Anti mouse foxp3 antibody

Manufactured by Abcam

The Anti-mouse FOXP3 antibody is a laboratory reagent used for the detection and quantification of the FOXP3 protein in mouse samples. FOXP3 is a transcription factor that is essential for the development and function of regulatory T cells. This antibody can be used in various immunological techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and localization of FOXP3 in mouse models.

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Lab products found in correlation

Leica dm5500b microscope, by Leica Microsystems (1 mentions) Neutral buffered formalin, by Thermo Fisher Scientific (1 mentions) Anti cd4 antibody, by Abcam (1 mentions)

2 protocols using anti mouse foxp3 antibody

Immunohistochemical Analysis of hAAT Expression

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Immunohistochemical staining to detect hAAT within myofibers as well as H&E staining was performed after tissues were fixed in 10% neutral buffered formalin for 24 h at room temperature (Fisher Scientific) and embedded in paraffin by University of Massachusetts Chan Medical School Morphology Core (Worcester, MA). Anti-AAT antibody (Fitzgerald, Acton, MA), anti-CD4 antibody (Abcam), and anti-mouse FOXP3 antibody (Abcam) were used as primary antibody. Images were acquired on a Leica DM5500 B microscope (Leica Microsystems, Wetzlar, Germany).

Arjomandnejad M., Sylvia K., Blackwood M., Nixon T., Tang Q., Muhuri M., Gruntman A.M., Gao G., Flotte T.R, & Keeler A.M. (2021). Modulating immune responses to AAV by expanded polyclonal T-regs and capsid specific chimeric antigen receptor T-regulatory cells. Molecular Therapy. Methods & Clinical Development, 23, 490-506.

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Quantifying FoxP3+ Cells in Jejunum

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Jejunal tissue was fixed in 10% formalin and processed into paraffin blocks. Jejunal tissue expression of FoxP3 was identified by immunohistochemistry (IHC) staining using anti-mouse FoxP3 antibody (Abcam, Cambridge, Mass). Quantification of stained FoxP3-positive cells per square millimeter of lamina propria was performed with an Olympus microscope linked to the National Institutes of Health Image Analysis Program (NIH, Bethesda, MD).

Wang M., Yang I.V., Davidson E.J., Joetham A., Takeda K., O’Connor B.P, & Gelfand E.W. (2017). Forkhead Box Protein 3 (FoxP3) Demethylation is Associated with Tolerance Induction in Peanut-Induced Intestinal Allergy. The Journal of allergy and clinical immunology, 141(2), 659-670.e2.

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