The saturated and unsaturated lipid contents in the hippocampus were measured by Fourier Transform Infrared (FTIR) microscopy referring to previous method [39 (link)]. The brains were quickly frozen using cryogenic techniques and stored at −80 °C before being sliced into 12 μm sections using a CM1950 Cryo-microtome from Leica, Germany. The slices were observed on microscopy slides. The FTIR data were obtained with a Spotlight 400 FTIR spectrometer from PerkinElmer in the United States. The data were collected in transmission mode within the range of 4000 to 800 cm−1. We used a detector made of MCT with a Focal Plane Array (FPA) of 128 × 128, which was cooled using liquid nitrogen. Spectrum IMAGE 1.8 (PerkinElmer, MA, USA) was used to analyze the FTIR spectra. Chemical mapping was recorded for specific absorption bands at 2955 cm−1 and 3012 cm−1. Furthermore, quantitative information was obtained by calculating the average intensities of 25 randomly selected pixels. The final lipid content was paralleled four times for each group of samples.
Spotlight 400 ftir spectrometer
Manufactured by PerkinElmer
Sourced in United States
The Spotlight 400 FTIR spectrometer is a high-performance Fourier Transform Infrared (FTIR) spectroscopy system designed for a range of analytical applications. It provides precise infrared spectral data collection and analysis capabilities.
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5 protocols using spotlight 400 ftir spectrometer
1
Quantifying Lipid Composition in Hippocampus
2
FTIR Spectral Analysis of Samples
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FTIR spectra were analyzed in transmission mode by using Spotlight 400 FTIR spectrometer (PerkinElmer, USA) in the range of 400–4000 cm−1 at a resolution of 4 cm−1.
3
FTIR Characterization of Trpzip Hydrogels
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A Perkin Elmer Spotlight 400 FTIR spectrometer fitted with a diamond crystal attenuated total reflectance (ATR) accessory was used to acquire FTIR spectra. Trpzip hydrogels (3% w/v) were mounted between the diamond crystal and force lever and all spectra were scanned 20 times over the range of 2000–1000 cm−1.
4
Synthesis and Characterization of Metal-Organic Frameworks
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MIL-100 (Cr), MIL-100
(Fe), MIL-101 (Cr), and aluminum fumarate (AlFu) MOFs were synthesized
according to previous literature.30 (link)−33 (link) The details of the synthesis
are shown in theSupporting Information (SI) .
Powder X-ray diffraction patterns of the synthesized MOFs
were obtained by a PANalytical X’Pert Pro pw3040/60 diffractometer
with Cu Kα radiation operating at 45 kV and 40 mA. Brunauer–Emmett–Teller
(BET) surface area of the samples was collected on a Micromeritics
Tristar II at 77 K, and all samples were pretreated at 200 C for 15
h before measurement. Fourier transform infrared spectra (FTIR) of
powder samples was directly measured by a PerkinElmer Spotlight 400
FT-IR spectrometer with a range of 650–2500 cm–1. The morphology and particle size of the samples were determined
by scanning electron microscopy (SEM, JEOL, JSM-IT100). X-ray photoelectron
spectra (XPS) was collected using a ThermoFisher Al K-alpha apparatus
and scans were performed by a 400 μm spot size with an energy
step size of 0.2 eV. The thermal stability of four MOFs was investigated
by thermogravimetric analysis (TGA) using a Mettler-Toledo/STDA 851e
apparatus with a heating rate of 5 C/min.
(Fe), MIL-101 (Cr), and aluminum fumarate (AlFu) MOFs were synthesized
according to previous literature.30 (link)−33 (link) The details of the synthesis
are shown in the
Powder X-ray diffraction patterns of the synthesized MOFs
were obtained by a PANalytical X’Pert Pro pw3040/60 diffractometer
with Cu Kα radiation operating at 45 kV and 40 mA. Brunauer–Emmett–Teller
(BET) surface area of the samples was collected on a Micromeritics
Tristar II at 77 K, and all samples were pretreated at 200 C for 15
h before measurement. Fourier transform infrared spectra (FTIR) of
powder samples was directly measured by a PerkinElmer Spotlight 400
FT-IR spectrometer with a range of 650–2500 cm–1. The morphology and particle size of the samples were determined
by scanning electron microscopy (SEM, JEOL, JSM-IT100). X-ray photoelectron
spectra (XPS) was collected using a ThermoFisher Al K-alpha apparatus
and scans were performed by a 400 μm spot size with an energy
step size of 0.2 eV. The thermal stability of four MOFs was investigated
by thermogravimetric analysis (TGA) using a Mettler-Toledo/STDA 851e
apparatus with a heating rate of 5 C/min.
5
FTIR Analysis of Swelled Cellulose
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The functional groups’ change of swelled cellulose was analyzed by FTIR. The spectra were acquired on a Perkin Elmer Spotlight 400 FTIR spectrometer (Perkin Elmer, Waltham, MA, USA) working in a transmittance mode. Before scanning, the sample pellets were prepared by mixing with spectroscopic grade KBr in a ratio of 1:100 (w/w) and then pressed under vacuum. FTIR spectra were recorded at 4 cm−1 resolution and 64 scans per sample in the range of 4000–500 cm−1.
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