Cell rnaprotect

Manufactured by Qiagen

Cell RNAProtect is a reagent designed to stabilize and protect cellular RNA. It is used to collect and store cell samples for RNA analysis, preserving the RNA integrity prior to extraction and downstream processing.

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Lab products found in correlation

Facsaria fusion, by BD (2 mentions)

Spelling variants of this product

RNAprotect (311 citations) RNAprotect Cell Reagent (194 citations) RNAprotect Cell Mini Kit (2 citations)

2 protocols using cell rnaprotect

Isolation and Characterization of T Cell Subsets

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The remaining PBMC from each sample were used to sort for naïve and memory CD4⁺ and CD8⁺ T cells using fluorescent activated cell sorting. Cells were labelled by surface staining using T cell markers including CD3, CD4 and CD8 and memory markers CD27, CD45RA and CCR7 (Table S2 and Fig. S3). Naïve cells were denoted as those co-expressing CCR7, CD45RA and CD27 while the other remaining cells were regarded as memory cells. BD FACSAria Fusion (BD) was used for 4-way sorting of CD4 and CD8 naïve and memory T cells from each sample and collected directly into FCS. Sorted cells were centrifuged, resuspended in cell RNAProtect (Qiagen) and stored at −40 ^oC until processing for TCR sequencing.

Dzanibe S., Wilk A.J., Canny S., Ranganath T., Alinde B., Rubelt F., Huang H., Davis M.M., Holmes S.P., Jaspan H.B., Blish C.A, & Gray C.M. (2024). Premature skewing of T cell receptor clonality and delayed memory expansion in HIV-exposed infants. Nature Communications, 15, 4080.

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Fluorescence-Activated Cell Sorting for Naïve and Memory T Cells

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The remaining PBMC from each sample were used to sort for naïve and memory CD4⁺ and CD8⁺ T cells using fluorescent activated cell sorting. Cells were labelled by surface staining using T cell markers including CD3, CD4 and CD8 and memory markers CD27, CD45RA and CCR7 (Table S2 & Figure S3). Naïve cells were denoted as those co-expressing CCR7, CD45RA and CD27 while the other remaining cells were regarded as memory cells. A BD FACSAria Fusion (BD) was used for 4-way sorting of CD4 and CD8 naïve and memory T cells from each sample and collected directly into FCS. Sorted cells were centrifuged, resuspended in cell RNAProtect (Qiagen) and stored at −40°C until processing for TCR sequencing.

Dzanibe S., Wilk A.J., Canny S., Ranganath T., Alinde B., Rubelt F., Huang H., Davis M.M., Holmes S., Jaspan H.B., Blish C.A, & Gray C.M. (2023). Disrupted memory T cell expansion in HIV-exposed uninfected infants is preceded by premature skewing of T cell receptor clonality. bioRxiv.

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