Lgr5 egfp ires creert2 mice

Male C57BL/6J mice, aged 6–8 weeks old, were purchased from Shanghai Sippe‐Bk Lab Animal Co., Ltd. (Shanghai, China). Lgr5‐EGFP‐IRES‐creERT2 mice, aged 6–8 weeks old, were purchased from Cyagen (Jiangsu, China). All mice were housed under controlled conditions of 20°C–22°C and relative humidity of 50%, a fixed 12 h light/dark cycle. Experimental protocols involving animals were approved by the Laboratory Animal Center of the Naval Medical University in conformance with the National Institute of Health Guide for the Care and Use of Laboratory Animals. Mice were assigned to three groups at random (1) Mice were administered by intragastric with NS (200 μL/mice) for 1 week. (2) Mice were administered by intragastric with NS (200 μL/mice) for 1 week before IR. (3) Mice were administered by intragastric with APS (900 mg/Kg, dissolved in NS) for 1 week before IR. At the end of the experiment, animals were injected intraperitoneally with pentobarbital sodium (1% v/v) for anaesthesia.

Lgr5‐eGFP‐IRES‐creERT2 mice (Cyagen Biosciences Inc.) and C57BL/6 WT mice (Beijing Vital River Laboratory Animal Technology Co., Ltd.) were used for the experiments. All the animal experiments in this study were approved by the Ethics Committee of the Health Science Center of Xi'an Jiaotong University. Mice were housed in plastic cages and were maintained on a 12‐h light–dark cycle at room temperature (22–26°C) with sterile pellet food and water ad libitum.