MCF-7 cells, derived from female mammary gland adenocarcinoma tissue, were purchased from the ATCC (HTB-22) and grown in DMEM (high glucose) supplemented with 10% Fetal Bovine Serum (FBS) and 1% penicillin-streptomycin. Cells were maintained under standard growth conditions: 37°C and 5% CO2 in a humidified atmosphere. Heat shock was at 43°C for the indicated times. Acidosis was at pH 6.0 in 1% O2 (hypoxia). Hypoxia was induced by incubating cells in an H35 HypoOxystation at 37°C in a 1% O2, 5% CO2 and N2-balanced environment. Sodium arsenite (125 mM), H2O2 (300 nM) and thapsigargin (300 nM), were used for the indicated times. 1.4nm Nanogold® negatively charged nanoparticles (Nanoprobes, #2023) or neutral nanoparticles (Nanoprobes, #2010) were directly added to the medium at 300 μM.
Nanogold negatively charged nanoparticles
Manufactured by Nanoprobes
Nanogold® negatively charged nanoparticles are spherical gold nanoparticles with a negative surface charge. They are synthesized using a proprietary process. The core function of these nanoparticles is to serve as a versatile tool for various applications, including electron microscopy, flow cytometry, and bioassays.
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2 protocols using nanogold negatively charged nanoparticles
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MCF-7 Cell Stress Responses
2
MCF-7 Cell Stress Responses
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MCF-7 cells, derived from female mammary gland adenocarcinoma tissue, were purchased from the ATCC (HTB-22) and grown in DMEM (high glucose) supplemented with 10% Fetal Bovine Serum (FBS) and 1% penicillin-streptomycin. Cells were maintained under standard growth conditions: 37°C and 5% CO2 in a humidified atmosphere. Heat shock was at 43°C for the indicated times. Acidosis was at pH 6.0 in 1% O2 (hypoxia). Hypoxia was induced by incubating cells in an H35 HypoOxystation at 37°C in a 1% O2, 5% CO2 and N2-balanced environment. Sodium arsenite (125 mM), H2O2 (300 nM) and thapsigargin (300 nM), were used for the indicated times. 1.4nm Nanogold® negatively charged nanoparticles (Nanoprobes, #2023) or neutral nanoparticles (Nanoprobes, #2010) were directly added to the medium at 300 μM.
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