. The SW48 cell line was from the Cell Resource Center for Biomedical Research of Tohoku University. The HT-29 and H9c2 cell lines were procured from the American Type Culture Collection. The BxPC-3 cell line was obtained from the European Collection of Authenticated Cell Cultures. RPMI-1640 medium (Wako Pure Chemicals Industries) used to culture SW48, WiDr, HT-29, SW480, DLD-1, SW837, and BxPC-3 cells; Eagle’s minimum essential medium (Wako) for MIA PaCa-2, PANC-1, ASF 4-1, and TIG-3-20 cells and Dulbecco’s minimum essential medium (Wako) for H9c2 cells. All media were supplemented with 10% (v/v) heat-inactivated FBS (Nichirei Biosciences, Tokyo, Japan), and cells were incubated under an atmosphere of 95% air and 5% CO₂ at 37°C. Testing for Mycoplasma contamination was performed using the activity of certain mycoplasmal enzymes (MycoAlert Mycoplasma detection kit; Lonza, Basel, Switzerland). The number of viable cells was assessed using the trypan blue dye exclusion test.

Human pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-1, MIA PaCa-2, PANC-1, and PSN-1) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). The cells were cultured in a humidified atmosphere of 5% CO₂ at 37 °C. RPMI 1640 (Wako) supplemented with 10% fetal bovine serum (FBS) was used for AsPC-1, BxPC-3, and PSN-1. On the other hand, DMEM (Wako, Osaka, Japan) supplemented with 10% FBS was used for MIA PaCa-2 and PANC-1, while IMDM (Gibco, Waltham, MA, USA) supplemented with 20% FBS was used for Capan-1. Exponentially growing cells were detached from the culture plates with trypsin and used in this study. The number of viable cells was determined using the trypan blue dye exclusion method.