Il 1β ab254360

Manufactured by Abcam

Sourced in United States

IL-1β (ab254360) is a recombinant protein that corresponds to the human Interleukin-1 beta (IL-1β) protein. IL-1β is a pro-inflammatory cytokine involved in the regulation of immune and inflammatory responses.

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Lab products found in correlation

Gapdh 2118, by Cell Signaling Technology (1 mentions) Phosphatidylethanolamine assay kit, by Abcam (1 mentions) Masson s trichrome staining kit, by Leagene (1 mentions) Anti il18 a1115, by ABclonal (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions)

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IL-1β (390 citations) Ab254360 (48 citations)

3 protocols using il 1β ab254360

Comprehensive Antibody Purchasing Guide

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The antibodies for anti-NLRP3 (#15,101), ASC (#67,824), LC3-II (#4108), flotillin-1 (#3253), HSP70 (#4872), Cathepsin B (#31,718), p-p65 (#3033), p65 (#8242) and GAPDH (#2118) antibodies were purchased from Cell Signaling (Beverly, MA, USA); anti-caspase-1/p20 (22,915-1-AP) antibodies were purchased from Proteintech (Rosemont, IL 60018, USA); anti-LAMP-1 (NB120-19294), CD63 (NB100-77913) antibodies were purchased from Novus (Centennial CO 80112, USA); anti-TSG-101 (ab125011), p62 (ab109012), TNF-α (ab183218) and IL-1β (ab254360) antibodies were purchased from Abcam Inc. (Cambridge, MA, USA). anti-COX IV (tcna10591) was purchased from Taiclone Biotech Corp. (Taipei, Taiwan). The CD68 antibody (GTX41864) was purchased from GeneTex (Hsinchu City, Taiwan). The anti-iNOS (A0312) and anti-ARG1 (A4923) were purchased from ABclonal technology (Wobure. MA, USA). The HRP-conjugated anti-mouse (AB_10015289) and anti-rabbit (AB_2313567) secondary antibodies were purchased from Jackson ImmunoResearch Lab (West Grove, PA, USA).

Wang B.J., Chen Y.Y., Chang H.H., Chen R.J., Wang Y.J, & Lee Y.H. (2024). Zinc oxide nanoparticles exacerbate skin epithelial cell damage by upregulating pro-inflammatory cytokines and exosome secretion in M1 macrophages following UVB irradiation-induced skin injury. Particle and Fibre Toxicology, 21, 9.

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Oxidative Stress and Inflammation Assays

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Nitrotyrosine ELISA kit (ab113848) and Phosphatidylethanolamine Assay Kit (ab241005) was purchased from Abcam (Cambridge, MA). L-α-phosphatidylethanolamine (PE) (L130310) was purchased from Aladdin (Shanghai, China). Mito-SOX (M36008), BODIPY probe (D3835) and WGA (W11261) were purchased from Thermo Fisher Scientific (Waltham, USA). GSH and GSSG Assay Kit (S0053), Lipid Peroxidation MDA Assay Kit (S0131M), Hematoxylin and Eosin Staining Kit (C0105M) were purchased from Beyotime (Shanghai, China). Masson’s trichrome staining kit (DC0032) was purchased from Leagene (Beijing, China). NE ELISA kit (E-EL-0047c) was purchased from Elabscience (Wuhan, China). SC-siRNA (sc-37007); PLIN2-siRNA (sc-270227) were purchased from Santa Cruz Biotechnology (California, USA). Antibodies: PLIN2 (ab108323), iNOS (ab178945), Iba-1 (ab153696), Iba-1 (ab283342), PGP 9.5 (ab72911), TNF-α (ab205587), PCNA (ab29), 3-Nitrotyrosine (ab61392) and IL-1β (ab254360) were from Abcam (Cambridge, MA). c-fos (#2250, CST) was purchased from Cell Signaling Technology (Beverly, MA). 4-Hydroxynonenal (MA5-27570) was purchased from Thermo Fisher Scientific (Waltham, USA).

Zhang S., Hu L., Han C., Huang R., Ooi K., Qian X., Ren X., Chu D., Zhang H., Du D, & Xia C. (2021). PLIN2 Mediates Neuroinflammation and Oxidative/Nitrosative Stress via Downregulating Phosphatidylethanolamine in the Rostral Ventrolateral Medulla of Stressed Hypertensive Rats. Journal of Inflammation Research, 14, 6331-6348.

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Complement Activation Pathway Profiling

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Bovine serum albumin (BSA) and Rabbit anti-BSA IgG were purchased from sigma-Aldrich Chemical Co (StLouis, MO, USA). Anti-C3a(JF10-30) and anti-C3aR (NBP2-1569) were purchased from Novus Biologicals (Littleton, CO, United States). Anti-C5a (ab194637), anti-C5aR (ab59390) and IL-1β (ab254360) were obtained from Abcam (Cambridge, MA, United States). Anti-p-JAK2 (3771s), anti-JAK2 (3230s), anti-p-STAT3 (9145s), anti-STAT3 (12640s), anti-SOCS3 (9021s), anti-p-IKKα/β (2694s), anti- IKKα/β (8943s), anti-p- NF-κB p65 (3033s), anti- NF-κB p65 (8242s) were obtained from Cell Signaling Technologies (Beverly, MA, USA). Anti-IL-18 (A1115) was purchased from ABclonal Technology (Wuhan, China). The complement C5a(HY-P7695) and STAT3 activator Colivelin TFA(CAF, HY-P1061A) were obtained from MCE (Shanghai, China).

Li C., Li Y., Zhang H., Zhuo Y., Zhang L., Yang L., Gao Q., Tu Z., Shao R., Wang Y., Zhang J., Cui L, & Zhang S. (2022). Xuanfei Baidu Decoction suppresses complement overactivation and ameliorates IgG immune complex-induced acute lung injury by inhibiting JAK2/STAT3/SOCS3 and NF-κB signaling pathway. Phytomedicine, 109, 154551.

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