Gaskets

Manufactured by Grace Bio-Labs

Gaskets are flexible sealing elements used to create a tight seal between two surfaces in various types of equipment and machinery. They are designed to prevent the leakage of fluids, gases, or other substances between joined components. Gaskets are typically made from a variety of materials, such as rubber, metal, or synthetic compounds, and are available in different shapes and sizes to accommodate different applications.

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Lab products found in correlation

Bovine serum albumin (bsa), by Merck Group (3 mentions) Thyroglobulin, by Merck Group (3 mentions) Acquiremp, by Refeyn (3 mentions) Discovermp, by Refeyn (3 mentions) Beta amylase, by Merck Group (2 mentions) Onemp, by Refeyn (1 mentions) Onemp instrument, by Refeyn (1 mentions) Twomp, by Refeyn (1 mentions)

Spelling variants of this product

Silicone gaskets (12 citations) CultureWell gasket (11 citations) CultureWell Reusable Gasket (9 citations) Silicon gaskets (5 citations) CoverWell perfusion chamber gaskets (3 citations) CultureWell Reusable Gaskets (CW-50R-1.0, (3 citations) Hybriwell gasket (3 citations) Silicone gasket well sheet (2 citations) CW-50R-1.0-CultureWell Gasket (2 citations)

4 protocols using gaskets

Mass Photometry Characterization of Nanobodies

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Select nanobodies were binned using mass photometry (MP). Experiments were performed on a Refeyn OneMP instrument (Refeyn Ltd). The instrument was calibrated with a mix of BSA (Sigma-Aldrich), thyroglobulin (Sigma-Aldrich) and beta-amylase (Sigma-Aldrich). Coverslips (Thorlabs) and gaskets (Grace Bio-Labs) were prepared by washing with 100% IPA followed by ddH₂O, repeated 3 times, followed by drying with HEPA filtered air. 12 μl of buffer was added to each well to focus the instrument after which 8 μl of protein solution was added and pipetted up and down to briefly mix after which movies/frame acquisition was promptly started. The final concentration in each experiment of recombinant Spike S1 monomer (Sinobiological) and each nanobody was 30 nM and between 25–40 nM respectively. Movies were acquired for 60 s (6000 frames) using AcquireMP (version 2.3.0; Refeyn Ltd) using standard settings. All movies were processed, analyzed, and masses estimated by fitting a Gaussian distribution to the data using DiscoverMP (version 2.3.0; Refeyn Ltd).

Mast F.D., Fridy P.C., Ketaren N.E., Wang J., Jacobs E.Y., Olivier J.P., Sanyal T., Molloy K.R., Schmidt F., Rutkowska M., Weisblum Y., Rich L.M., Vanderwall E.R., Dambrauskas N., Vigdorovich V., Keegan S., Jiler J.B., Stein M.E., Olinares P.D., Hatziioannou T., Sather D.N., Debley J.S., Fenyö D., Sali A., Bieniasz P.D., Aitchison J.D., Chait B.T, & Rout M.P. (2021). Nanobody Repertoires for Exposing Vulnerabilities of SARS-CoV-2. bioRxiv.

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Nanobody Mass Profiling by MP

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Select nanobodies were binned using MP. Experiments were performed on a Refeyn OneMP instrument (Refeyn Ltd). The instrument was calibrated with a mix of BSA (Sigma-Aldrich), thyroglobulin (Sigma-Aldrich), and beta-amylase (Sigma-Aldrich). Coverslips (Thorlabs) and gaskets (Grace Bio-Labs) were prepared by washing with 100% IPA followed by _ddH₂O, repeated three times, followed by drying with HEPA filtered air. 12 μl of buffer was added to each well to focus the instrument after which 8 μl of protein solution was added and pipetted up and down to briefly mix after which movies/frame acquisition was promptly started. The final concentration in each experiment of recombinant spike S1 monomer (Sino Biological) and each nanobody was 30 nM and between 25 and 40 nM, respectively. Movies were acquired for 60 s (6000 frames) using AcquireMP (version 2.3.0; Refeyn Ltd) using standard settings. All movies were processed, analyzed, and masses estimated by fitting a Gaussian distribution to the data using DiscoverMP (version 2.3.0; Refeyn Ltd).

Mast F.D., Fridy P.C., Ketaren N.E., Wang J., Jacobs E.Y., Olivier J.P., Sanyal T., Molloy K.R., Schmidt F., Rutkowska M., Weisblum Y., Rich L.M., Vanderwall E.R., Dambrauskas N., Vigdorovich V., Keegan S., Jiler J.B., Stein M.E., Olinares P.D., Herlands L., Hatziioannou T., Sather D.N., Debley J.S., Fenyö D., Sali A., Bieniasz P.D., Aitchison J.D., Chait B.T, & Rout M.P. (2021). Highly synergistic combinations of nanobodies that target SARS-CoV-2 and are resistant to escape. eLife, 10, e73027.

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Mass Photometry Analysis of Protein Oligomers

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Mass photometry data was acquired on a Refeyn One^MP instrument (Refeyn Ltd) as described previously with some modification⁷³. Briefly, the microscopic coverslips (Thorlabs) and gaskets (Grace bio-Labs) were cleaned with 100% IPA, followed by three times washing in ddH₂O and dried with HEPA-filtered air. For calibration, measurement of a mixture of known protein oligomer solutions of BSA, thyroglobulin, and beta-amylase (Sigma) were done with a final concentration of 40, 5, and 10 nM, respectively. For focusing the instrument, 12 µl of 2x PBS was added to each well followed by the addition of 8 µl TbRPA protein (final concentration = 20 nM). Movies were acquired for 60 s using Refeyn Acquire^MP (v. 2022 R1; Refeyn Ltd) using standard settings by the manufacturer. All movies were processed, analyzed, and mass was estimated by fitting a Gaussian distribution to the data using Refeyn Discover^MP (v. 2022 R1; Refeyn Ltd).

Mukherjee A., Hossain Z., Erben E., Ma S., Choi J.Y, & Kim H.S. (2023). Identification of a small-molecule inhibitor that selectively blocks DNA-binding by Trypanosoma brucei replication protein A1. Nature Communications, 14, 4390.

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Mass Photometry Analysis of CREB Constructs

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Mass photometry experiments were performed on a Refeyn TwoMP (Refeyn Ltd) calibrated with a mix of BSA (Sigma-Aldrich) and thyroglobulin (Sigma-Aldrich). Coverslips (Willco-Wells) and gaskets (Grace Biolabs) were prepared by washing with ddH₂O followed by isopropanol and again with ddH₂O, repeated three times, then dried. 20 μL of buffer was added to each well to focus the instrument, then 15 μL of buffer were removed and replaced with 15 μL of sample and mixed by pipette for 3 seconds before frame acquisition. Frames were acquired over 120 s using AcquireMP (version 2022 R1, Refeyn Ltd) using standard settings. Data were processed and analyzed by fitting a Gaussian distribution to the data using DiscoverMP (version 2022 R1, Refeyn Ltd).
We compared mass photometry measurements for each CREB construct after incubating the sample for 20, 30, and 45 minutes to assure that incubation times were long enough to reach equilibrium. All samples used to fit the dissociation constants were incubated for 1h before the measurement.

Li R.K., Ciblak M.A., Nordoff N., Pasarell L., Warnock D.W, & McGinnis M.R. (2000). In Vitro Activities of Voriconazole, Itraconazole, and Amphotericin B against Blastomyces dermatitidis, Coccidioides immitis, and Histoplasma capsulatum. Antimicrobial Agents and Chemotherapy, 44(6), 1734-1736.

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